|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 11 (June 1), 1999:
pp. 3831-3838
Endothelial Cells Undergoing Apoptosis Become Proadhesive for
Nonactivated Platelets
Thomas Bombeli,
Barbara R. Schwartz, and
John M. Harlan
From the Division of Hematology, University of
Washington, Seattle, WA; and the Division of Hematology, the Department
of Medicine, University Hospital of Zurich, Zurich, Switzerland.
Under normal conditions, platelets do not adhere to endothelium.
However, when platelets or endothelial cells are stimulated by thrombin
or cytokines, respectively, platelets bind avidly to endothelium.
Because there is accumulating evidence that endothelial cells may
become apoptotic under certain proinflammatory or prothrombotic conditions, we investigated whether endothelial cells undergoing apoptosis may become proadhesive for nonactivated platelets. Human umbilical vein endothelial cells (HUVEC) were induced to undergo apoptosis by staurosporine, a nonspecific protein kinase inhibitor, or
by culture in suspension with serum-deprivation. After treatment of
HUVEC or platelets with different receptor antagonists, nonactivated, washed human platelets were allowed to adhere to HUVEC for 20 minutes.
To exclude matrix involvement, platelet binding was measured in
suspension by using flow cytometry. Independent of the method of
apoptosis induction, there was a marked increase in platelet binding to
apoptotic HUVEC. Although HUVEC exhibited maximal adhesiveness for
platelets after 2 to 4 hours, complete DNA fragmentation of HUVEC
occurred only several hours later. Adhesion assays after blockade of
different platelet receptors showed only involvement of
 1-integrins. Platelet binding to apoptotic HUVEC was
inhibited by more than 70% when platelets were treated with blocking
anti-1 antibodies. Treatment of apoptotic HUVEC with
blocking antibodies to different potential platelet receptors,
including known ligands for 1-integrins, did not affect
platelet binding. As assessed by determination of -thromboglobulin
and platelet factor 4 in the supernatants, platelets bound to apoptotic
HUVEC became slightly activated. However, significant expression of
platelet P-selectin (CD62P) was not found. These data provide further
evidence that endothelial cells undergoing apoptosis may contribute to
thrombotic events.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Mihov, N. Bogdanov, B. Grenacher, M. Gassmann, G. Zund, A. Bogdanova, and R. Tavakoli
Erythropoietin protects from reperfusion-induced myocardial injury by enhancing coronary endothelial nitric oxide production
Eur. J. Cardiothorac. Surg.,
May 1, 2009;
35(5):
839 - 846.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. C. Johnson, S. Corthals, C. Ramos, A. Hoering, K. Cocks, N. J. Dickens, J. Haessler, H. Goldschmidt, J. A. Child, S. E. Bell, et al.
Genetic associations with thalidomide mediated venous thrombotic events in myeloma identified using targeted genotyping
Blood,
December 15, 2008;
112(13):
4924 - 4934.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D J Hausenloy and D M Yellon
Targeting residual cardiovascular risk: raising high-density lipoprotein cholesterol levels
Postgrad. Med. J.,
November 1, 2008;
84(997):
590 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Le, R. Damico, M. Damarla, A. Boueiz, H. H. Pae, J. Skirball, E. Hasan, X. Peng, A. Chesley, M. T. Crow, et al.
Alveolar cell apoptosis is dependent on p38 MAP kinase-mediated activation of xanthine oxidoreductase in ventilator-induced lung injury
J Appl Physiol,
October 1, 2008;
105(4):
1282 - 1290.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D J Hausenloy and D M Yellon
Targeting residual cardiovascular risk: raising high-density lipoprotein cholesterol levels
Heart,
June 1, 2008;
94(6):
706 - 714.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Bakouboula, O. Morel, A. Faure, F. Zobairi, L. Jesel, A. Trinh, M. Zupan, M. Canuet, L. Grunebaum, A. Brunette, et al.
Procoagulant Membrane Microparticles Correlate with the Severity of Pulmonary Arterial Hypertension
Am. J. Respir. Crit. Care Med.,
March 1, 2008;
177(5):
536 - 543.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Liu, B. Schwartz, Y. Tsubota, E. Raines, H. Kiyokawa, K. Yonekawa, J. M. Harlan, and L. M. Schnapp
Cyclin-Dependent Kinase Inhibitors Block Leukocyte Adhesion and Migration
J. Immunol.,
February 1, 2008;
180(3):
1808 - 1817.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-Y. Chong and G. Y.H. Lip
Viewpoint: The prothrombotic state in heart failure: A maladaptive inflammatory response?
