|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 2 (January 15), 1999:
pp. 519-526
The Activity of the CCAAT-box Binding Factor NF-Y Is Modulated
Through the Regulated Expression of Its A Subunit During Monocyte
to Macrophage Differentiation: Regulation of Tissue-Specific Genes
Through a Ubiquitous Transcription Factor
Giovanna Marziali,
Edvige Perrotti,
Ramona Ilari,
Eliana M. Coccia,
Roberto Mantovani,
Ugo Testa, and
Angela Battistini
From the Departments of Virology, Hematology and Oncology,
Immunology, Istituto Superiore di Sanità, Rome, Italy; and the
Department of Genetics and Biology of Microorganisms, University of
Milano, Milano, Italy.
In this study, we analyzed the regulation of NF-Y expression during
human monocyte to macrophage maturation. NF-Y is a ubiquitous and
evolutionarily conserved transcription factor that binds specifically to the CCAAT motif present in the 5 promoter region of a wide variety
of genes. We show here that in circulating monocytes, NF-Y binding
activity is not detected on the CCAAT motif present in the promoters of
genes such as major histocompatibility complex (MHC) class II,
gp91-phox, mig, and fibronectin, whereas during macrophage
differentiation, a progressive increase in NF-Y binding activity is
observed on these promoters. Analysis of NF-Y subunit expression
indicates that the absence of NF-Y activity in circulating monocytes is
caused by a lack of the A subunit. Furthermore, addition of the
recombinant NF-YA subunit restores NF-Y binding. We show that the lack
of NF-YA protein is due to posttranscriptional regulation and not to a
specific proteolytic activity. In fact, NF-YA mRNA is present at the
same level at all days of monocyte cultivation, whereas the protein is
absent in freshly isolated monocytes but is progressively synthesized
during the maturation process. We thus conclude that the NF-YA subunit
plays a relevant role in activating transcription of genes highly
expressed in mature monocytes. In line with this conclusion, we show
that the cut/CDP protein, a transcriptional repressor that inhibits
gpc91-phox gene expression by preventing NF-Y binding to the CAAT box,
is absent in monocytes.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
W. Aerbajinai, J. Zhu, C. Kumkhaek, K. Chin, and G. P. Rodgers
SCF induces {gamma}-globin gene expression by regulating downstream transcription factor COUP-TFII
Blood,
July 2, 2009;
114(1):
187 - 194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Coppe, F. Ferrari, A. Bisognin, G. A. Danieli, S. Ferrari, S. Bicciato, and S. Bortoluzzi
Motif discovery in promoters of genes co-localized and co-expressed during myeloid cells differentiation
Nucleic Acids Res.,
February 1, 2009;
37(2):
533 - 549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. van Wageningen, G. Nikoloski, G. Vierwinden, R. Knops, B. A. van der Reijden, and J. H. Jansen
The transcription factor nuclear factor Y regulates the proliferation of myeloid progenitor cells
Haematologica,
October 1, 2008;
93(10):
1580 - 1582.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kotecha, J. Kluza, G. Wells, C. C. O'Hare, C. Forni, R. Mantovani, P. W. Howard, P. Morris, D. E. Thurston, J. A. Hartley, et al.
