|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 12 (June 15), 1999:
pp. 4167-4178
Expression of p21Cip1/Waf1/Sdi1 and p27Kip1
Cyclin-Dependent Kinase Inhibitors During Human Hematopoiesis
Toshiyasu Taniguchi,
Hisako Endo,
Norio Chikatsu,
Kaoru Uchimaru,
Shigetaka Asano,
Toshiro Fujita,
Tatsutoshi Nakahata, and
Toru Motokura
From the Fourth Department of Internal Medicine, the Department of
Pathology, the Branch Hospital, School of Medicine, and the Departments
of Hematology/Oncology and Clinical Oncology, Institute of Medical
Science, University of Tokyo, Tokyo, Japan.
Expression of p21 and p27 cyclin-dependent kinase inhibitors is
associated with induced differentiation and cell-cycle arrest in some
hematopoietic cell lines. However, it is not clear how these inhibitors
are expressed during normal hematopoiesis. We examined various human
hematopoietic colonies derived from cord blood CD34+
cells, bone marrow, and peripheral blood cells using a quantitative reverse transcription-polymerase chain reaction assay, immunochemistry, and/or Western blot analysis. p21 mRNA was expressed increasingly over
time in all of the colonies examined (granulocytes, macrophages, megakaryocytes, and erythroblasts), whereas p27 mRNA levels remained low, except for erythroid bursts. Erythroid bursts expressed both p21
and p27 mRNAs with differentiation but expressed neither protein, whereas both proteins were expressed in megakaryocytes and peripheral blood monocytes. In bone marrow, p21 was immunostained almost exclusively in a subset of megakaryocytes and p27 protein was present
in megakaryocytes, plasma cells, and endothelial cells. In
megakaryocytes, reciprocal expression of p27 to Ki-67 was evident and
an inverse relationship between p21 and Ki-67 positivities was also
present, albeit less obvious. These observations suggest that a complex
lineage-specific regulation is involved in p21 and p27 expression and
that these inhibitors are involved in cell-cycle exit in megakaryocytes.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Ksiazek, J. Mikula-Pietrasik, S. Olijslagers, A. Jorres, T. von Zglinicki, and J. Witowski
Vulnerability to oxidative stress and different patterns of senescence in human peritoneal mesothelial cell strains
Am J Physiol Regulatory Integrative Comp Physiol,
February 1, 2009;
296(2):
R374 - R382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bedi, J. Du, A. K. Sharma, I. Gomes, and S. J. Ackerman
Human C/EBP-{epsilon} activator and repressor isoforms differentially reprogram myeloid lineage commitment and differentiation
Blood,
January 8, 2009;
113(2):
317 - 327.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Acosta, N. Ferrandiz, G. Bretones, V. Torrano, R. Blanco, C. Richard, B. O'Connell, J. Sedivy, M. D. Delgado, and J. Leon
Myc Inhibits p27-Induced Erythroid Differentiation of Leukemia Cells by Repressing Erythroid Master Genes without Reversing p27-Mediated Cell Cycle Arrest
Mol. Cell. Biol.,
December 15, 2008;
28(24):
7286 - 7295.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Banerjee, M. Sieburg, E. Samuelson, and G. Feuer
Human T-Cell Lymphotropic Virus Type 1 Infection of CD34+ Hematopoietic Progenitor Cells Induces Cell Cycle Arrest by Modulation of p21cip1/waf1 and Survivin
Stem Cells,
December 1, 2008;
26(12):
3047 - 3058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Gilles, R. Guieze, D. Bluteau, V. Cordette-Lagarde, C. Lacout, R. Favier, F. Larbret, N. Debili, W. Vainchenker, and H. Raslova
P19INK4D links endomitotic arrest and megakaryocyte maturation and is regulated by AML-1
Blood,
April 15, 2008;
111(8):
4081 - 4091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. De Franceschi, L. Ronzoni, M. D. Cappellini, F. Cimmino, A. Siciliano, S. L. Alper, V. Servedio, C. Pozzobon, and A. Iolascon
K-CL co-transport plays an important role in normal and {beta} thalassemic erythropoiesis
Haematologica,
October 1, 2007;
92(10):
1319 - 1326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Muntean, L. Pang, M. Poncz, S. F. Dowdy, G. A. Blobel, and J. D. Crispino
Cyclin D-Cdk4 is regulated by GATA-1 and required for megakaryocyte growth and polyploidization
Blood,
June 15, 2007;
109(12):
5199 - 5207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Pelayo, K. Miyazaki, J. Huang, K. P. Garrett, D. G. Osmond, and P. W. Kincade
Cell Cycle Quiescence of Early Lymphoid Progenitors in Adult Bone Marrow
Stem Cells,
December 1, 2006;
24(12):
2703 - 2713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Soeiro, A. Mohamedali, H. M. Romanska, N. C. Lea, E. S. Child, J. Glassford, S. J. Orr, C. Roberts, K. N. Naresh, E.-N. Lalani, et al.
p27Kip1 and p130 Cooperate To Regulate Hematopoietic Cell Proliferation In Vivo.
