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Blood, Vol. 93 No. 1 (January 1), 1999:
pp. 394-398
Peripheral Blood CD14+ Cells From Healthy Subjects
Carry a Circular Conformation of Latent Cytomegalovirus
Genome
Cynthia A. Bolovan-Fritts,
Edward S. Mocarski, and
Jean A. Wiedeman
From the Department of Pediatrics, Section of Infectious Diseases,
University of California Davis; and the Department of Microbiology and
Immunology, Stanford University School of Medicine, Stanford, CA.
The majority of the human population harbors latent cytomegalovirus.
Although CD14+ peripheral blood mononuclear cells have
been implicated as sites of latency, the conformation of the latent
viral genome in these cells is unknown. In this study, the conformation
of viral genomic DNA was assessed in CD14+ cells from
healthy virus seropositive carriers using an electrophoretic separation
on native agarose gels in combination with polymerase chain reaction
detection. Here we show that the viral genome migrates as a circular
plasmid with a mobility equivalent to a circular 230-kb Shigella
flexneri megaplasmid marker. Neither linear nor complex or
integrated forms of the viral genome were detected. This report
provides further evidence that the CD14+ cell population
is an important site of viral latency in the naturally infected human
host. Detection of the viral genome as a circular plasmid during
latency suggests that this virus maintains its genome in a manner
analogous to other herpesviruses where latent viral genome conformation
has been studied.
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