|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 6 (March 15), 1999:
pp. 1969-1979
Inflammatory Cytokines and Vascular Endothelial Growth
Factor Stimulate the Release of Soluble Tie Receptor From
Human Endothelial Cells Via Metalloprotease Activation
Rachel Yabkowitz,
Susanne Meyer,
Tabitha Black,
Gary Elliott,
Lee
Anne Merewether, and
Harvey K. Yamane
From the Departments of Mammalian Cell Molecular Biology,
Experimental Hematology, Protein Structure, and Protein Chemistry,
Amgen Inc, Thousand Oaks, CA.
Activation of endothelial cells, important in processes such as
angiogenesis, is regulated by cell surface receptors, including those
in the tyrosine kinase (RTK) family. Receptor activity, in turn, can be
modulated by phosphorylation, turnover, or proteolytic release of a
soluble extracellular domain. Previously, we demonstrated that release
of soluble tie-1 receptor from endothelial cells by phorbol myristate
acetate (PMA) is mediated through protein kinase
C and a Ca2+-dependent protease. In this
study, the release of soluble tie-1 was shown to be stimulated by
inflammatory cytokines and vascular endothelial growth factor
(VEGF), but not by growth factors such as basic fibroblast
growth factor (bFGF) or transforming growth factor 
(TGF). Release of soluble tie by tumor necrosis factor (TNF) or VEGF occurred within 10 minutes of
stimulation and reached maximal levels within 60 minutes. Specificity
was shown by fluorescence-activated cell sorting (FACS) analysis;
endothelial cells exhibited a significant decrease in cell surface
tie-1 expression in response to TNF, whereas expression of epidermal
growth factor receptor (EGF-R) and CD31 was stable. In
contrast, tie-1 expression on megakaryoblastic UT-7 cells was
unaffected by PMA or TNF. Sequence analysis of the cleaved receptor
indicated that tie-1 was proteolyzed at the
E749/S750 peptide bond in the proximal
transmembrane domain. Moreover, the hydroxamic acid derivative BB-24
demonstrated dose-dependent inhibition of cytokine-, PMA-, and
VEGF-stimulated shedding, suggesting that the tie-1 protease was a
metalloprotease. Protease activity in a tie-1 peptide cleavage assay
was (1) associated with endothelial cell membranes, (2) specifically
activated in TNF-treated cells, and (3) inhibited by BB-24.
Additionally, proliferation of endothelial cells in response to VEGF,
but not bFGF, was inhibited by BB-24, suggesting that the release of
soluble tie-1 receptor plays a role in VEGF-mediated proliferation.
This study demonstrated that the release of soluble tie-1 from
endothelial cells is stimulated by inflammatory cytokines and VEGF
through the activation of an endothelial membrane-associated metalloprotease.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
F. Morello, A. Perino, and E. Hirsch
Phosphoinositide 3-kinase signalling in the vascular system
Cardiovasc Res,
May 1, 2009;
82(2):
261 - 271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Findley, R. G. Mitchell, B. D. Duscha, B. H. Annex, and C. D. Kontos
Plasma Levels of Soluble Tie2 and Vascular Endothelial Growth Factor Distinguish Critical Limb Ischemia From Intermittent Claudication in Patients With Peripheral Arterial Disease
J. Am. Coll. Cardiol.,
July 29, 2008;
52(5):
387 - 393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cai, O. Kehoe, G. M. Smith, P. Hykin, and M. E. Boulton
The Angiopoietin/Tie-2 System Regulates Pericyte Survival and Recruitment in Diabetic Retinopathy
Invest. Ophthalmol. Vis. Sci.,
May 1, 2008;
49(5):
2163 - 2171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Findley, M. J. Cudmore, A. Ahmed, and C. D. Kontos
VEGF Induces Tie2 Shedding via a Phosphoinositide 3-Kinase/Akt Dependent Pathway to Modulate Tie2 Signaling
Arterioscler. Thromb. Vasc. Biol.,
December 1, 2007;
27(12):
2619 - 2626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Marron, H. Singh, T. A. Tahir, J. Kavumkal, H.-Z. Kim, G. Y. Koh, and N. P. J. Brindle
Regulated Proteolytic Processing of Tie1 Modulates Ligand Responsiveness of the Receptor-tyrosine Kinase Tie2
J. Biol. Chem.,
October 19, 2007;
282(42):
30509 - 30517.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Lindsey, G. P. Escobar, L. W. Dobrucki, D. K. Goshorn, S. Bouges, J. T. Mingoia, D. M. McClister Jr., H. Su, J. Gannon, C. MacGillivray, et al.
