|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 12 (December 15), 1999:
pp. 4255-4262
c-myb Transactivates the Human Cyclin A1 Promoter and Induces Cyclin
A1 Gene Expression
Carsten Müller,
Rong Yang,
Gregory Idos,
Nicola Tidow,
Sven Diederichs,
Olaf M. Koch,
Walter Verbeek,
Timothy P. Bender, and
H.
Phillip Koeffler
From the Division of Hematology/Oncology, Cedars-Sinai Research
Institute/UCLA School of Medicine, Los Angeles, CA; the Department of
Microbiology, University of Virginia, Charlottesville, VA; and the
Department of Hematology/Oncology, University of Muenster, Muenster,
Germany.
Cyclin A1 differs from other cyclins in its highly restricted
expression pattern. Besides its expression during spermatogenesis, cyclin A1 is also expressed in hematopoietic progenitor cells and in
acute myeloid leukemia. We investigated mechanisms that might
contribute to cyclin A1 expression in hematopoietic cells. Comparison
of cyclin A1 and cyclin A promoter activity in adherent and myeloid
leukemia cell lines showed that the cyclin A1 promoter is
preferentially active in myeloid cell lines. This preferential activity
was present in a small, 335-bp cyclin A1 promoter fragment that
contained several potential c-myb binding sites. Coexpression of a
c-myb expression vector with the cyclin A1 promoter constructs significantly increased the reporter activity in adherent CV-1 as well
as in myeloid U937 cells. Gel-shift assays demonstrated that c-myb
could bind to the cyclin A1 promoter at a binding site located near the
transcription start site. Site-directed mutagenesis of this site
decreased promoter transactivation by 50% in both KCL22 cells that
express high levels of c-myb and in CV-1 cells that were transfected
with c-myb. In addition, transfection of primary human embryonic
fibroblasts with a c-myb expression vector led to induction of the
endogenous cyclin A1 gene. Taken together, c-myb can directly
transactivate the promoter of cyclin A1, and c-myb might be involved in
the high-level expression of cyclin A1 observed in acute myeloid
leukemia. These findings suggest that c-myb induces
hematopoiesis-specific mechanisms of cell cycle regulation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Restle, M. Farber, C. Baumann, M. Bohringer, K. H. Scheidtmann, C. Muller-Tidow, and L. Wiesmuller
Dissecting the role of p53 phosphorylation in homologous recombination provides new clues for gain-of-function mutants
Nucleic Acids Res.,
September 1, 2008;
36(16):
5362 - 5375.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-W. Jang, S.-j. Yang, A. Ehlen, S. Dong, H. Khoury, J. Chen, J. L. Persson, and K. Ye
Serine/Arginine Protein-Specific Kinase 2 Promotes Leukemia Cell Proliferation by Phosphorylating Acinus and Regulating Cyclin A1
Cancer Res.,
June 15, 2008;
68(12):
4559 - 4570.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Drabsch, H. Hugo, R. Zhang, D. H. Dowhan, Y. R. Miao, A. M. Gewirtz, S. C. Barry, R. G. Ramsay, and T. J. Gonda
Mechanism of and requirement for estrogen-regulated MYB expression in estrogen-receptor-positive breast cancer cells
PNAS,
August 21, 2007;
104(34):
13762 - 13767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Afroze, A. M. Sadi, M. A. Momen, S. Gu, S. Heximer, and M. Husain
c-Myb-Dependent Inositol 1,4,5-Trisphosphate Receptor Type-1 Expression in Vascular Smooth Muscle Cells
Arterioscler Thromb Vasc Biol,
June 1, 2007;
27(6):
1305 - 1311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Katabami, H. Donninger, F. Hommura, V. D. Leaner, I. Kinoshita, J. F. B. Chick, and M. J. Birrer
Cyclin A Is a c-Jun Target Gene and Is Necessary for c-Jun-induced Anchorage-independent Growth in RAT1a Cells
J. Biol. Chem.,
April 29, 2005;
280(17):
16728 - 16738.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Muller-Tidow, P. Ji, S. Diederichs, J. Potratz, N. Baumer, G. Kohler, T. Cauvet, C. Choudary, T. van der Meer, W.-Y. I. Chan, et al.
