|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 11 (December 1), 1999:
pp. 3722-3729
Differentiation Stage-Specific Regulation of Primitive Human
Hematopoietic Progenitor Cycling by Exogenous and Endogenous Inhibitors
in an In Vivo Model
J.D. Cashman,
I. Clark-Lewis,
A.C. Eaves, and
C.J. Eaves
From the Terry Fox Laboratory, British Columbia Cancer Agency,
Biomedical Research Centre; and Departments of Medical Genetics,
Biochemistry and Molecular Biology, Medicine, and Pathology and
Laboratory Medicine, University of British Columbia, Vancouver, British
Columbia, Canada.
Nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice
transplanted with human cord blood or adult marrow cells and injected 6 weeks posttransplant with 2 daily doses of transforming growth
factor- 1 (TGF-1), monocyte
chemoattractant protein-1 (MCP-1), or a nonaggregating form of
macrophage inflammatory protein-1 (MIP-1) showed unique patterns
of inhibition of human progenitor proliferation 1 day later.
TGF-1 was active on long-term culture initiating cells
(LTC-IC) and on primitive erythroid and granulopoietic colony-forming
cells (HPP-CFC), but had no effect on mature CFC. MCP-1 inhibited the
cycling of both types of HPP-CFC but not LTC-IC. MIP-1 did not
inhibit either LTC-IC or granulopoietic HPP-CFC but was active on
erythroid HPP-CFC and mature granulopoietic CFC. All of these responses
were independent of the source of human cells transplanted. LTC-IC of
either human cord blood or adult marrow origin continue to proliferate
in NOD/SCID mice for many weeks, although the turnover of all types of
human CFC in mice transplanted with adult human marrow (but not cord
blood) is downregulated after 6 weeks. Interestingly, administration of
either MIP-1, an antagonist of both MIP-1 and MCP-1 or
MCP-1(9-76), an antagonist of MCP-1 (and MCP-2 and MCP-3), into mice in
which human marrow-derived CFC had become quiescent, caused the rapid reactivation of these progenitors in vivo. These results provide the
first definition of stage-specific inhibitors of human hematopoietic progenitor cell cycling in vivo. In addition they show that endogenous chemokines can contribute to late graft failure, which can be reversed
by the administration of specific antagonists.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. Pelayo, K. Miyazaki, J. Huang, K. P. Garrett, D. G. Osmond, and P. W. Kincade
Cell Cycle Quiescence of Early Lymphoid Progenitors in Adult Bone Marrow
Stem Cells,
December 1, 2006;
24(12):
2703 - 2713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Yao, P. Li, Q. Zhang, E. M. Schwarz, P. Keng, A. Arbini, B. F. Boyce, and L. Xing
Tumor Necrosis Factor-{alpha} Increases Circulating Osteoclast Precursor Numbers by Promoting Their Proliferation and Differentiation in the Bone Marrow through Up-regulation of c-Fms Expression
J. Biol. Chem.,
April 28, 2006;
281(17):
11846 - 11855.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang, H. Hisha, S. Taketani, Y. Adachi, Q. Li, W. Cui, Y. Cui, J. Wang, C. Song, T. Mizokami, et al.
Characterization of Mesenchymal Stem Cells Isolated from Mouse Fetal Bone Marrow
Stem Cells,
March 1, 2006;
24(3):
482 - 493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang, H. Hisha, S. Taketani, M. Inaba, Q. Li, W. Cui, C. Song, T. Fan, Y. Cui, K. Guo, et al.
