|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 5 (March 1), 1999:
pp. 1715-1723
Heparin-Binding Epidermal Growth Factor-Like Growth
Factor/Diphtheria Toxin Receptor Expression by Acute Myeloid Leukemia
Cells
Fabrizio Vinante,
Antonella Rigo,
Emanuele Papini,
Marco A. Cassatella, and
Giovanni Pizzolo
From the Departments of Hematology and General Pathology, University
of Verona, Verona; and CNR Center for Biomembranes, University of
Padua, Padua, Italy.
Heparin-binding epidermal growth factor-like growth factor (HB-EGF)
is an EGF family member expressed by numerous cell types that binds to
EGF receptor 1 (HER-1) or 4 (HER-4) inducing mitogenic and/or
chemotactic activities. Membrane-bound HB-EGF retains growth activity
and adhesion capabilities and the unique property of being the receptor
for diphtheria toxin (DT). The interest in studying HB-EGF in acute
leukemia stems from these mitogenic, chemotactic, and receptor
functions. We analyzed the expression of HB-EGF in L428, Raji,
Jurkat, Karpas 299, L540, 2C8, HL-60, U937, THP-1, ML-3, and K562 cell
lines and in primary blasts from 12 acute myeloid leukemia (AML) cases,
by reverse-transcriptase polymerase chain reaction (RT-PCR) and
Northern blot and by the evaluation of sensitivity to DT. The release
of functional HB-EGF was assessed by evaluation of its proliferative
effects on the HB-EGF-sensitive Balb/c 3T3 cell line. HB-EGF was
expressed by all myeloid and T, but not B (L428, Raji), lymphoid cell
lines tested, as well as by the majority (8 of 12) of ex vivo AML
blasts. Cell lines (except for the K562 cell line) and AML blasts
expressing HB-EGF mRNA underwent apoptotic death following exposure to
DT, thus demonstrating the presence of the HB-EGF molecule on their membrane. Leukemic cells also released a fully functional HB-EGF molecule that was mitogenic for the Balb/c 3T3 cell line. Factors relevant to the biology of leukemic growth, such as tumor necrosis factor- (TNF-), 1,25-(OH)2D3, and
especially all-trans retinoic acid (ATRA), upregulated HB-EGF
mRNA in HL-60 or ML-3 cells. Granulocyte-macrophage colony-stimulating
factor (GM-CSF) induced HB-EGF mRNA and acquisition of sensitivity to
DT in one previously HB-EGF-negative leukemia case. Moreover, the U937
and Karpas 299 cell lines expressed HER-4 mRNA. This work shows that
HB-EGF is a growth factor produced by primary leukemic cells and
regulated by ATRA, 1,25-(OH)2D3, and GM-CSF.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Krampera, A. Pasini, A. Rigo, M. T. Scupoli, C. Tecchio, G. Malpeli, A. Scarpa, F. Dazzi, G. Pizzolo, and F. Vinante
HB-EGF/HER-1 signaling in bone marrow mesenchymal stem cells: inducing cell expansion and reversibly preventing multilineage differentiation
Blood,
July 1, 2005;
106(1):
59 - 66.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Vinante, A. Rigo, M. T. Scupoli, and G. Pizzolo
CD30 triggering by agonistic antibodies regulates CXCR4 expression and CXCL12 chemotactic activity in the cell line L540
Blood,
January 1, 2002;
99(1):
52 - 60.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Vinante, M. Marchi, A. Rigo, P. Scapini, G. Pizzolo, and M. A. Cassatella
Granulocyte-Macrophage Colony-Stimulating Factor Induces Expression of Heparin-Binding Epidermal Growth Factor-Like Growth Factor/Diphtheria Toxin Receptor and Sensitivity to Diphtheria Toxin in Human Neutrophils
Blood,
November 1, 1999;
94(9):
3169 - 3177.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
