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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Payne, K. J. Articles by Kincade, P. W. Search for Related Content PubMed PubMed Citation Articles by Payne, K. J. Articles by Kincade, P. W. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 94 No. 2 (July 15), 1999: pp. 713-723 Loss of c-kit Accompanies B-Lineage Commitment and Acquisition of CD45R by Most Murine B-Lymphocyte Precursors Kimberly J. Payne, Kay L. Medina, and Paul W. Kincade From the Oklahoma Medical Research Foundation, Immunobiology and Cancer Program, Oklahoma City, OK. Using surface markers, we identified two bone marrow (BM) subsets enriched for TdT+ cells on the brink of CD45R acquisition. These two populations, Linc-kitLo and Linc-kit, consisting of 35.4% and 7.4%, respectively, TdT+ cells, generated B-lineage cells in overnight cultures. Approximately half of the c-kitLo B-lineage precursors were bipotential, yielding myeloid and lymphoid progeny, whereas most that were c-kit gave rise only to lymphocytes. Analysis of B-lineage progression during a finite culture period showed that the most mature precursors were concentrated in the Linc-kit population. Moreover, a majority of the earliest CD45R+ pro-B cells in BM, identified as CD45R+ CD43+ BP-1 CD25 natural killer (NK)1.1 sIgM, were also c-kit. These c-kit cells, like their c-kitLo counterparts, expressed TdT, proliferated in response to interleukin (IL)-7, and generated sIgM+ cells. These data suggest that TdT expression is initiated as c-kit downregulation begins in Lin cells, with progressive loss of c-kit during B-lineage differentiation. CD45R expression is initiated during the transition from c-kitLo to c-kit with many cells losing c-kit before acquiring CD45R. The ability to isolate highly enriched populations of viable CD45R precursors will be instrumental in characterizing the earliest B-lineage cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. A. Dudakov, G. L. Goldberg, J. J. Reiseger, A. P. Chidgey, and R. L. Boyd Withdrawal of Sex Steroids Reverses Age- and Chemotherapy-Related Defects in Bone Marrow Lymphopoiesis J. Immunol., May 15, 2009; 182(10): 6247 - 6260. [Abstract] [Full Text] [PDF] G. E. Desanti, A. Cumano, and R. Golub Identification of CD4int progenitors in mouse fetal spleen, a source of resident lymphoid cells J. Leukoc. 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