|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 3 (February 1), 1999:
pp. 857-865
The MKK6/p38 Stress Kinase Cascade Is Critical for Tumor Necrosis
Factor- -Induced Expression of Monocyte-Chemoattractant
Protein-1 in Endothelial Cells
Matthias Goebeler,
Karin Kilian,
Reinhard Gillitzer,
Manfred Kunz,
Teizo Yoshimura,
Eva-B. Bröcker,
Ulf R. Rapp, and
Stephan Ludwig
From the Klinik und Poliklinik für Haut- und
Geschlechtskrankheiten and Institut für Medizinische
Strahlenkunde und Zellforschung (MSZ), Universität
Würzburg, Würzburg, Germany; and the Immunopathology
Section, Laboratory of Immunobiology, National Cancer
Institute-Frederick, Frederick, MD.
Monocyte chemoattractant protein-1 (MCP-1), a member of the C-C
subfamily of chemokines, is important for the local recruitment of
leukocytes to sites of inflammatory challenge. Here, we investigated endothelial signaling pathways involving members of the
mitogen-activated protein (MAP) kinase superfamily and studied their
role for MCP-1 expression in endothelium. We show that tumor necrosis
factor- (TNF-), a potent inflammatory activator of endothelium,
leads to activation of MAP kinases ERK, p38, and JNK in human umbilical vein endothelial cells (HUVEC). Contribution of MAP kinase pathways to
TNF--induced synthesis of endothelial MCP-1 was then studied by
pharmacologic inhibition and transient expression of dominant negative
or constitutively active kinase mutants using flow cytometry, Northern
blot, and luciferase reporter gene assays. Inhibition of Raf/MEK/ERK or
SEK/JNK pathways had no significant effect on MCP-1 levels, whereas
blocking the MKK6/p38 pathway by p38 inhibitors SB203580 or SB202190 or
by a dominant negative mutant of MKK6, the upstream activator of p38,
strongly inhibited TNF--induced expression of MCP-1. Consistent
with that finding, expression of wild-type or constitutively active
MKK6 significantly enhanced the effect of limiting TNF-
concentrations on MCP-1 synthesis. These data suggest a crucial role
for the MKK6/p38 stress kinase cascade in TNF--mediated endothelial
MCP-1 expression.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G Schett, J Zwerina, and G Firestein
The p38 mitogen-activated protein kinase (MAPK) pathway in rheumatoid arthritis
Ann Rheum Dis,
July 1, 2008;
67(7):
909 - 916.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Takahashi, E. Suzuki, R. Takeda, S. Oba, H. Nishimatsu, K. Kimura, T. Nagano, R. Nagai, and Y. Hirata
Angiotensin II and tumor necrosis factor-{alpha} synergistically promote monocyte chemoattractant protein-1 expression: roles of NF-{kappa}B, p38, and reactive oxygen species
Am J Physiol Heart Circ Physiol,
June 1, 2008;
294(6):
H2879 - H2888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Chen, C. S. K. Kam, F. Q. Liu, Y. Liu, V. C. H. Lui, J. R. Lamb, and P. K. H. Tam
LPS-induced up-regulation of TGF-{beta} receptor 1 is associated with TNF-{alpha} expression in human monocyte-derived macrophages
J. Leukoc. Biol.,
May 1, 2008;
83(5):
1165 - 1173.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Stone, G. L. Kolling, M. H. Lindner, and T. G. Obrig
p38 Mitogen-Activated Protein Kinase Mediates Lipopolysaccharide and Tumor Necrosis Factor Alpha Induction of Shiga Toxin 2 Sensitivity in Human Umbilical Vein Endothelial Cells
Infect. Immun.,
March 1, 2008;
76(3):
1115 - 1121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Muller, D. Viemann, M. Schmidt, N. Endres, S. Ludwig, M. Leverkus, J. Roth, and M. Goebeler
Candida albicans Triggers Activation of Distinct Signaling Pathways to Establish a Proinflammatory Gene Expression Program in Primary Human Endothelial Cells
J. Immunol.,
December 15, 2007;
179(12):
8435 - 8445.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yoshimura and M. Takahashi
IFN-{gamma}-Mediated Survival Enables Human Neutrophils to Produce MCP-1/CCL2 in Response to Activation by TLR Ligands
J. Immunol.,
August 1, 2007;
179(3):
1942 - 1949.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Gorska, Q. Liang, S. J. Stafford, N. Goplen, N. Dharajiya, L. Guo, S. Sur, M. Gaestel, and R. Alam
MK2 controls the level of negative feedback in the NF-{kappa}B pathway and is essential for vascular permeability and airway inflammation
J. Exp. Med.,
July 9, 2007;
204(7):
1637 - 1652.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Viemann, M. Schmidt, K. Tenbrock, S. Schmid, V. Muller, K. Klimmek, S. Ludwig, J. Roth, and M. Goebeler
The Contact Allergen Nickel Triggers a Unique Inflammatory and Proangiogenic Gene Expression Pattern via Activation of NF-{kappa}B and Hypoxia-Inducible Factor-1{alpha}
J. Immunol.,
March 1, 2007;
178(5):
3198 - 3207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. K. R. Karaolis, T. K. Means, D. Yang, M. Takahashi, T. Yoshimura, E. Muraille, D. Philpott, J. T. Schroeder, M. Hyodo, Y. Hayakawa, et al.
