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Next Article 
Blood, Vol. 94 No. 5 (September 1), 1999:
pp. 1495-1503
Functional and Molecular Analysis of Hematopoietic Progenitors Derived
From the Aorta-Gonad-Mesonephros Region of the Mouse Embryo
Sylvie Delassus,
Ian Titley, and
Tariq Enver
From the Section of Gene Function and Regulation and the Leukaemia
Research Fund Centre, Institute of Cancer Research, Chester Beatty
Laboratories, London, UK.
Herein, we show that CD34, c-kit double-positive
(CD34+c-kit+) cells from the
aorta-gonad-mesonephros (AGM) region of the developing mouse are
multipotent in vitro and can undergo both B-lymphoid and multimyeloid
differentiation. Molecular analysis of individual CD34+c-kit+ cells by single-cell reverse
transcriptase-polymerase chain reaction (RT-PCR) shows coactivation of
erythroid ( -globin) and myeloid (myeloperoxidase [MPO]) but not
lymphoid-affiliated (CD3, Thy-1, and  5) genes. Additionally, most
cells coexpress the stem cell-associated transcriptional regulators
AML-1, PU.1, GATA-2 and Lmo2, as well as the granulocyte
colony-stimulating factor receptor (G-CSF-R). These results show that
the CD34+c-kit+ population from the AGM
represents a highly enriched source of multipotent hematopoietic cells,
and suggest that limited coactivation of distinct lineage-affiliated
genes is an early event in the generation of hematopoietic stem and
progenitor cells during ontogeny.
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