|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 2 (January 15), 1999:
pp. 571-579
Identification of a Cellubrevin/Vesicle Associated Membrane Protein 3 Homologue in Human Platelets
Audrey M. Bernstein and
Sidney W. Whiteheart
From the Department of Biochemistry, University of Kentucky College
of Medicine, Chandler Medical Center, Lexington, KY.
Several studies suggest membrane trafficking events are mediated by
integral, membrane proteins from both transport-vesicle and target
membranes, called v- and t-SNAREs (SNAp REceptors), respectively.
Previous experiments using antibodies to synaptobrevin/vesicle associated membrane protein (VAMP) 1, 2, or rat cellubrevin failed to
detect these v-SNAREs in human platelets, although membrane proteins
from these cells could support 20S complex formation. To
identify v-SNAREs in platelets, we used a polymerase chain reaction
(PCR) approach with degenerate primers to amplify potential VAMP-like
v-SNAREs. A cDNA encoding a novel v-SNARE was isolated from a human
megakaryocyte cDNA library. Termed human cellubrevin (Hceb), this
protein has greater than 93% identity with human VAMP 1, 2, and rat
cellubrevin over the conserved core region, but has a unique
N-terminal domain. Northern blot analysis showed that the 2.5-kB mRNA
encoding Hceb is expressed in every human tissue tested. Hceb from
detergent-solubilized platelet membranes, participated in
 -SNAP-dependent 20S complex formation and adenosine triphosphate
(ATP)-dependent disassembly, showing that Hceb can act as a v-SNARE in
platelets. Immunofluorescence microscopy, using an anti-Hceb antibody
showed a punctate, intracellular staining pattern in platelets,
megakaryocytes, and HEK-293 cells. This same pattern was observed in
surface-activated platelets even though all dense core and most
-granule contents had been released. These data suggest that Hceb
may reside on a platelet organelle that is not primarily involved in
the exocytic pathway.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. Flaumenhaft, N. Rozenvayn, D. Feng, and A. M. Dvorak
SNAP-23 and syntaxin-2 localize to the extracellular surface of the platelet plasma membrane
Blood,
September 1, 2007;
110(5):
1492 - 1501.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Ren, H. K. Barber, G. L. Crawford, Z. A. Karim, C. Zhao, W. Choi, C.-C. Wang, W. Hong, and S. W. Whiteheart
Endobrevin/VAMP-8 Is the Primary v-SNARE for the Platelet Release Reaction
Mol. Biol. Cell,
January 1, 2007;
18(1):
24 - 33.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Rosado, P. C. Redondo, G. M. Salido, S. O. Sage, and J. A. Pariente
Cleavage of SNAP-25 and VAMP-2 impairs store-operated Ca2+ entry in mouse pancreatic acinar cells
Am J Physiol Cell Physiol,
January 1, 2005;
288(1):
C214 - C221.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. C. Redondo, A. G. S. Harper, G. M. Salido, J. A. Pariente, S. O. Sage, and J. A. Rosado
A role for SNAP-25 but not VAMPs in store-mediated Ca2+ entry in human platelets
J. Physiol.,
July 1, 2004;
558(1):
99 - 109.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Randriamboavonjy, J. Schrader, R. Busse, and I. Fleming
Insulin Induces the Release of Vasodilator Compounds From Platelets by a Nitric Oxide-G Kinase-VAMP-3-dependent Pathway
J. Exp. Med.,
February 2, 2004;
199(3):
347 - 356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Polgar, W. S. Lane, S.-H. Chung, A. K. Houng, and G. L. Reed
Phosphorylation of SNAP-23 in Activated Human Platelets
J. Biol. Chem.,
November 7, 2003;
278(45):
44369 - 44376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. D. Schraw, T. W. Rutledge, G. L. Crawford, A. M. Bernstein, A. L. Kalen, J. E. Pessin, and S. W. Whiteheart
Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release
Blood,
September 1, 2003;
102(5):
1716 - 1722.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Flaumenhaft
Molecular Basis of Platelet Granule Secretion
Arterioscler. Thromb. Vasc. Biol.,
July 1, 2003;
23(7):
1152 - 1160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Rozenvayn and R. Flaumenhaft
Protein kinase C Mediates Translocation of Type II Phosphatidylinositol 5-Phosphate 4-Kinase Required for Platelet alpha -Granule Secretion
J. Biol. Chem.,
February 28, 2003;
278(10):
8126 - 8134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Polgar, S.-H. Chung, and G. L. Reed
Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion
Blood,
July 18, 2002;
100(3):
1081 - 1083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Feng, K. Crane, N. Rozenvayn, A. M. Dvorak, and R. Flaumenhaft
Subcellular distribution of 3 functional platelet SNARE proteins: human cellubrevin, SNAP-23, and syntaxin 2
Blood,
May 13, 2002;
99(11):
4006 - 4014.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. L. Reed, M. L. Fitzgerald, and J. Polgar
Molecular mechanisms of platelet exocytosis: insights into the "secrete" life of thrombocytes
Blood,
November 15, 2000;
96(10):
3334 - 3342.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Chen, P. P. Lemons, T. Schraw, and S. W. Whiteheart
Molecular mechanisms of platelet exocytosis: role of SNAP-23 and syntaxin 2 and 4 in lysosome release
Blood,
September 1, 2000;
96(5):
1782 - 1788.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Chen, A. M. Bernstein, P. P. Lemons, and S. W. Whiteheart
Molecular mechanisms of platelet exocytosis: role of SNAP-23 and syntaxin 2 in dense core granule release
Blood,
February 1, 2000;
95(3):
921 - 929.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Rozenvayn and R. Flaumenhaft
Phosphatidylinositol 4,5-Bisphosphate Mediates Ca2+-induced Platelet alpha -Granule Secretion. EVIDENCE FOR TYPE II PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE FUNCTION
J. Biol. Chem.,
June 15, 2001;
276(25):
22410 - 22419.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
