|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 5 (March 1), 1999:
pp. 1482-1486
RAPID COMMUNICATION
Detection of Kaposi's Sarcoma Herpesvirus DNA Sequences in Multiple
Myeloma Bone Marrow Stromal Cells
Dharminder Chauhan,
Ajit Bharti,
Noopur Raje,
Eric Gustafson,
Geraldine S. Pinkus,
Jack L. Pinkus,
Gerrard Teoh,
Teru Hideshima,
Steve P. Treon,
Joyce D. Fingeroth, and
Kenneth C. Anderson
From the Department of Adult Oncology, Dana-Farber Cancer Institute
and the Department of Medicine, Harvard Medical School; the Department
of Pathology, Brigham and Womens Hospital, and the Department of
Pathology, Harvard Medical School, Boston, MA.
Whether Kaposi's sarcoma herpesvirus (KSHV) is associated with
multiple myeloma (MM) remains controversial. We assayed for KSHV DNA
sequences in long-term bone marrow stromal cells (BMSCs) from 26 patients with MM and 4 normal donors. Polymerase chain reaction (PCR)
using primers which amplify a KSHV gene sequence to yield a 233-bp
fragment (KS330233 within open reading frame 26) was
negative in all cases. Aliquots of these PCR products were used as
templates in subsequent nested PCR, with primers that amplify a 186-bp
product internal to KS330233. BMSCs from 24 of 26 (92%)
patients with MM and 1 of 4 normal donors were KSHV PCR+.
DNA sequence analyses showed interpatient specific mutations (2 to 3 bp). Both Southern blot and sequence analyses confirmed the specificity
of PCR results. The presence of the KSHV gene sequences was further
confirmed by amplifying T 1.1 (open reading frame [ORF]
K7) and viral cyclin D (ORF 72), two other domains within the KSHV
genome. Immunohistochemical studies of KSHV PCR+ MM BMSCs
demonstrate expression of dendritic cell (DC) lineage markers (CD68,
CD83, and fascin). Serological studies for the presence of KSHV lytic
or latent antibodies were performed using sera from 53 MM patients, 12 normal donors, and 5 human immunodeficiency virus
(HIV)/KSHV+ patients. No lytic or latent antibodies were
present in sera from either MM patients or normal donors. Taken
together, these findings show that KSHV DNA sequences are detectable in
BMSCs from the majority of MM patients, but that serologic responses to
KSHV are not present. Ongoing studies are defining whether the lack of
antibody response is caused by the absence of ongoing infection, the
presence of a novel viral strain associated with MM, or underlying
immunodeficiency in these patients.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Kukreja, S. Radfar, B.-H. Sun, K. Insogna, and M. V. Dhodapkar
Dominant role of CD47-thrombospondin-1 interactions in myeloma-induced fusion of human dendritic cells: implications for bone disease
Blood,
October 15, 2009;
114(16):
3413 - 3421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Dhodapkar, S. Barbuto, P. Matthews, A. Kukreja, A. Mazumder, D. Vesole, S. Jagannath, and M. V. Dhodapkar
Dendritic cells mediate the induction of polyfunctional human IL17-producing cells (Th17-1 cells) enriched in the bone marrow of patients with myeloma
Blood,
October 1, 2008;
112(7):
2878 - 2885.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Rappocciolo, F. J. Jenkins, H. R. Hensler, P. Piazza, M. Jais, L. Borowski, S. C. Watkins, and C. R. Rinaldo Jr
DC-SIGN Is a Receptor for Human Herpesvirus 8 on Dendritic Cells and Macrophages
J. Immunol.,
February 1, 2006;
176(3):
1741 - 1749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sergerie, Y. Abed, J. Roy, and G. Boivin
Comparative Evaluation of Three Serological Methods for Detection of Human Herpesvirus 8-Specific Antibodies in Canadian Allogeneic Stem Cell Transplant Recipients
J. Clin. Microbiol.,
June 1, 2004;
42(6):
2663 - 2667.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P Ryan, S Aarons, D Murray, T Markham, S O'Sullivan, F Lyons, G Lee, and J Fitzgibbon
Human herpesvirus 8 (HHV-8) detected in two patients with Kaposi's sarcoma-like pyogenic granuloma
J. Clin. Pathol.,
August 1, 2002;
55(8):
619 - 622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. V. Ablashi, L. G. Chatlynne, J. E. Whitman Jr., and E. Cesarman
Spectrum of Kaposi's Sarcoma-Associated Herpesvirus, or Human Herpesvirus 8, Diseases
Clin. Microbiol. Rev.,
July 1, 2002;
15(3):
439 - 464.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Brander, N. Raje, P. G. O'Connor, F. Davies, J. Davis, D. Chauhan, T. Hideshima, J. Martin, D. Osmond, D. H. Kedes, et al.
