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Blood, Vol. 93 No. 12 (June 15), 1999:
pp. 4425-4435
The Expression of Human Blood Group Antigens During Erythropoiesis
in a Cell Culture System
Mark J.G. Southcott,
Michael J.A. Tanner, and
David J. Anstee
From the Department of Biochemistry, School of Medical Sciences,
University of Bristol, Bristol, UK; and the Bristol Institute for
Transfusion Sciences, Bristol, UK.
Phenotypic analysis of hematopoietic stem and progenitor cells has
been an invaluable tool in defining the biology of stem cell
populations. We use here flow cytometry to examine the expression of
human erythroid-specific surface markers during the maturation of early
committed erythroid cells derived from cord blood in vitro. The
temporal order of the expression of erythroid specific markers was as
follows: Kell glycoprotein (gp), Rh gp, Landsteiner Wiener (LW) gp,
glycophorin A (GPA), Band 3, Lutheran (Lu) gp, and Duffy (Fy) gp. The
time at which some of these markers appeared suggests possible roles
for some of these erythroid-specific polypeptides during the
differentiation of these committed progenitors. The early appearance of
Kell gp raises the possibility that it may have an important role in
the early stages of hematopoiesis or cell lineage determination. Kell
gp may also be a useful marker for the diagnosis of erythroleukemia.
The late expression of Lu gp suggests it may be involved in the
migration of erythroid precursors from the marrow. Fy gp is also
expressed late consistent with a role as a scavenger receptor for
cytokines in the bone marrow and circulation. Rh c antigen appeared
before Rh D antigen, and it is suggested that this may reflect a
reorganization of the developing erythroid cell membrane involving the
Rh polypeptides and other components, including GPA and Band 3.
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