Eur J Heart Fail,
February 1, 2007;
9(2):
124 - 128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Mineo, H. Deguchi, J. H. Griffin, and P. W. Shaul
Endothelial and Antithrombotic Actions of HDL
Circ. Res.,
June 9, 2006;
98(11):
1352 - 1364.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Nicholls and S. L. Hazen
Myeloperoxidase and Cardiovascular Disease
Arterioscler Thromb Vasc Biol,
June 1, 2005;
25(6):
1102 - 1111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Durand, A. Scoazec, A. Lafont, J. Boddaert, A. Al Hajzen, F. Addad, M. Mirshahi, M. Desnos, A. Tedgui, and Z. Mallat
In Vivo Induction of Endothelial Apoptosis Leads to Vessel Thrombosis and Endothelial Denudation: A Clue to the Understanding of the Mechanisms of Thrombotic Plaque Erosion
Circulation,
June 1, 2004;
109(21):
2503 - 2506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kobayashi, N. Ouchi, S. Kihara, K. Walsh, M. Kumada, Y. Abe, T. Funahashi, and Y. Matsuzawa
Selective Suppression of Endothelial Cell Apoptosis by the High Molecular Weight Form of Adiponectin
Circ. Res.,
March 5, 2004;
94
(4):
e27 - e31.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gawaz
Role of platelets in coronary thrombosis and reperfusion of ischemic myocardium
Cardiovasc Res,
February 15, 2004;
61(3):
498 - 511.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.A. Stouffer and S.S. Smyth
Effects of Thrombin on Interactions Between {beta}3-Integrins and Extracellular Matrix in Platelets and Vascular Cells
Arterioscler Thromb Vasc Biol,
November 1, 2003;
23(11):
1971 - 1978.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kojima, H. Kanada, S. Shimizu, E. Kasama, K. Shibuya, H. Nakauchi, T. Nagasawa, and A. Shibuya
CD226 Mediates Platelet and Megakaryocytic Cell Adhesion to Vascular Endothelial Cells
J. Biol. Chem.,
September 19, 2003;
278(38):
36748 - 36753.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Lagadec, O. Dejoux, M. Ticchioni, F. Cottrez, M. Johansen, E. J. Brown, and A. Bernard
Involvement of a CD47-dependent pathway in platelet adhesion on inflamed vascular endothelium under flow
Blood,
June 15, 2003;
101(12):
4836 - 4843.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Y. Liu, A. K. Death, and D. J. Handelsman
Androgens and Cardiovascular Disease
Endocr. Rev.,
June 1, 2003;
24(3):
313 - 340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. C. Aird
The role of the endothelium in severe sepsis and multiple organ dysfunction syndrome
Blood,
May 15, 2003;
101(10):
3765 - 3777.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Wernig, M. Mayr, and Q. Xu
Mechanical Stretch-Induced Apoptosis in Smooth Muscle Cells Is Mediated by {beta}1-Integrin Signaling Pathways
Hypertension,
April 1, 2003;
41(4):
903 - 911.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Valgimigli, L. Agnoletti, S. Curello, L. Comini, G. Francolini, F. Mastrorilli, E. Merli, R. Pirani, G. Guardigli, P. G. Grigolato, et al.
Serum From Patients With Acute Coronary Syndromes Displays a Proapoptotic Effect on Human Endothelial Cells: A Possible Link to Pan-Coronary Syndromes
Circulation,
January 21, 2003;
107(2):
264 - 270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. E. Holzknecht, K. L. Kuypers, T. B. Plummer, J. Williams, M. Bustos, G. J. Gores, G. J. Brunn, and J. L. Platt
Apoptosis and Cellular Activation in the Pathogenesis of Acute Vascular Rejection
Circ. Res.,
December 13, 2002;
91(12):
1135 - 1141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S Ahmed, R K Siddiqui, A K Siddiqui, S A Zaidi, and J Cervia
HIV associated thrombotic microangiopathy
Postgrad. Med. J.,
September 1, 2002;
78(923):
520 - 524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ling, A. Dai, M. R. I. Williams, K. Myles, R. J. Dilley, P. A. Komesaroff, and K. Sudhir
Testosterone (T) Enhances Apoptosis-Related Damage in Human Vascular Endothelial Cells
Endocrinology,
March 1, 2002;
143(3):
1119 - 1125.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Cailhier, L. Nolin, and M.-J. Hebert
Thrombotic microangiopathy following renal ischaemia and revascularization: apoptosis of endothelial cells in action
Nephrol. Dial. Transplant.,
August 1, 2001;
16(8):
1725 - 1726.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Mallat and A. Tedgui
Current Perspective on the Role of Apoptosis in Atherothrombotic Disease
Circ. Res.,
May 25, 2001;
88(10):
998 - 1003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Andre, C. V. Denis, J. Ware, S. Saffaripour, R. O. Hynes, Z. M. Ruggeri, and D. D. Wagner
Platelets adhere to and translocate on von Willebrand factor presented by endothelium in stimulated veins
Blood,
November 15, 2000;
96(10):
3322 - 3328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Aebert, S. Kirchner, A. Keyser, D. E. Birnbaum, E. Holler, R. Andreesen, and G. Eissner
Endothelial apoptosis is induced by serum of patients after cardiopulmonary bypass
Eur. J. Cardiothorac. Surg.,
November 1, 2000;
18(5):
589 - 593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Brouard, L. E. Otterbein, J. Anrather, E. Tobiasch, F. H. Bach, A. M.K. Choi, and M. P. Soares
Carbon Monoxide Generated by Heme Oxygenase 1 Suppresses Endothelial Cell Apoptosis
J. Exp. Med.,
October 2, 2000;
192(7):
1015 - 1026.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Tricot, Z. Mallat, C. Heymes, J. Belmin, G. Leseche, and A. Tedgui
Relation Between Endothelial Cell Apoptosis and Blood Flow Direction in Human Atherosclerotic Plaques
Circulation,
May 30, 2000;
101(21):
2450 - 2453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-M. Wu, C.-L. Huang, H.-J. Kung, and C.-Y. F. Huang
Proteolytic Activation of Etk/Bmx Tyrosine Kinase by Caspases
J. Biol. Chem.,
May 18, 2001;
276(21):
17672 - 17678.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
