|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 4 (February 15), 1999:
pp. 1145-1156
Coreceptor/Chemokine Receptor Expression on Human Hematopoietic
Cells: Biological Implications for Human Immunodeficiency Virus-Type
1 Infection
Benhur Lee,
Janina Ratajczak,
Robert W. Doms,
Alan M. Gewirtz, and
Mariusz Z. Ratajczak
From the Department of Medicine and the Department of Pathology and
Laboratory Medicine, Hospital of the University of Pennsylvania,
Philadelphia, PA.
The recent discovery of chemokine receptors as coreceptors for human
immunodeficiency virus-type 1 (HIV-1) entry offers new avenues for investigating the pathogenesis of acquired immunodeficiency syndrome (AIDS)-related cytopenias. To this end, we sought to (1)
phenotype human hematopoietic cells for CD4 and the HIV-1 coreceptors
CXCR4, CCR5, CCR3, and CCR2b; (2) correlate CD4 and chemokine receptor
expression with their susceptibility to HIV-1 infection; and
(3) examine any potential interplay between inflammatory cytokines
released during HIV-1 infection and regulation of chemokine receptor
expression. Fluorescence-activated cell sorting (FACS) analysis of bone
marrow mononuclear cells (BMMNC), cells derived from serum-free
expanded hematopoietic lineages (colony-forming unit-granulocyte-macrophage [CFU-GM], colony-forming
unit-megakaryocyte [CFU-Meg], and burst-forming unit-erythroid
[BFU-E]), and CD34+ cells showed
differential expression of chemokine receptors and CD4 with some
lineage specificity. Significantly, FACS-sorted CXCR4+/CD34+ cells had the same clonogeneic
potential as CXCR4 /CD34+ cells. Reverse
transcriptase-polymerase chain reaction (RT-PCR) analysis of
FACS-sorted human candidate stem cells (HSC; CD34+,
c-kit+, Rho123low) showed the presence of
CXCR4 mRNA but not CD4 mRNA. Infection studies with HIV-1
Env-pseudotyped luciferase reporter viruses indicated that X4 Env
(CXCR4-using) pseudotypes infected megakaryocytic cells, whereas R5 Env
(CCR5-using) pseudotypes did not. Similarly, R5 but not X4
Env-pseudotyped viruses infected granulocyte-macrophage cells in a
CD4/CCR5-dependent manner. Erythroid cells were resistant to R5 or X4
viral infection. Finally, we found that  -interferon treatment
upregulated CXCR4 expression on primary hematopoietic cells. In
summary, the delineation of chemokine receptor expression on primary
hematopoietic cells is a first step towards dissecting the
chemokine-chemokine receptor axes that may play a role in hematopoietic
cell proliferation and homing. Furthermore, susceptibility of
hematopoietic cells to HIV-1 infection is likely to be more complicated
than the mere physical presence of CD4 and the cognate chemokine
receptor. Lastly, our results suggest a potential interplay between
-interferon secretion and CXCR4 expression.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. J. Chancey, K. V. Khanna, J. F. M. L. Seegers, G. W. Zhang, J. Hildreth, A. Langan, and R. B. Markham
Lactobacilli-Expressed Single-Chain Variable Fragment (scFv) Specific for Intercellular Adhesion Molecule 1 (ICAM-1) Blocks Cell-Associated HIV-1 Transmission across a Cervical Epithelial Monolayer
J. Immunol.,
May 1, 2006;
176(9):
5627 - 5636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Reca, D. Mastellos, M. Majka, L. Marquez, J. Ratajczak, S. Franchini, A. Glodek, M. Honczarenko, L. A. Spruce, A. Janowska-Wieczorek, et al.
Functional receptor for C3a anaphylatoxin is expressed by normal hematopoietic stem/progenitor cells, and C3a enhances their homing-related responses to SDF-1
Blood,
May 15, 2003;
101(10):
3784 - 3793.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Tomita, G. Caramori, S. Lim, K. Ito, T. Hanazawa, T. Oates, I. Chiselita, E. Jazrawi, K. F. Chung, P. J. Barnes, et al.
Increased p21CIP1/WAF1 and B Cell Lymphoma Leukemia-xL Expression and Reduced Apoptosis in Alveolar Macrophages from Smokers
Am. J. Respir. Crit. Care Med.,
September 1, 2002;
166(5):
724 - 731.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Youssefian, A. Drouin, J.-M. Masse, J. Guichard, and E. M. Cramer
Host defense role of platelets: engulfment of HIV and Staphylococcus aureus occurs in a specific subcellular compartment and is enhanced by platelet activation
Blood,
May 13, 2002;
99(11):
4021 - 4029.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Munch, L. Standker, S. Pohlmann, F. Baribaud, A. Papkalla, O. Rosorius, R. Stauber, G. Sass, N. Heveker, K. Adermann, et al.
