SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nakamura, Y. Articles by Kato, S. Search for Related Content PubMed PubMed Citation Articles by Nakamura, Y. Articles by Kato, S. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 94 No. 12 (December 15), 1999: pp. 4053-4059 Ex Vivo Generation of CD34+ Cells From CD34 Hematopoietic Cells Yoshihiko Nakamura, Kiyoshi Ando, Jamel Chargui, Hiroshi Kawada, Tadayuki Sato, Takashi Tsuji, Tomomitsu Hotta, and Shunichi Kato From the Research Center for Cell Transplantation, Department of Pediatrics, and the Department of Hematology, Tokai University, School of Medicine, Kanagawa, Japan; JT Inc, Kanagawa, Japan; and the Unit of Transplantation and Clinical Immunology, Claude Bernard University, Pavillon P, Hopital E. Herriot, Lyon, France. The human LinCD34 cell population contains a newly defined class of hematopoietic stem cells that reconstitute hematopoiesis in xenogeneic transplantation systems. We therefore developed a culture condition in which these cells were maintained and then acquired CD34 expression and the ability to produce colony-forming cells (CFC) and SCID-repopulating cells (SRCs). A murine bone marrow stromal cell line, HESS-5, supports the survival and proliferation of LinCD34 cells in the presence of fetal calf serum and human cytokines thrombopoietin, Flk-2/Flt-3 ligand, stem cell factor, granulocyte colony-stimulating factor, interleukin-3, and interleukin-6. Although LinCD34 cells do not initially form any hematopoietic colonies in methylcellulose, they do acquire the colony-forming ability during 7 days of culture, which coincides with their conversion to a CD34+ phenotype. From 2.2% to 12.1% of the cells became positive for CD34 after culture. The long-term multilineage repopulating ability of these cultured cells was also confirmed by transplantation into irradiated NOD/SCID mice. These results represent the first in vitro demonstration of the precursor of CD34+ cells in the human CD34 cell population. Furthermore, the in vitro system we reported here is expected to open the way to the precise characterization and ex vivo manipulation of LinCD34 hematopoietic stem cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. A. Hess, L. Wirthlin, T. P. Craft, P. E. Herrbrich, S. A. Hohm, R. Lahey, W. C. Eades, M. H. Creer, and J. A. Nolta Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells Blood, March 1, 2006; 107(5): 2162 - 2169. [Abstract] [Full Text] [PDF] E. B. Friedrich, K. Walenta, J. Scharlau, G. Nickenig, and N. Werner CD34-/CD133+/VEGFR-2+ Endothelial Progenitor Cell Subpopulation With Potent Vasoregenerative Capacities Circ. Res., February 17, 2006; 98(3): e20 - e25. [Abstract] [Full Text] [PDF] D. C. Dooley, B. K. Oppenlander, and M. Xiao Analysis of Primitive CD34- and CD34+ Hematopoietic Cells from Adults: Gain and Loss of CD34 Antigen by Undifferentiated Cells Are Closely Linked to Proliferative Status in Culture Stem Cells, July 1, 2004; 22(4): 556 - 569. [Abstract] [Full Text] [PDF] S. Konakahara, K. Ohashi, K. Mizuno, K. Itoh, and T. Tsuji CD29 integrin- and LIMK1/cofilin-mediated actin reorganization regulates the migration of haematopoietic progenitor cells underneath bone marrow stromal cells Genes Cells, April 1, 2004; 9(4): 345 - 358. [Abstract] [Full Text] [PDF] P. A. Dreyfus, F. Chretien, B. Chazaud, Y. Kirova, P. Caramelle, L. Garcia, G. Butler-Browne, and R. K. Gherardi Adult Bone Marrow-Derived Stem Cells in Muscle Connective Tissue and Satellite Cell Niches Am. J. Pathol., March 1, 2004; 164(3): 773 - 779. [Abstract] [Full Text] [PDF] P. Denning-Kendall, S. Singha, B. Bradley, and J. Hows Cobblestone Area-Forming Cells in Human Cord Blood Are Heterogeneous and Differ from Long-Term Culture-Initiating Cells Stem Cells, November 1, 2003; 21(6): 694 - 701. [Abstract] [Full Text] [PDF] S. Rutella, G. Bonanno, M. Marone, D. de Ritis, A. Mariotti, M. T. Voso, G. Scambia, S. Mancuso, G. Leone, and L. Pierelli Identification of a Novel Subpopulation of Human Cord Blood CD34-CD133-CD7-CD45+Lineage- Cells Capable of Lymphoid/NK Cell Differentiation After In Vitro Exposure to IL-15 J. Immunol., September 15, 2003; 171(6): 2977 - 2988. [Abstract] [Full Text] [PDF] Y. Torrente, G. Camirand, F. Pisati, M. Belicchi, B. Rossi, F. Colombo, M. El Fahime, N. J. Caron, A. C. Issekutz, G. Constantin, et al. Identification of a putative pathway for the muscle homing of stem cells in a muscular