|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 10 (May 15), 1999:
pp. 3391-3400
Characterization of HOX Gene Expression During Myelopoiesis: Role of
HOX A5 in Lineage Commitment and Maturation
John F. Fuller,
Jeanne McAdara,
Yifah Yaron,
Mark Sakaguchi,
John
K. Fraser, and
Judith C. Gasson
From the Department of Biological Chemistry, UCLA School of Medicine,
the Division of Hematology-Oncology, Department of Medicine, the
Jonsson Comprehensive Cancer Center, and the Molecular Biology
Institute, UCLA, Los Angeles, CA.
During the process of normal hematopoiesis, proliferation is tightly
linked to maturation. The molecular mechanisms that lead to production
of mature effector cells with a variety of phenotypes and functions
from a single multipotent progenitor are only beginning to be
elucidated. It is important to determine how these maturation events
are regulated at the molecular level, because this will provide
significant insights into the process of normal hematopoiesis as well
as leukemogenesis. Transcription factors containing the highly
conserved homeobox motif show considerable promise as potential regulators of hematopoietic maturation events. In this study, we
focused on identification and characterization of homeobox genes of the
HOX family that are important in regulating normal human myeloid
differentiation induced by the hematopoietic growth factor,
granulocyte-macrophage colony-stimulating factor (GM-CSF). We have
identified three homeobox genes, HOX A5, HOX B6, and HOX B7, which are
expressed during early myelopoiesis. Treating bone marrow cells with
antisense oligodeoxynucleotides to HOX A5 resulted in inhibition of
granulocytic/monocytic hematopoiesis and increased the generation of
erythroid progenitors. Also, overexpression of HOX A5 inhibited
erythroid differentiation of the K562 cell line. Based on these
observations, we propose that HOX A5 functions as an important
regulator of hematopoietic lineage determination and maturation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. Strathdee, T. L. Holyoake, A. Sim, A. Parker, D. G. Oscier, J. V. Melo, S. Meyer, T. Eden, A. M. Dickinson, J. C. Mountford, et al.
Inactivation of HOXA Genes by Hypermethylation in Myeloid and Lymphoid Malignancy is Frequent and Associated with Poor Prognosis
Clin. Cancer Res.,
September 1, 2007;
13(17):
5048 - 5055.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Strathdee, A. Sim, R. Soutar, T. L. Holyoake, and R. Brown
HOXA5 is targeted by cell-type-specific CpG island methylation in normal cells and during the development of acute myeloid leukaemia
Carcinogenesis,
February 1, 2007;
28(2):
299 - 309.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Y. Chung, G. Morrone, J. J. Schuringa, M. Plasilova, J.-H. Shieh, Y. Zhang, P. Zhou, and M. A.S. Moore
Enforced Expression of NUP98-HOXA9 in Human CD34+ Cells Enhances Stem Cell Proliferation
Cancer Res.,
December 15, 2006;
66(24):
11781 - 11791.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Shivdasani
MicroRNAs: regulators of gene expression and cell differentiation
Blood,
December 1, 2006;
108(12):
3646 - 3653.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhai, H. Lin, R. Canete-Soler, and W. W. Schlaepfer
HoxB2 binds mutant SOD1 and is altered in transgenic model of ALS
Hum. Mol. Genet.,
September 15, 2005;
14(18):
2629 - 2640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Chen, E. Rubin, H. Zhang, S. Chung, C. C. Jie, E. Garrett, S. Biswal, and S. Sukumar
Identification of Transcriptional Targets of HOXA5
J. Biol. Chem.,
May 13, 2005;
280(19):
19373 - 19380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Fischbach, S. Rozenfeld, W. Shen, S. Fong, D. Chrobak, D. Ginzinger, S. C. Kogan, A. Radhakrishnan, M. M. Le Beau, C. Largman, et al.
HOXB6 overexpression in murine bone marrow immortalizes a myelomonocytic precursor in vitro and causes hematopoietic stem cell expansion and acute myeloid leukemia in vivo
Blood,
February 15, 2005;
105(4):
1456 - 1466.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Ghannam, A. Takeda, T. Camarata, M. A. Moore, A. Viale, and N. R. Yaseen
The Oncogene Nup98-HOXA9 Induces Gene Transcription in Myeloid Cells
J. Biol. Chem.,
January 9, 2004;
279(2):
866 - 875.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Thompson, M. F. Quinn, D. Grimwade, C. M. O'Neill, M. R. Ahmed, S. Grimes, M. F. McMullin, F. Cotter, and T. R. J. Lappin
Global down-regulation of HOX gene expression in PML-RARalpha + acute promyelocytic leukemia identified by small-array real-time PCR
Blood,
February 15, 2003;
101(4):
1558 - 1565.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. M. Owens and R. G. Hawley
HOX and Non-HOX Homeobox Genes in Leukemic Hematopoiesis
Stem Cells,
September 1, 2002;
20(5):
364 - 379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Taghon, F. Stolz, M. De Smedt, M. Cnockaert, B. Verhasselt, J. Plum, and G. Leclercq
HOX-A10 regulates hematopoietic lineage commitment: evidence for a monocyte-specific transcription factor
Blood,
February 15, 2002;
99(4):
1197 - 1204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yaron, J. K. McAdara, M. Lynch, E. Hughes, and J. C. Gasson
Identification of Novel Functional Regions Important for the Activity of HOXB7 in Mammalian Cells
J. Immunol.,
April 15, 2001;
166(8):
5058 - 5067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Buske, M. Feuring-Buske, J. Antonchuk, P. Rosten, D. E. Hogge, C. J. Eaves, and R. K. Humphries
Overexpression of HOXA10 perturbs human lymphomyelopoiesis in vitro and in vivo
Blood,
April 15, 2001;
97(8):
2286 - 2292.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. A. Golpon, M. W. Geraci, M. D. Moore, H. L. Miller, G. J. Miller, R. M. Tuder, and N. F. Voelkel
HOX Genes in Human Lung : Altered Expression in Primary Pulmonary Hypertension and Emphysema
Am. J. Pathol.,
March 1, 2001;
158(3):
955 - 966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Lekstrom-Himes
The Role of C/EBP{{varepsilon}} in the Terminal Stages of Granulocyte Differentiation
Stem Cells,
February 1, 2001;
19(2):
125 - 133.
[Abstract]
[Full Text]
|
|
|
| |
