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Blood, Vol. 93 No. 4 (February 15), 1999:
pp. 1178-1188
Lineage-Restricted Expression of Protein Kinase C Isoforms in
Hematopoiesis
Alessandra Bassini,
Giorgio Zauli,
Giovanni Migliaccio,
Anna Rita Migliaccio,
Massimiliano Pascuccio,
Sabina Pierpaoli,
Lia Guidotti,
Silvano Capitani, and
Marco Vitale
From the Institute of Histology and General Embryology, University of
Bologna, Bologna, Italy; the Human Anatomy Section, Department of
Morphology and Embryology, University of Ferrara, Ferrara, Italy; the
Laboratory of Cell Biology, Istituto Superiore di Sanità, Roma,
Italy; the Human Anatomy Section, Department of Biomedical Sciences and
Biotechnology, University of Brescia, Brescia, Italy; and the Institute
of Cytomorphology NP CNR, Bologna, Italy.
The pattern of expression of several protein kinase C (PKC) isoforms
( ,  I,  ,  ,  , and  ) during the course of
hematopoietic development was investigated using primary human
CD34+ hematopoietic cells and stable cell lines subcloned
from the growth factor-dependent 32D murine hematopoietic cell line.
Each 32D cell clone shows the phenotype and growth factor dependence characteristics of the corresponding hematopoietic lineage. Clear-cut differences were noticed between erythroid and nonerythroid lineages. (1) The functional inhibition of PKC- in primary human
CD34+ hematopoietic cells resulted in a twofold increase
in the number of erythroid colonies. (2) Erythroid 32D Epo1 cells
showed a lower level of bulk PKC catalytic activity, lacked the
expression of and PKC isoforms, and showed a weak or absent
upregulation of the remaining isoforms, except I, upon readdition
of Epo to growth factor-starved cells. (3) 32D, 32D GM1, and 32D G1
cell lines with mast cell, granulo-macrophagic, and granulocytic
phenotype, respectively, expressed all the PKC isoforms investigated,
but showed distinct responses to growth factor readdition. (4) 32D Epo
1.1, a clone selected for interleukin-3 (IL-3) responsiveness from 32D
Epo1, expressed the isoform only when cultured with IL-3. On the
other hand, when cultured in Epo, 32D Epo1.1 cells lacked the
expression of both and PKC isoforms, similarly to 32D Epo1. (5)
All 32D cell lines expressed the mRNA for PKC-, indicating that the
downmodulation of the isoform occurred at a posttranscriptional
level. In conclusion, the PKC isoform expression during hematopoiesis
appears to be lineage-specific and, at least partially, related to the
growth factor response.
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