The Cyclin A1-CDK2 Complex Regulates DNA Double-Strand Break Repair
Mol. Cell. Biol.,
October 15, 2004;
24(20):
8917 - 8928.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Lele and D. J. Wolgemuth
Distinct Regions of the Mouse Cyclin A1 Gene, Ccna1, Confer Male Germ-Cell Specific Expression and Enhancer Function
Biol Reprod,
October 1, 2004;
71(4):
1340 - 1347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Araki, M. Ito, T. Soyano, R. Nishihama, and Y. Machida
Mitotic Cyclins Stimulate the Activity of c-Myb-like Factors for Transactivation of G2/M Phase-specific Genes in Tobacco
J. Biol. Chem.,
July 30, 2004;
279(31):
32979 - 32988.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Steffen, H. Serve, W. E. Berdel, S. Agrawal, B. Linggi, T. Buchner, S. W. Hiebert, and C. Muller-Tidow
Specific protein redirection as a transcriptional therapy approach for t(8;21) leukemia
PNAS,
July 8, 2003;
100(14):
8448 - 8453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cammenga, J. C. Mulloy, F. J. Berguido, D. MacGrogan, A. Viale, and S. D. Nimer
Induction of C/EBPalpha activity alters gene expression and differentiation of human CD34+ cells
Blood,
March 15, 2003;
101(6):
2206 - 2214.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Roudier, E. Fedorova, M. Lebris, P. Lecomte, J. Gyorgyey, D. Vaubert, G. Horvath, P. Abad, A. Kondorosi, and E. Kondorosi
The Medicago Species A2-Type Cyclin Is Auxin Regulated and Involved in Meristem Formation But Dispensable for Endoreduplication-Associated Developmental Programs
Plant Physiology,
March 1, 2003;
131(3):
1091 - 1103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Luger, S. G. O'Brien, J. Ratajczak, M. Z. Ratajczak, R. Mick, E. A. Stadtmauer, P. C. Nowell, J. M. Goldman, and A. M. Gewirtz
Oligodeoxynucleotide-mediated inhibition of c-myb gene expression in autografted bone marrow: a pilot study
Blood,
February 15, 2002;
99(4):
1150 - 1158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ito, S. Araki, S. Matsunaga, T. Itoh, R. Nishihama, Y. Machida, J. H. Doonan, and A. Watanabe
G2/M-Phase-Specific Transcription during the Plant Cell Cycle Is Mediated by c-Myb-Like Transcription Factors
PLANT CELL,
August 1, 2001;
13(8):
1891 - 1905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Muller-Tidow, W. Wang, G. E. Idos, S. Diederichs, R. Yang, C. Readhead, W. E. Berdel, H. Serve, M. Saville, R. Watson, et al.
Cyclin A1 directly interacts with B-myb and cyclin A1/cdk2 phosphorylate B-myb at functionally important serine and threonine residues: tissue-specific regulation of B-myb function
Blood,
April 1, 2001;
97(7):
2091 - 2097.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Muller, R. Yang, D. J. Park, H. Serve, W. E. Berdel, and H. P. Koeffler
The aberrant fusion proteins PML-RARalpha and PLZF-RARalpha contribute to the overexpression of cyclin A1 in acute promyelocytic leukemia
Blood,
December 1, 2000;
96(12):
3894 - 3899.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Müller, C. Readhead, S. Diederichs, G. Idos, R. Yang, N. Tidow, H. Serve, W. E. Berdel, and H. P. Koeffler
Methylation of the Cyclin A1 Promoter Correlates with Gene Silencing in Somatic Cell Lines, while Tissue-Specific Expression of Cyclin A1 Is Methylation Independent
Mol. Cell. Biol.,
May 1, 2000;
20(9):
3316 - 3329.
[Abstract]
[Full Text]
|
|
|
|
|