Bacterial c-di-GMP Is an Immunostimulatory Molecule
J. Immunol.,
February 15, 2007;
178(4):
2171 - 2181.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Seval, H. Cakmak, U. A. Kayisli, and A. Arici
Estrogen-Mediated Regulation of p38 Mitogen-Activated Protein Kinase in Human Endometrium
J. Clin. Endocrinol. Metab.,
June 1, 2006;
91(6):
2349 - 2357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Niwano, M. Arai, N. Koitabashi, S. Hara, A. Watanabe, K. Sekiguchi, T. Tanaka, T. Iso, and M. Kurabayashi
Competitive Binding of CREB and ATF2 to cAMP/ATF Responsive Element Regulates eNOS Gene Expression in Endothelial Cells
Arterioscler. Thromb. Vasc. Biol.,
May 1, 2006;
26(5):
1036 - 1042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Hoyt, J. Ballering, H. Numanami, J. M. Hayden, and R. A. Robbins
Doxycycline Modulates Nitric Oxide Production in Murine Lung Epithelial Cells
J. Immunol.,
January 1, 2006;
176(1):
567 - 572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Kuldo, J. Westra, S. A. Asgeirsdottir, R. J. Kok, K. Oosterhuis, M. G. Rots, J. P. Schouten, P. C. Limburg, and G. Molema
Differential effects of NF-{kappa}B and p38 MAPK inhibitors and combinations thereof on TNF-{alpha}- and IL-1{beta}-induced proinflammatory status of endothelial cells in vitro
Am J Physiol Cell Physiol,
November 1, 2005;
289(5):
C1229 - C1239.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Thiefes, S. Wolter, J. F. Mushinski, E. Hoffmann, O. Dittrich-Breiholz, N. Graue, A. Dorrie, H. Schneider, D. Wirth, B. Luckow, et al.
Simultaneous Blockade of NF{kappa}B, JNK, and p38 MAPK by a Kinase-inactive Mutant of the Protein Kinase TAK1 Sensitizes Cells to Apoptosis and Affects a Distinct Spectrum of Tumor Necrosis Target Genes
J. Biol. Chem.,
July 29, 2005;
280(30):
27728 - 27741.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Seybold, D. Thomas, M. Witzenrath, S. Boral, A. C. Hocke, A. Burger, A. Hatzelmann, H. Tenor, C. Schudt, M. Krull, et al.
Tumor necrosis factor-{alpha}-dependent expression of phosphodiesterase 2: role in endothelial hyperpermeability
Blood,
May 1, 2005;
105(9):
3569 - 3576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Simoncini, C. Sapet, L. Camoin-Jau, N. Bardin, J.-R. Harle, J. Sampol, F. Dignat-George, and F. Anfosso
Role of reactive oxygen species and p38 MAPK in the induction of the pro-adhesive endothelial state mediated by IgG from patients with anti-phospholipid syndrome
Int. Immunol.,
April 1, 2005;
17(4):
489 - 500.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-L. Chen, J. Y. Grey, S. Thomas, F.-H. Qiu, R. M. Medford, M. A. Wasserman, and C. Kunsch
Sphingosine kinase-1 mediates TNF-{alpha}-induced MCP-1 gene expression in endothelial cells: upregulation by oscillatory flow
Am J Physiol Heart Circ Physiol,
October 1, 2004;
287(4):
H1452 - H1458.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. J. Wurzer, C. Ehrhardt, S. Pleschka, F. Berberich-Siebelt, T. Wolff, H. Walczak, O. Planz, and S. Ludwig
NF-{kappa}B-dependent Induction of Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL) and Fas/FasL Is Crucial for Efficient Influenza Virus Propagation
J. Biol. Chem.,
July 23, 2004;
279(30):