Hemofiltrate CC Chemokine 1[9-74] Causes Effective Internalization of CCR5 and Is a Potent Inhibitor of R5-Tropic Human Immunodeficiency Virus Type 1 Strains in Primary T Cells and Macrophages
Antimicrob. Agents Chemother.,
April 1, 2002;
46(4):
982 - 990.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kijowski, M. Baj-Krzyworzeka, M. Majka, R. Reca, L. A. Marquez, M. Christofidou-Solomidou, A. Janowska-Wieczorek, and M. Z. Ratajczak
The SDF-1-CXCR4 Axis Stimulates VEGF Secretion and Activates Integrins but does not Affect Proliferation and Survival in Lymphohematopoietic Cells
Stem Cells,
September 1, 2001;
19(5):
453 - 466.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, L. Li, R. Wadley, S. W. Reddel, J. C. Qi, C. Archis, A. Collins, E. Clark, M. Cooley, S. Kouts, et al.
Mast cells/basophils in the peripheral blood of allergic individuals who are HIV-1 susceptible due to their surface expression of CD4 and the chemokine receptors CCR3, CCR5, and CXCR4
Blood,
June 1, 2001;
97(11):
3484 - 3490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Majka, A. Janowska-Wieczorek, J. Ratajczak, K. Ehrenman, Z. Pietrzkowski, M. A. Kowalska, A. M. Gewirtz, S. G. Emerson, and M. Z. Ratajczak
Numerous growth factors, cytokines, and chemokines are secreted by human CD34+ cells, myeloblasts, erythroblasts, and megakaryoblasts and regulate normal hematopoiesis in an autocrine/paracrine manner
Blood,
May 15, 2001;
97(10):
3075 - 3085.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Kim, X. J. You, M.-E. Harmon, J. Overbaugh, and H. Fan
Use of Helper-Free Replication-Defective Simian Immunodeficiency Virus-Based Vectors To Study Macrophage and T Tropism: Evidence for Distinct Levels of Restriction in Primary Macrophages and a T-Cell Line
J. Virol.,
March 1, 2001;
75(5):
2288 - 2300.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Gazitt, P. Shaughnessy, and Q. Liu
Expression of Adhesion Molecules on CD34+ Cells in Peripheral Blood of Non-Hodgkin's Lymphoma Patients Mobilized with Different Growth Factors
Stem Cells,
February 1, 2001;
19(2):
134 - 143.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. Pertusini, J. Ratajczak, M. Majka, D. Vaughn, M. Z. Ratajczak, and A. M. Gewirtz
Investigating the platelet-sparing mechanism of paclitaxel/carboplatin combination chemotherapy
Blood,
February 1, 2001;
97(3):
638 - 644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Gazitt and Q. Liu
Plasma Levels of SDF-1 and Expression of SDF-1 Receptor on CD34+ Cells in Mobilized Peripheral Blood of Non-Hodgkin's Lymphoma Patients
Stem Cells,
January 1, 2001;
19(1):
37 - 45.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Majka, A. Janowska-Wieczorek, J. Ratajczak, M. A. Kowalska, G. Vilaire, Z. K. Pan, M. Honczarenko, L. A. Marquez, M. Poncz, and M. Z. Ratajczak
Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis
Blood,
December 15, 2000;
96(13):
4142 - 4151.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Blanpain, B. Lee, M. Tackoen, B. Puffer, A. Boom, F. Libert, M. Sharron, V. Wittamer, G. Vassart, R. W. Doms, et al.
Multiple nonfunctional alleles of CCR5 are frequent in various human populations
Blood,
September 1, 2000;
96(5):
1638 - 1645.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Majka, J. Ratajczak, B. Lee, M. Honczarenko, R. Douglas, M. A. Kowalska, L. Silberstein, A. M. Gewirtz, and M. Z. Ratajczak
The Role of HIV-Related Chemokine Receptors and Chemokines in Human Erythropoiesis in Vitro
Stem Cells,
March 1, 2000;
18(2):
128 - 138.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Chelucci, I. Casella, M. Federico, U. Testa, G. Macioce, E. Pelosi, R. Guerriero, G. Mariani, A. Giampaolo, H.J. Hassan, et al.
Lineage-Specific Expression of Human Immunodeficiency Virus (HIV) Receptor/Coreceptors in Differentiating Hematopoietic Precursors: Correlation With Susceptibility to T- and M-Tropic HIV and Chemokine-Mediated HIV Resistance
Blood,
September 1, 1999;
94(5):
1590 - 1600.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