Absence of biologically important Kaposi sarcoma-associated herpesvirus gene products and virus-specific cellular immune responses in multiple myeloma
Blood,
June 28, 2002;
100(2):
698 - 700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Otieno, C. Banura, E. Katongole-Mbidde, J. L. Johnson, M. Ghannoum, A. Dowlati, R. Renne, E. Arts, C. Whalen, M. M. Lederman, et al.
Therapeutic Challenges of AIDS-Related Non-Hodgkin's Lymphoma in the United States and East Africa
J Natl Cancer Inst,
May 15, 2002;
94(10):
718 - 732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M.-Y. Sze, G. Giesajtis, R. D. Brown, M. Raitakari, J. Gibson, J. Ho, A. G. Baxter, B. Fazekas de St Groth, A. Basten, and D. E. Joshua
Clonal cytotoxic T cells are expanded in myeloma and reside in the CD8+CD57+CD28- compartment
Blood,
November 1, 2001;
98(9):
2817 - 2827.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Maeda, T. Niimi, S. Sato, H. Kawaguchi, Y. Sugiura, S. Mori, and R. Ueda
Human Herpesvirus 8 Is Not Associated With Sarcoidosis in Japanese Patients
Chest,
October 1, 2000;
118(4):
923 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. V. Ablashi, L. Chatlynne, D. Thomas, D. Bourboulia, M. B. Rettig, R. A. Vescio, D. Viza, P. Gill, R. A. Kyle, J. R. Berenson, et al.
Lack of serologic association of human herpesvirus-8 (KSHV) in patients with monoclonal gammopathy of undetermined significance with and without progression to multiple myeloma
Blood,
September 15, 2000;
96(6):
2304 - 2306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-J. Zhang, J.-H. Deng, C. Rabkin, and S.-J. Gao
Hot-spot variations of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen and application in genotyping by PCR-RFLP
J. Gen. Virol.,
August 1, 2000;
81(8):
2049 - 2058.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Hideshima, D. Chauhan, G. Teoh, N. Raje, S. P. Treon, Y.-T. Tai, Y. Shima, and K. C. Anderson
Characterization of Signaling Cascades Triggered by Human Interleukin-6 versus Kaposi's Sarcoma-associated Herpes Virus-encoded Viral Interleukin 6
Clin. Cancer Res.,
March 1, 2000;
6(3):
1180 - 1189.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Rebuttal to Tarte, Chang, and Klein
Blood,
May 15, 1999;
93(10):
3163 - 3164.
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Raje, J. Gong, D. Chauhan, G. Teoh, D. Avigan, Z. Wu, D. Chen, S. P. Treon, I. J. Webb, D. W. Kufe, et al.
Bone Marrow and Peripheral Blood Dendritic Cells From Patients With Multiple Myeloma Are Phenotypically and Functionally Normal Despite the Detection of Kaposi's Sarcoma Herpesvirus Gene Sequences
Blood,
March 1, 1999;
93(5):
1487 - 1495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