30931 - 30937.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Suzuki, H. Satonaka, H. Nishimatsu, S. Oba, R. Takeda, M. Omata, T. Fujita, R. Nagai, and Y. Hirata
Myocyte Enhancer Factor 2 Mediates Vascular Inflammation via the p38-Dependent Pathway
Circ. Res.,
July 9, 2004;
95(1):
42 - 49.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-M. Chen, W.-C. Chiang, S.-L. Lin, K.-D. Wu, T.-J. Tsai, and B.-S. Hsieh
Dual Regulation of Tumor Necrosis Factor-{alpha}-Induced CCL2/Monocyte Chemoattractant Protein-1 Expression in Vascular Smooth Muscle Cells by Nuclear Factor-{kappa}B and Activator Protein-1: Modulation by Type III Phosphodiesterase Inhibition
J. Pharmacol. Exp. Ther.,
June 1, 2004;
309(3):
978 - 986.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Viemann, M. Goebeler, S. Schmid, K. Klimmek, C. Sorg, S. Ludwig, and J. Roth
Transcriptional profiling of IKK2/NF-{kappa}B-- and p38 MAP kinasedependent gene expression in TNF-{alpha}--stimulated primary human endothelial cells
Blood,
May 1, 2004;
103(9):
3365 - 3373.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Hou, M. Eren, C. A. Painter, J. W. Covington, J. D. Dixon, J. A. Schoenhard, and D. E. Vaughan
Tumor Necrosis Factor {alpha} Activates the Human Plasminogen Activator Inhibitor-1 Gene through a Distal Nuclear Factor {kappa}B Site
J. Biol. Chem.,
April 30, 2004;
279(18):
18127 - 18136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Satonaka, E. Suzuki, H. Nishimatsu, S. Oba, R. Takeda, A. Goto, M. Omata, T. Fujita, R. Nagai, and Y. Hirata
Calcineurin Promotes the Expression of Monocyte Chemoattractant Protein-1 in Vascular Myocytes and Mediates Vascular Inflammation
Circ. Res.,
March 19, 2004;
94(5):
693 - 700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Matsuyama, L. Wang, W. L. Farrar, M. Faure, and T. Yoshimura
Activation of Discoidin Domain Receptor 1 Isoform b with Collagen Up-Regulates Chemokine Production in Human Macrophages: Role of p38 Mitogen-Activated Protein Kinase and NF-{kappa}B
J. Immunol.,
February 15, 2004;
172(4):
2332 - 2340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Baronas-Lowell, J. L. Lauer-Fields, and G. B. Fields
Induction of Endothelial Cell Activation by a Triple Helical {alpha}2{beta}1 Integrin Ligand, Derived from Type I Collagen {alpha}1(I)496-507
J. Biol. Chem.,
January 9, 2004;
279(2):
952 - 962.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Gertzberg, P. Neumann, V. Rizzo, and A. Johnson
NAD(P)H oxidase mediates the endothelial barrier dysfunction induced by TNF-{alpha}
Am J Physiol Lung Cell Mol Physiol,
January 1, 2004;
286(1):
L37 - L48.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kawaguchi, F. Kokubu, S. Matsukura, K. Ieki, M. Odaka, S. Watanabe, S. Suzuki, M. Adachi, and S.-K. Huang
Induction of C-X-C Chemokines, Growth-Related Oncogene {alpha} Expression, and Epithelial Cell-Derived Neutrophil-Activating Protein-78 by ML-1 (Interleukin-17F) Involves Activation of Raf1-Mitogen-Activated Protein Kinase Kinase-Extracellular Signal-Regulated Kinase 1/2 Pathway
J. Pharmacol. Exp. Ther.,
December 1, 2003;
307(3):
1213 - 1220.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Donadelli, C. Zanchi, M. Morigi, S. Buelli, C. Batani, S. Tomasoni, D. Corna, D. Rottoli, A. Benigni, M. Abbate, et al.
