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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tozer, E. C. Articles by Loftus, J. C. Search for Related Content PubMed PubMed Citation Articles by Tozer, E. C. Articles by Loftus, J. C. Related Collections Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 93 No. 3 (February 1), 1999: pp. 918-924 A Mutation in the Subunit of the Platelet Integrin IIb3 Identifies a Novel Region Important for Ligand Binding Eileen Collins Tozer, Elizabeth K. Baker, Mark H. Ginsberg, and Joseph C. Loftus From the Department of Vascular Biology, The Scripps Research Institute, La Jolla, CA. An unbiased genetic approach was used to identify a specific amino acid residue in the IIb subunit important for the ligand binding function of the integrin IIb. Chemically mutagenized cells were selected by flow cytometry based on their inability to bind the ligand mimetic antibody PAC1 and a cell line containing a single amino acid substitution in IIb at position 224 (DV) was identified. Although well expressed on the surface of transfected cells, IIbD224V3 as well as IIbD224A3 did not bind IIb3-specific ligands or a RGD peptide, a ligand shared in common with v3. Insertion of exon 5 of IIb, residues G193-W235, into the backbone of the v subunit did not enable the chimeric receptor to bind IIb3-specific ligands. However, the chimeric receptor was still capable of binding to a RGD affinity matrix. IIbD224 is not well conserved among other integrin subunits and is located in a region of significant variability. In addition, amino acid D224 lies within a predicted loop of the recently proposed -propeller model for integrin subunits and is adjacent to a loop containing amino acid residues previously implicated in receptor function. These data support a role for this region in ligand binding function of the IIb3 receptor. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. P. Podolnikova, V. P. Yakubenko, G. L. Volkov, E. F. Plow, and T. P. Ugarova Identification of a Novel Binding Site for Platelet Integrins {alpha}IIb{beta}3 (GPIIbIIIa) and {alpha}5{beta}1 in the {gamma}C-domain of Fibrinogen J. Biol. Chem., August 22, 2003; 278(34): 32251 - 32258. [Abstract] [Full Text] [PDF] T. Kamata, K. K. Tieu, A. Irie, T. A. Springer, and Y. 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