dystrophy model J. Cell Biol., August 4, 2003; 162(3): 511 - 520. [Abstract] [Full Text] [PDF] I. Dybedal, L. Yang, D. Bryder, I. Aastrand-Grundstrom, K. Leandersson, and S. E. W. Jacobsen Human reconstituting hematopoietic stem cells up-regulate Fas expression upon active cell cycling but remain resistant to Fas-induced suppression Blood, July 1, 2003; 102(1): 118 - 126. [Abstract] [Full Text] [PDF] T. Yahata, K. Ando, T. Sato, H. Miyatake, Y. Nakamura, Y. Muguruma, S. Kato, and T. Hotta A highly sensitive strategy for SCID-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow Blood, April 15, 2003; 101(8): 2905 - 2913. [Abstract] [Full Text] [PDF] J. Wang, T. Kimura, R. Asada, S. Harada, S. Yokota, Y. Kawamoto, Y. Fujimura, T. Tsuji, S. Ikehara, and Y. Sonoda SCID-repopulating cell activity of human cord blood-derived CD34- cells assured by intra-bone marrow injection Blood, April 15, 2003; 101(8): 2924 - 2931. [Abstract] [Full Text] [PDF] S. Kuci, J. T. Wessels, H.-J. Buhring, K. Schilbach, M. Schumm, G. Seitz, J. Loffler, P. Bader, P. G. Schlegel, D. Niethammer, et al. Identification of a novel class of human adherent CD34- stem cells that give rise to SCID-repopulating cells Blood, February 1, 2003; 101(3): 869 - 876. [Abstract] [Full Text] [PDF] Y. Guo, M. Lubbert, and M. Engelhardt CD34- Hematopoietic Stem Cells: Current Concepts and Controversies Stem Cells, January 1, 2003; 21(1): 15 - 20. [Abstract] [Full Text] [PDF] M. A. Dao, J. Arevalo, and J. A. Nolta Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution Blood, January 1, 2003; 101(1): 112 - 118. [Abstract] [Full Text] [PDF] J. Cashman, B. Dykstra, I. Clark-Lewis, A. Eaves, and C. Eaves Changes in the Proliferative Activity of Human Hematopoietic Stem Cells in NOD/SCID Mice and Enhancement of Their Transplantability after In Vivo Treatment with Cell Cycle Inhibitors J. Exp. Med., November 4, 2002; 196(9): 1141 - 1150. [Abstract] [Full Text] [PDF] O. Kollet, I. Petit, J. Kahn, S. Samira, A. Dar, A. Peled, V. Deutsch, M. Gunetti, W. Piacibello, A. Nagler, et al. Human CD34+CXCR4- sorted cells harbor intracellular CXCR4, which can be functionally expressed and provide NOD/SCID repopulation Blood, September 26, 2002; 100(8): 2778 - 2786. [Abstract] [Full Text] [PDF] G. H. Danet, J. L. Luongo, G. Butler, M. M. Lu, A. J. Tenner, M. C. Simon, and D. A. Bonnet C1qRp defines a new human stem cell population with hematopoietic and hepatic potential PNAS, August 6, 2002; 99(16): 10441 - 10445. [Abstract] [Full Text] [PDF] K. D. Bunting ABC Transporters as Phenotypic Markers and Functional Regulators of Stem Cells Stem Cells, January 1, 2002; 20(1): 11 - 20. [Abstract] [Full Text] [PDF] D. S. Kaufman, E. T. Hanson, R. L. Lewis, R. Auerbach, and J. A. Thomson Hematopoietic colony-forming cells derived from human embryonic stem cells PNAS, September 4, 2001; (2001) 191362598. [Abstract] [Full Text] [PDF] M. Harraz, C. Jiao, H. D. Hanlon, R. S. Hartley, and G. C. Schatteman CD34- Blood-Derived Human Endothelial Cell Progenitors Stem Cells, July 1, 2001; 19(4): 304 - 312. [Abstract] [Full Text] [PDF] Y. Yu, M. Hagihara, K. Ando, B. Gansuvd, H. Matsuzawa, T. Tsuchiya, Y. Ueda, H. Inoue, T. Hotta, and S. Kato Enhancement of Human Cord Blood CD34+ Cell-Derived NK Cell Cytotoxicity by Dendritic Cells J. Immunol., February 1, 2001; 166(3): 1590 - 1600. [Abstract] [Full Text] [PDF] Y. Torrente, J.-P Tremblay, F. Pisati, M. Belicchi, B. Rossi, M. Sironi, F. Fortunato, M. El Fahime, M. G. D'Angelo, N. J. Caron, et al. Intraarterial Injection of Muscle-derived CD34+Sca-1+ Stem Cells Restores Dystrophin in mdx Mice J. Cell Biol., January 22, 2001; 152(2): 335 - 348. [Abstract] [Full Text] [PDF] J. H. S. Kabarowski and O. N. Witte Consequences of BCR-ABL Expression within the Hematopoietic Stem Cell in Chronic Myeloid Leukemia Stem Cells, November 1, 2000; 18(6): 399 - 408. [Abstract] [Full Text] R. W. Storms, M. A. Goodell, A. Fisher, R. C. Mulligan, and C. Smith Hoechst dye efflux reveals a novel CD7+CD34- lymphoid progenitor in human umbilical cord blood Blood, September 15, 2000; 96(6): 2125 - 2133. [Abstract] [Full Text] [PDF] D. S. Kaufman, E. T. Hanson, R. L. Lewis, R. Auerbach, and J. A. Thomson Hematopoietic colony-forming cells derived from human embryonic stem cells PNAS, September 11, 2001; 98(19): 10716 - 10721. [Abstract] [Full Text] [PDF]

	Copyright © 1999 by American Society of Hematology         Online ISSN: 1528-0020