|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 11 (June 1), 1999:
pp. 3847-3855
Collagen Mediates Changes in Intracellular Calcium in Primary Mouse
Megakaryocytes Through syk-Dependent and -Independent Pathways
Stephen J. Briddon,
Steven K. Melford,
Martin Turner,
Victor Tybulewicz, and
Steve P. Watson
From the Department of Pharmacology, University of Oxford, Oxford,
UK; and the National Institute for Medical Research, London, UK.
We have characterized changes in [Ca2+]i
in primary mouse megakaryocytes in response to fibrillar collagen and
in response to cross-linking of the collagen receptor, the integrin
 2 1. The response to collagen was markedly
different from that seen to a triple helical collagen-related peptide
(CRP), which signals via the tyrosine kinases p59fyn and
p72syk. This peptide binds to the collagen receptor
glycoprotein VI (GPVI), but not to the integrin
21. Collagen elicited a sustained increase in [Ca2+]i composed primarily of
influx of extracellular Ca2+ with some Ca2+
release from internal stores. In contrast to CRP, this response was
only partially (~30%) inhibited by the src-family kinase inhibitor PP1 (10 µmol/L) or by microinjection of the tandem SH2 domains of
p72syk. Collagen also caused an increase in
[Ca2+]i in megakaryocytes deficient in
either p59fyn or p72syk, although the response
was reduced by approximately 40% in both cases: Cross-linking of the
2 integrin increased [Ca2+]i
in these cells exclusively via Ca2+ influx. This response
was reduced by approximately 50% after PP1 pretreatment, but was
significantly increased in fyn-deficient megakaryocytes. Collagen
therefore increases [Ca2+]i in mouse
megakaryocytes via multiple receptors, including GPVI, which causes
Ca2+ mobilization, and 21,
which stimulates a substantial influx of extracellular
Ca2+.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Nishi, E. D. Werner, B.-C. Oh, J. D. Frantz, S. Dhe-Paganon, L. Hansen, J. Lee, and S. E. Shoelson
Kinase Activation through Dimerization by Human SH2-B
Mol. Cell. Biol.,
April 1, 2005;
25(7):
2607 - 2621.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Talmor-Cohen, R Tomashov-Matar, E Eliyahu, R Shapiro, and R Shalgi
Are Src family kinases involved in cell cycle resumption in rat eggs?
Reproduction,
April 1, 2004;
127(4):
455 - 463.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Martinez-Pinna, G. Tolhurst, I. S. Gurung, J. I. Vandenberg, and M. P. Mahaut-Smith
Sensitivity limits for voltage control of P2Y receptor-evoked Ca2+ mobilization in the rat megakaryocyte
J. Physiol.,
February 15, 2004;
555(1):
61 - 70.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Patil, D. K. Newman, and P. J. Newman
Platelet endothelial cell adhesion molecule-1 serves as an inhibitory receptor that modulates platelet responses to collagen
Blood,
March 15, 2001;
97(6):
1727 - 1732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-M. Pasquet, L. Quek, S. Pasquet, A. Poole, J. R. Matthews, C. Lowell, and S. P. Watson
Evidence of a Role for SHP-1 in Platelet Activation by the Collagen Receptor Glycoprotein VI
J. Biol. Chem.,
September 8, 2000;
275(37):
28526 - 28531.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-H. Lagrue-Lak-Hal, N. Debili, G. Kingbury, C. Lecut, J.-P. Le Couedic, J.-L. Villeval, M. Jandrot-Perrus, and W. Vainchenker
Expression and Function of the Collagen Receptor GPVI during Megakaryocyte Maturation
J. Biol. Chem.,
April 27, 2001;
276(18):
15316 - 15325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
