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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by van Egmond, M. Articles by van de Winkel, J. G.J. Search for Related Content PubMed PubMed Citation Articles by van Egmond, M. Articles by van de Winkel, J. G.J. Related Collections Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 93 No. 12 (June 15), 1999: pp. 4387-4394 Human Immunoglobulin A Receptor (FcRI, CD89) Function in Transgenic Mice Requires Both FcR  Chain and CR3 (CD11b/CD18) Marjolein van Egmond, A.J. Hanneke van Vuuren, H. Craig Morton, Annemiek B. van Spriel, Li Shen, Frans M.A. Hofhuis, Takashi Saito, Tanya N. Mayadas, J. Sjef Verbeek, and Jan G.J. van de Winkel From the Department of Immunology and Medarex Europe, University Medical Center Utrecht, Utrecht, The Netherlands; the Department of Immunohistochemistry and Immunopathology, University of Oslo, Oslo, Norway; the Department of Immunology and Microbiology, Dartmouth Medical School, Lebanon, NH; the Department of Molecular Genetics, Chiba University Graduate School of Medicine, Chiba, Japan; and the Department of Pathology, Brigham and Women's Hospital, Boston, MA. Even though more immunoglobulin A (IgA) is produced in humans than all other isotypes combined, relatively little is known about receptors that bind the Fc part of IgA. The myeloid IgA receptor, FcRI (CD89), triggers various effector functions in vitro, but its in vivo role remains unclear. Here, a transgenic mouse model is described in which FcRI is expressed under its own regulatory sequences. Receptor expression and regulation by cytokines was comparable to the human situation and hFcRI can trigger phagocytosis and lysis of tumor cells. To analyze the contribution of the FcR  chain or the 2 integrin CR3 (CD11b/CD18) in FcRI biological function, FcRI transgenic mice were crossed with either FcR  chain / or CR3 / mice. In contrast to in vitro data, FcR  chain was essential for surface expression of hFcRI in vivo. Functional studies in hFcRI/ /mice were, therefore, limited. In vitro studies showed FcR  chain to be necessary for phagocytosis. Neither hFcRI expression nor phagocytosis, triggered via hFcRI, were influenced by CR3. Remarkably, the capacity to lyse tumor targets was ablated in hFcRI transgenic/ CR3/ mice, although binding of neutrophils to tumor cells was intact. This shows a previously unrecognized importance of CR3 for hFcRI-mediated antibody-dependent cellular cytotoxicity (ADCC). CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. M. Beekman, C. E. van der Poel, J. A. van der Linden, D. L. C. van den Berg, P. V. E. van den Berghe, J. G. J. van de Winkel, and J. H. W. Leusen Filamin A Stabilizes Fc{gamma}RI Surface Expression and Prevents Its Lysosomal Routing J. Immunol., March 15, 2008; 180(6): 3938 - 3945. [Abstract] [Full Text] [PDF] M. A. Otten, J. H. W. Leusen, E. Rudolph, J. A. van der Linden, R. H. J. Beelen, J. G. J. van de Winkel, and M. van Egmond FcR {gamma}-Chain Dependent Signaling in Immature Neutrophils Is Mediated by Fc{alpha}RI, but Not by Fc{gamma}RI J. Immunol., September 1, 2007; 179(5): 2918 - 2924. [Abstract] [Full Text] [PDF] M. Dechant, T. Beyer, T. Schneider-Merck, W. Weisner, M. Peipp, J. G. J. van de Winkel, and T. 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J. van de Winkel Mac-1 (CD11b/CD18) is essential for Fc receptor-mediated neutrophil cytotoxicity and immunologic synapse formation Blood, April 15, 2001; 97(8): 2478 - 2486. [Abstract] [Full Text] [PDF] B. D. Wines, C. T. Sardjono, H. M. Trist, C.-S. Lay, and P. M. Hogarth The Interaction of Fc{{alpha}}RI with IgA and Its Implications for Ligand Binding by Immunoreceptors of the Leukocyte Receptor Cluster J. Immunol., February 1, 2001; 166(3): 1781 - 1789. [Abstract] [Full Text] [PDF] F. Geissmann, P. Launay, B. Pasquier, Y. Lepelletier, M. Leborgne, A. Lehuen, N. Brousse, and R. C. Monteiro A Subset of Human Dendritic Cells Expresses IgA Fc Receptor (CD89), Which Mediates Internalization and Activation Upon Cross-Linking by IgA Complexes J. Immunol., January 1, 2001; 166(1): 346 - 352. [Abstract] [Full Text] [PDF] M A KERR Function of immunoglobulin A in immunity Gut, December 1, 2000; 47(6): 751 - 752. [Full Text] [PDF] B. Stockmeyer, M. Dechant, M. van Egmond, A. L. Tutt, K. 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