Inhibition of DNA binding of the NF-Y transcription factor by the pyrrolobenzodiazepine-polyamide conjugate GWL-78
Mol. Cancer Ther.,
May 1, 2008;
7(5):
1319 - 1328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhang, B. Chen, Y. Li, J. Chen, G. Lou, M. Chen, and D. Zhou
Transcriptional Regulation of the Human PNRC Promoter by NFY in HepG2 Cells
J. Biochem.,
May 1, 2008;
143(5):
675 - 683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Benatti, V. Basile, D. Merico, L. I. Fantoni, E. Tagliafico, and C. Imbriano
A balance between NF-Y and p53 governs the pro- and anti-apoptotic transcriptional response
Nucleic Acids Res.,
March 1, 2008;
36(5):
1415 - 1428.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Borghini, M. Vargiolu, M. Di Duca, R. Ravazzolo, and I. Ceccherini
Nuclear Factor Y Drives Basal Transcription of the Human TLX3, a Gene Overexpressed in T-Cell Acute Lymphocytic Leukemia
Mol. Cancer Res.,
September 1, 2006;
4(9):
635 - 643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-Y. Kam, K.-H. Jeong, E. R. Norwitz, E. M. Jorgensen, and U. B. Kaiser
Oct-1 and Nuclear Factor Y Bind to the SURG-1 Element to Direct Basal and Gonadotropin-Releasing Hormone (GnRH)-Stimulated Mouse GnRH Receptor Gene Transcription
Mol. Endocrinol.,
January 1, 2005;
19(1):
148 - 162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Deng, G.-A. Song, E. Pong, M. Sleisenger, and Y. S. Kim
Promoter Methylation Inhibits APC Gene Expression by Causing Changes in Chromatin Conformation and Interfering with the Binding of Transcription Factor CCAAT-Binding Factor
Cancer Res.,
April 15, 2004;
64(8):
2692 - 2698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhu, D. M. Giannola, Y. Zhang, A. J. Rivera, and S. G. Emerson
NF-Y cooperates with USF1/2 to induce the hematopoietic expression of HOXB4
Blood,
October 1, 2003;
102(7):
2420 - 2427.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Yun, H.-D. Chae, T.-S. Choi, E.-H. Kim, Y.-J. Bang, J. Chung, K.-S. Choi, R. Mantovani, and D. Y. Shin
Cdk2-dependent Phosphorylation of the NF-Y Transcription Factor and Its Involvement in the p53-p21 Signaling Pathway
J. Biol. Chem.,
September 19, 2003;
278(38):
36966 - 36972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Scheef, N. Fischer, E. Flory, I. Schmitt, and R. R. Tonjes
Transcriptional Regulation of Porcine Endogenous Retroviruses Released from Porcine and Infected Human Cells by Heterotrimeric Protein Complex NF-Y and Impact of Immunosuppressive Drugs
J. Virol.,
November 13, 2002;
76(24):
12553 - 12563.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hackzell, H. Uramoto, H. Izumi, K. Kohno, and K. Funa
p73 Independent of c-Myc Represses Transcription of Platelet-derived Growth Factor beta -Receptor through Interaction with NF-Y
J. Biol. Chem.,
October 11, 2002;
277(42):
39769 - 39776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Tjin Tham Sjin, K. Krishnaraju, B. Hoffman, and D. A. Liebermann
Transcriptional regulation of myeloid differentiation primary response (MyD) genes during myeloid differentiation is mediated by nuclear factor Y
Blood,
June 17, 2002;
100(1):
80 - 88.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Peng and N. Jahroudi
The NFY transcription factor functions as a repressor and activator of the von Willebrand factor promoter
Blood,
April 1, 2002;
99(7):
2408 - 2417.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kumatori, D. Yang, S. Suzuki, and M. Nakamura
Cooperation of STAT-1 and IRF-1 in Interferon-gamma -induced Transcription of the gp91phox Gene
J. Biol. Chem.,
March 8, 2002;
277(11):
9103 - 9111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H Izumi, C Molander, L. Penn, A Ishisaki, K Kohno, and K Funa
Mechanism for the transcriptional repression by c-Myc on PDGF (&bgr;)-receptor
J. Cell Sci.,
January 4, 2001;
114(8):
1533 - 1544.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G. Caretti, M. C. Motta, and R. Mantovani
NF-Y Associates with H3-H4 Tetramers and Octamers by Multiple Mechanisms
Mol. Cell. Biol.,
December 1, 1999;
19(12):
8591 - 8603.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. S. Radomska, A. B. Satterthwaite, N. Taranenko, S. Narravula, D. S. Krause, and D. G. Tenen
A Nuclear Factor Y (NFY) Site Positively Regulates the Human CD34 Stem Cell Gene
Blood,
December 1, 1999;
94(11):
3772 - 3780.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Inoue, J. Kamiyama, and T. Sakai
Sp1 and NF-Y Synergistically Mediate the Effect of Vitamin D3 in the p27Kip1 Gene Promoter That Lacks Vitamin D Response Elements
J. Biol. Chem.,
November 5, 1999;
274(45):
32309 - 32317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. Snyder, J. Wang, J. F. Waring, and G. D. Ginder
Identification of CCAAT Displacement Protein (CDP/cut) as a Locus-specific Repressor of Major Histocompatibility Complex Gene Expression in Human Tumor Cells
J. Biol. Chem.,
February 9, 2001;
276(7):
5323 - 5330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