Matrix metalloproteinase-9 gene deletion facilitates angiogenesis after myocardial infarction
Am J Physiol Heart Circ Physiol,
January 1, 2006;
290(1):
H232 - H239.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Gutwein, A. Stoeck, S. Riedle, D. Gast, S. Runz, T. P. Condon, A. Marme, M.-C. Phong, O. Linderkamp, A. Skorokhod, et al.
Cleavage of L1 in Exosomes and Apoptotic Membrane Vesicles Released from Ovarian Carcinoma Cells
Clin. Cancer Res.,
April 1, 2005;
11(7):
2492 - 2501.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Kunapuli, C. S. Kasyapa, L. Hawthorn, and J. K. Cowell
LGI1, a Putative Tumor Metastasis Suppressor Gene, Controls in Vitro Invasiveness and Expression of Matrix Metalloproteinases in Glioma Cells through the ERK1/2 Pathway
J. Biol. Chem.,
May 28, 2004;
279(22):
23151 - 23157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Stewart and B. L. Langille
Tied Down by Shear Force: Role for Tie1 in Postnatal Vascular Remodeling?
Circ. Res.,
February 20, 2004;
94(3):
271 - 272.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Raza, G. N. Fuller, C. H. Rhee, S. Huang, K. Hess, W. Zhang, and R. Sawaya
Identification of Necrosis-Associated Genes in Glioblastoma by cDNA Microarray Analysis
Clin. Cancer Res.,
January 1, 2004;
10(1):
212 - 221.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Karnani and K. Kairemo
The New Tie-1 Monoclonal Antibodies Detect Angiogenesis in Metastatic Malignancies
Clin. Cancer Res.,
September 1, 2003;
9(10):
3827S - 3830.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Takenobu, A. Yamazaki, M. Hirata, T. Umata, and E. Mekada
The Stress- and Inflammatory Cytokine-induced Ectodomain Shedding of Heparin-binding Epidermal Growth Factor-like Growth Factor Is Mediated by p38 MAPK, Distinct from the 12-O-Tetradecanoylphorbol-13-acetate- and Lysophosphatidic Acid-induced Signaling Cascades
J. Biol. Chem.,
May 2, 2003;
278(19):
17255 - 17262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. ILJIN, T. V. PETROVA, T. VEIKKOLA, V. KUMAR, M. POUTANEN, and K. ALITALO
A fluorescent Tie1 reporter allows monitoring of vascular development and endothelial cell isolation from transgenic mouse embryos
FASEB J,
November 1, 2002;
16(13):
1764 - 1774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. D. Kontos, E. H. Cha, J. D. York, and K. G. Peters
The Endothelial Receptor Tyrosine Kinase Tie1 Activates Phosphatidylinositol 3-Kinase and Akt To Inhibit Apoptosis
Mol. Cell. Biol.,
March 15, 2002;
22(6):
1704 - 1713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. ILAN, A. MOHSENIN, L. CHEUNG, and J. A. MADRI
PECAM-1 shedding during apoptosis generates a membrane-anchored truncated molecule with unique signaling characteristics
FASEB J,
February 1, 2001;
15(2):
362 - 372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D Nath, N. Williamson, R Jarvis, and G Murphy
Shedding of c-Met is regulated by crosstalk between a G-protein coupled receptor and the EGF receptor and is mediated by a TIMP-3 sensitive metalloproteinase
J. Cell Sci.,
January 3, 2001;
114(6):
1213 - 1220.
[Abstract]
[PDF]
|
|
|
|
|
|
|
D. MECHTCHERIAKOVA, G. SCHABBAUER, M. LUCERNA, M. CLAUSS, R. DE MARTIN, B. R. BINDER, and E. HOFER
Specificity, diversity, and convergence in VEGF and TNF-{alpha} signaling events leading to tissue factor up-regulation via EGR-1 in endothelial cells
FASEB J,
January 1, 2001;
15(1):
230 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. B. S. Henry and B. R. Duling
TNF-alpha increases entry of macromolecules into luminal endothelial cell glycocalyx
Am J Physiol Heart Circ Physiol,
December 1, 2000;
279(6):
H2815 - H2823.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Doedens and R. A. Black
Stimulation-induced Down-regulation of Tumor Necrosis Factor-alpha Converting Enzyme
J. Biol. Chem.,
May 5, 2000;
275(19):
14598 - 14607.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Marron, D. P. Hughes, M. D. Edge, C. L. Forder, and N. P. J. Brindle
Evidence for Heterotypic Interaction between the Receptor Tyrosine Kinases TIE-1 and TIE-2
J. Biol. Chem.,
December 8, 2000;
275(50):
39741 - 39746.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