Mol. Cell. Biol.,
August 1, 2006;
26(16):
6170 - 6184.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ksiazek, K. Piwocka, A. Brzezinska, E. Sikora, M. Zabel, A. Breborowicz, A. Jorres, and J. Witowski
Early loss of proliferative potential of human peritoneal mesothelial cells in culture: the role of p16INK4a-mediated premature senescence
J Appl Physiol,
March 1, 2006;
100(3):
988 - 995.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tripp, P. Banerjee, M. Sieburg, V. Planelles, F. Li, and G. Feuer
Induction of Cell Cycle Arrest by Human T-Cell Lymphotropic Virus Type 1 Tax in Hematopoietic Progenitor (CD34+) Cells: Modulation of p21cip1/waf1 and p27kip1 Expression
J. Virol.,
November 15, 2005;
79(22):
14069 - 14078.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. W. Krauss, A. J. Lo, S. A. Short, M. J. Koury, N. Mohandas, and J. A. Chasis
Nuclear substructure reorganization during late-stage erythropoiesis is selective and does not involve caspase cleavage of major nuclear substructural proteins
Blood,
September 15, 2005;
106(6):
2200 - 2205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Munoz-Alonso, J. C. Acosta, C. Richard, M. D. Delgado, J. Sedivy, and J. Leon
p21Cip1 and p27Kip1 Induce Distinct Cell Cycle Effects and Differentiation Programs in Myeloid Leukemia Cells
J. Biol. Chem.,
May 6, 2005;
280(18):
18120 - 18129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Chinami, Y. Yano, X. Yang, S. Salahuddin, K. Moriyama, M. Shiroishi, H. Turner, T. Shirakawa, and C. N. Adra
Binding of HTm4 to Cyclin-dependent Kinase (Cdk)-associated Phosphatase (KAP){middle dot}Cdk2{middle dot}Cyclin A Complex Enhances the Phosphatase Activity of KAP, Dissociates Cyclin A, and Facilitates KAP Dephosphorylation of Cdk2
J. Biol. Chem.,
April 29, 2005;
280(17):
17235 - 17242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Maria-Engler, M. L. C Correa-Giannella, L. Labriola, K. Krogh, C. Colin, F. H. Lojudice, C. A. M. Aita, E. M. C. de Oliveira, T. C S. Correa, I. C. da Silva, et al.
Co-localization of nestin and insulin and expression of islet cell markers in long-term human pancreatic nestin-positive cell cultures
J. Endocrinol.,
December 1, 2004;
183(3):
455 - 467.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sieburg, A. Tripp, J.-W. Ma, and G. Feuer
Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2 Tax Oncoproteins Modulate Cell Cycle Progression and Apoptosis
J. Virol.,
October 1, 2004;
78(19):
10399 - 10409.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Aerbajinai, Y. T. Lee, U. Wojda, V. A. Barr, and J. L. Miller
Cloning and Characterization of a Gene Expressed during Terminal Differentiation That Encodes a Novel Inhibitor of Growth
J. Biol. Chem.,
January 16, 2004;
279(3):
1916 - 1921.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Roman-Gomez, J. A. Castillejo, A. Jimenez, M. G. Gonzalez, F. Moreno, M. d. C. Rodriguez, M. Barrios, J. Maldonado, and A. Torres
5' CpG island hypermethylation is associated with transcriptional silencing of the p21CIP1/WAF1/SDI1 gene and confers poor prognosis in acute lymphoblastic leukemia
Blood,
April 1, 2002;
99(7):
2291 - 2296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Dimberg, F. Bahram, I. Karlberg, L.-G. Larsson, K. Nilsson, and F. Oberg
Retinoic acid-induced cell cycle arrest of human myeloid cell lines is associated with sequential down-regulation of c-Myc and cyclin E and posttranscriptional up-regulation of p27Kip1
Blood,
March 15, 2002;
99(6):
2199 - 2206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Cheng, H. Shen, N. Rodrigues, S. Stier, and D. T. Scadden
Transforming growth factor beta 1 mediates cell-cycle arrest of primitive hematopoietic cells independent of p21Cip1/Waf1 or p27Kip1
Blood,
December 15, 2001;
98(13):
3643 - 3649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Baccini, L. Roy, N. Vitrat, H. Chagraoui, S. Sabri, J.-P. L. Couedic, N. Debili, F. Wendling, and W. Vainchenker
Role of p21Cip1/Waf1 in cell-cycle exit of endomitotic megakaryocytes
Blood,
December 1, 2001;
98(12):
3274 - 3282.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Rooney
Cell Cycle Attenuation by p120E4F Is Accompanied by Increased Mitotic Dysfunction
Cell Growth Differ.,
October 1, 2001;
12(10):
505 - 516.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Kastner, H. J. Lawrence, C. Waltzinger, N. B. Ghyselinck, P. Chambon, and S. Chan
Positive and negative regulation of granulopoiesis by endogenous RAR{alpha}
Blood,
March 1, 2001;
97(5):
1314 - 1320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Cheng, N. Rodrigues, H. Shen, Y. Yang, D. Dombkowski, M. Sykes, and D. T. Scadden
Hematopoietic Stem Cell Quiescence Maintained by p21cip1/waf1
Science,
March 10, 2000;
287(5459):
1804 - 1808.
[Abstract]
[Full Text]
|
|
|
|
|
