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Blood, Vol. 93 No. 9 (May 1), 1999:
pp. 2898-2906
Glycoprotein IIb-IIIa Is Expressed on Avian Multilineage Hematopoietic
Progenitor Cells
Christiane Ody,
Pierre Vaigot,
Pascale Quéré,
Beat A. Imhof, and
Catherine Corbel
From the Département de Pathologie, Centre Médical
Universitaire (CMU), Geneva, Switzerland; Institut d'Embryologie
Cellulaire et Moléculaire du Centre National de la Recherche
Scientifique (CNRS) et du Collège de France, Nogent/Marne,
France; and INRA, Station de Pathologie Aviaire, Nouzilly, France.
The fibrinogen receptor GPIIb-IIIa integrin is known to be expressed
on cells of the megakaryocytic lineage, but its presence on
hematopoietic progenitors has been a controversial issue. To resolve
this ambiguity unequivocally, we performed clonogenic assays and
intrathymic cell-transfer experiments in congenic animals. As the
ontogeny of the avian hematopoietic system is well documented, we used
this experimental model to trace GPIIb-IIIa expression during
embryogenesis. Consequently, we now report that the GPIIb-IIIa integrin
is expressed as early as embryonic day 3.5 (E3.5) to 4 in intraaortic
hematopoietic clusters, the first site of intraembryonic hematopoietic
progenitor emergence, and later in E6 paraaortic foci. Myeloid and
erythroid progenitors were also detected within the
GPIIb-IIIa+ CD45+ population isolated from
the E3.5 to 4 aortic area, while in embryonic and adult bone marrow,
myeloid, erythroid, and T-cell progenitors were present in the
GPIIb-IIIa+ c-kit+ population.
Furthermore, we also provide the first evidence, that
GPIIb-IIIa+ bone marrow cells can differentiate into T
cells. Hence, GPIIb-IIIa can be used as a marker for multilineage
hematopoietic progenitors, permitting identification of early
intraembryonic sites of hematopoiesis, as well as the isolation of
embryonic and adult hematopoietic progenitors.
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