Protein Overload Induces Fractalkine Upregulation in Proximal Tubular Cells through Nuclear Factor {kappa}B- and p38 Mitogen-Activated Protein Kinase-Dependent Pathways
J. Am. Soc. Nephrol.,
October 1, 2003;
14(10):
2436 - 2446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Takaya, D. Koya, M. Isono, T. Sugimoto, T. Sugaya, A. Kashiwagi, and M. Haneda
Involvement of ERK pathway in albumin-induced MCP-1 expression in mouse proximal tubular cells
Am J Physiol Renal Physiol,
May 1, 2003;
284(5):
F1037 - F1045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kaur, R. C. Woodman, and P. Kubes
P38 MAPK: critical molecule in thrombin-induced NF-kappa B-dependent leukocyte recruitment
Am J Physiol Heart Circ Physiol,
April 1, 2003;
284(4):
H1095 - H1103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Fiuza, M. Bustin, S. Talwar, M. Tropea, E. Gerstenberger, J. H. Shelhamer, and A. F. Suffredini
Inflammation-promoting activity of HMGB1 on human microvascular endothelial cells
Blood,
April 1, 2003;
101(7):
2652 - 2660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Iwata, T. Wada, K. Furuichi, N. Sakai, K. Matsushima, H. Yokoyama, and K.-i. Kobayashi
p38 Mitogen-Activated Protein Kinase Contributes to Autoimmune Renal Injury in MRL-Faslpr Mice
J. Am. Soc. Nephrol.,
January 1, 2003;
14(1):
57 - 67.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Nick, S. K. Young, P. G. Arndt, J. G. Lieber, B. T. Suratt, K. R. Poch, N. J. Avdi, K. C. Malcolm, C. Taube, P. M. Henson, et al.
Selective Suppression of Neutrophil Accumulation in Ongoing Pulmonary Inflammation by Systemic Inhibition of p38 Mitogen-Activated Protein Kinase
J. Immunol.,
November 1, 2002;
169(9):
5260 - 5269.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tanaka, T. Kuroiwa, H. Ikeuchi, F. Ota, Y. Kaneko, K. Ueki, Y. Tsukada, I. B. McInnes, D. T. Boumpas, and Y. Nojima
Human Platelets Stimulate Mesangial Cells to Produce Monocyte Chemoattractant Protein-1 via the CD40/CD40 Ligand Pathway and May Amplify Glomerular Injury
J. Am. Soc. Nephrol.,
October 1, 2002;
13(10):
2488 - 2496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Takanami-Ohnishi, S. Amano, S. Kimura, S. Asada, A. Utani, M. Maruyama, H. Osada, H. Tsunoda, Y. Irukayama-Tomobe, K. Goto, et al.
Essential Role of p38 Mitogen-activated Protein Kinase in Contact Hypersensitivity
J. Biol. Chem.,
September 27, 2002;
277(40):
37896 - 37903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N.-H. Cho, S.-Y. Seong, M.-S. Huh, N.-H. Kim, M.-s. Choi, and I.-s. Kim
Induction of the Gene Encoding Macrophage Chemoattractant Protein 1 by Orientia tsutsugamushi in Human Endothelial Cells Involves Activation of Transcription Factor Activator Protein 1
Infect. Immun.,
September 1, 2002;
70(9):
4841 - 4850.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kawaguchi, L. F. Onuchic, and S.-K. Huang
Activation of Extracellular Signal-regulated Kinase (ERK)1/2, but Not p38 and c-Jun N-terminal Kinase, Is Involved in Signaling of a Novel Cytokine, ML-1
J. Biol. Chem.,
May 3, 2002;
277(18):
15229 - 15232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Furuichi, T. Wada, Y. Iwata, N. Sakai, K. Yoshimoto, K.-i. Kobayashi, N. Mukaida, K. Matsushima, and H. Yokoyama
Administration of FR167653, a new anti-inflammatory compound, prevents renal ischaemia/reperfusion injury in mice
Nephrol. Dial. Transplant.,
March 1, 2002;
17(3):
399 - 407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Marin, C. Farnarier, S. Gres, S. Kaplanski, M. S.-S. Su, C. A. Dinarello, and G. Kaplanski
The p38 mitogen-activated protein kinase pathway plays a critical role in thrombin-induced endothelial chemokine production and leukocyte recruitment
Blood,
August 1, 2001;
98(3):
667 - 673.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-M. Bian, V. M. Elner, A. Yoshida, S. L. Kunkel, and S. G. Elner
Signaling Pathways for Glycated Human Serum Albumin-Induced IL-8 and MCP-1 Secretion in Human RPE Cells
Invest. Ophthalmol. Vis. Sci.,
June 1, 2001;
42(7):
1660 - 1668.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. T. Maizels, A. Mukherjee, G. Sithanandam, C. A. Peters, J. Cottom, K. E. Mayo, and M. Hunzicker-Dunn
Developmental Regulation of Mitogen-Activated Protein Kinase-Activated Kinases-2 and -3 (MAPKAPK-2/-3) in Vivo during Corpus Luteum Formation in the Rat
Mol. Endocrinol.,
May 1, 2001;
15(5):
716 - 733.
[Abstract]
[Full Text]
|
|
|
|
