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Next Article 
Blood, Vol. 94 No. 3 (August 1), 1999:
pp. 837-844
Serial Analysis of Gene Expression in Human Monocytes and Macrophages
Shin-ichi Hashimoto,
Takuji Suzuki,
Hong-Yan Dong,
Nobuyuki Yamazaki, and
Kouji Matsushima
From the Department of Molecular Preventive Medicine and CREST,
School of Medicine, The University of Tokyo, Tokyo, Japan.
Monocytes/macrophages serve as sentinels involved in chronic
inflammation and the eradication of various pathogens. To define molecularly the differentiation of blood monocytes into macrophages, we
conducted serial analysis of gene expression (SAGE) in human blood
monocytes/macrophages induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) or M-CSF. SAGE analysis of 57,560, 57,463, and 55,856 tags from monocytes, GM-CSF-, and M-CSF-induced macrophages, respectively, allowed identification of 35,037 different transcripts. Interestingly, the genes with the highest expression during differentiation from monocytes into macrophages were genes involved in lipid metabolism. Both CSF-induced macrophages expressed similar sets of genes except for several genes such as monocyte-derived chemokine (MDC), legumain, prostaglandin D synthetase, and lysosomal sialoglycoprotein. The identification of specific gene expression in
human monocytes, GM-CSF-, or M-CSF-induced macrophages provides novel
methods to define macrophage subsets and the maturation and activation
stage of cells of macrophage lineage and, possibly, to diagnose
diseases in which macrophages play a major role. This study represents
the first extensive serial analysis of gene expression for any type of
human hematopoietic cells.
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