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Blood, Vol. 93 No. 10 (May 15), 1999:
pp. 3338-3346
Characterization of Mast Cell-Committed Progenitors Present in
Human Umbilical Cord Blood
Duraisamy Kempuraj,
Hirohisa Saito,
Azusa Kaneko,
Kazumi Fukagawa,
Masaharu Nakayama,
Hano Toru,
Morimitsu Tomikawa,
Hiroshi Tachimoto,
Motohiro Ebisawa,
Akira Akasawa,
Toko Miyagi,
Hiromitsu Kimura,
Toshiharu Nakajima,
Kohichiro Tsuji, and
Tatsutoshi Nakahata
From the Department of Allergy and the Department of Experimental
Surgery, National Children's Medical Research Center, Tokyo, Japan;
the Department of Clinical Oncology, The Institute of Medical Science,
University of Tokyo, Tokyo, Japan; and the Department of Bioregulatory
Function, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Human mast cells are derived from CD34+ hematopoietic
cells present in cord blood, bone marrow, and peripheral blood.
However, little is known about the properties of the
CD34+ cells. We demonstrated here that mast cell
progenitors that have distinct phenotypes from other hematopoietic cell
types are present in cord blood by culturing single, sorted
CD34+ cells in 96-well plates or unsorted cells in
methylcellulose. The CD34+ mast cell-committed
progenitors often expressed CD38 and often lacked HLA-DR, whereas
CD34+ erythroid progenitors often expressed both CD38 and
HLA-DR and CD34+ granulocyte-macrophage progenitors often
had CD33 and sometimes expressed CD38. We then cultured single cord
blood-derived CD34+CD38+ cells under
conditions optimal for mast cells and three types of myeloid cells, ie,
basophils, eosinophils, and macrophages. Of 1,200 CD34+CD38+ cells, we were able to detect 13 pure mast cell colonies and 52 pure colonies consisting of either one
of these three myeloid cell types. We found 17 colonies consisting of
two of the three myeloid cell types, whereas only one colony consisted
of mast cells and another cell type. These results indicate that human mast cells develop from progenitors that have unique phenotypes and
that committed mast cell progenitors develop from multipotent hematopoietic cells through a pathway distinct from myeloid lineages including basophils, which have many similarities to mast cells.
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