Neural Cell Adhesion Molecule Contributes to Hemopoiesis-Supporting Capacity of Stromal Cell Lines
Stem Cells,
September 1, 2005;
23(9):
1389 - 1399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Akel, C. Petrow-Sadowski, M. J. Laughlin, and F. W. Ruscetti
Neutralization of Autocrine Transforming Growth Factor-{beta} in Human Cord Blood CD34+CD38-Lin- Cells Promotes Stem-Cell-Factor-Mediated Erythropoietin-Independent Early Erythroid Progenitor Development and Reduces Terminal Differentiation
Stem Cells,
September 1, 2003;
21(5):
557 - 567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Liu, Y. Sun, F. Jiang, S. Zhang, Y. Wu, Y. Lan, X. Yang, and N. Mao
Disruption of Smad5 gene leads to enhanced proliferation of high-proliferative potential precursors during embryonic hematopoiesis
Blood,
January 1, 2003;
101(1):
124 - 133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cashman, B. Dykstra, I. Clark-Lewis, A. Eaves, and C. Eaves
Changes in the Proliferative Activity of Human Hematopoietic Stem Cells in NOD/SCID Mice and Enhancement of Their Transplantability after In Vivo Treatment with Cell Cycle Inhibitors
J. Exp. Med.,
November 4, 2002;
196(9):
1141 - 1150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm, P. Tang, I. Clark-Lewis, C. von Kalle, and C. Eaves
Ex vivo treatment of proliferating human cord blood stem cells with stroma-derived factor-1 enhances their ability to engraft NOD/SCID mice
Blood,
May 1, 2002;
99(9):
3454 - 3457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cashman, I. Clark-Lewis, A. Eaves, and C. Eaves
Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice
Blood,
February 1, 2002;
99(3):
792 - 799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Fan, G. Valdimarsdottir, J. Larsson, A. Brun, M. Magnusson, S. E. Jacobsen, P. ten Dijke, and S. Karlsson
Transient Disruption of Autocrine TGF-{beta} Signaling Leads to Enhanced Survival and Proliferation Potential in Single Primitive Human Hemopoietic Progenitor Cells
J. Immunol.,
January 15, 2002;
168(2):
755 - 762.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Cheng, H. Shen, N. Rodrigues, S. Stier, and D. T. Scadden
Transforming growth factor beta 1 mediates cell-cycle arrest of primitive hematopoietic cells independent of p21Cip1/Waf1 or p27Kip1
Blood,
December 15, 2001;
98(13):
3643 - 3649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Geiger, J. M. True, G. de Haan, and G. Van Zant
Age- and stage-specific regulation patterns in the hematopoietic stem cell hierarchy
Blood,
November 15, 2001;
98(10):
2966 - 2972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. D. Rossi, K. L. Medina, K. Garrett, G. Kolar, P. C. Comp, L. D. Shultz, J. D. Capra, P. Wilson, A. Schipul, and P. W. Kincade
Relatively Normal Human Lymphopoiesis but Rapid Turnover of Newly Formed B Cells in Transplanted Nonobese Diabetic/SCID Mice
J. Immunol.,
September 15, 2001;
167(6):
3033 - 3042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. Holyoake, X. Jiang, H. G. Jorgensen, S. Graham, M. J. Alcorn, C. Laird, A. C. Eaves, and C. J. Eaves
Primitive quiescent leukemic cells from patients with chronic myeloid leukemia spontaneously initiate factor-independent growth in vitro in association with up-regulation of expression of interleukin-3
Blood,
February 1, 2001;
97(3):
720 - 728.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Sugiura, H. Hisha, J. Ishikawa, Y. Adachi, S. Taketani, S. Lee, T. Nagahama, and S. Ikehara
Major Histocompatibility Complex Restriction Between Hematopoietic Stem Cells and Stromal Cells In Vitro
Stem Cells,
January 1, 2001;
19(1):
46 - 58.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Glimm, I.-H. Oh, and C. J. Eaves
Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G2/M transit and do not reenter G0
Blood,
December 15, 2000;
96(13):
4185 - 4193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. O. Fortunel, A. Hatzfeld, and J. A. Hatzfeld
Transforming growth factor-beta : pleiotropic role in the regulation of hematopoiesis
Blood,
September 15, 2000;
96(6):
2022 - 2036.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
