|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 7 (October 1), 1999:
pp. 2301-2309
Neuropilin-1 Is Expressed on Bone Marrow Stromal Cells: A Novel
Interaction With Hematopoietic Cells?
Rafaèle Tordjman,
Nathalie Ortéga,
Laure Coulombel,
Jean Plouët,
Paul-Henri Roméo, and
Valérie Lemarchandel
From INSERM U474, Hopital Henri Mondor, Créteil, France;
Laboratoire de Biologie Moléculaire Eucaryote, CNRS UPR 9006, Toulouse, France; and INSERM U363, IGR, Villejuif, France.
In adult bone marrow, hematopoietic stem cells are found in close
association with distinctive stromal cell elements. This association is
necessary for maintenance of hematopoiesis, but the precise mechanisms
underlying the cross-talk between stromal cells and hematopoietic stem
cells are poorly understood. In this study, we used a bone marrow
stromal cell line (MS-5) that is able to support human long-term
hematopoiesis. This hematopoietic-promoting activity cannot be related
to expression of known cytokines and is abolished by addition of
hydrocortisone. Using a gene trap strategy that selects genes encoding
transmembrane or secreted proteins expressed by MS-5 cells, we obtained
several insertions that produced fusion proteins. In one clone, fusion
protein activity was downregulated in the presence of hydrocortisone,
and we show that insertion of the trap vector has occurred into the
neuropilin-1 gene. Neuropilin-1 is expressed in MS-5 cells, in other
hematopoietic-supporting cell lines, and in primary stromal cells but
not in primitive hematopoietic cells. We show that neuropilin-1 acts as
a functional cell-surface receptor in MS-5 cells. Two neuropilin-1
ligands, semaphorin III and VEGF 165, can bind to these cells, and the addition of VEGF 165 to MS-5 cells increases expression of 2 cytokines known to regulate early hematopoiesis, Tpo and Flt3-L. Finally, we show
that stromal cells and immature hematopoietic cells express different
neuropilin-1 ligands. We propose that neuropilin-1 may act as a novel
receptor on stromal cells by mediating interactions between stroma and
primitive hematopoietic cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Z. Belaid-Choucair, Y. Lepelletier, G. Poncin, A. Thiry, C. Humblet, M. Maachi, A. Beaulieu, E. Schneider, A. Briquet, P. Mineur, et al.
Human Bone Marrow Adipocytes Block Granulopoiesis Through Neuropilin-1-Induced Granulocyte Colony-Stimulating Factor Inhibition
Stem Cells,
June 1, 2008;
26(6):
1556 - 1564.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nakayama, N. Mutsuga, and G. Tosato
FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc
Blood,
February 15, 2007;
109(4):
1363 - 1372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Narazaki and G. Tosato
Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A
Blood,
May 15, 2006;
107(10):
3892 - 3901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kirito, N. Fox, N. Komatsu, and K. Kaushansky
Thrombopoietin enhances expression of vascular endothelial growth factor (VEGF) in primitive hematopoietic cells through induction of HIF-1{alpha}
Blood,
June 1, 2005;
105(11):
4258 - 4263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Parikh, F. Fan, W. B. Liu, S. A. Ahmad, O. Stoeltzing, N. Reinmuth, D. Bielenberg, C. D. Bucana, M. Klagsbrun, and L. M. Ellis
Neuropilin-1 in Human Colon Cancer: Expression, Regulation, and Role in Induction of Angiogenesis
Am. J. Pathol.,
June 1, 2004;
164(6):
2139 - 2151.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. H. Underhill, D. George, E. G. Bremer, and G. S. Kansas
Gene expression profiling reveals a highly specialized genetic program of plasma cells
Blood,
May 15, 2003;
101(10):
4013 - 4021.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yamada, Y. Oike, H. Ogawa, Y. Ito, H. Fujisawa, T. Suda, and N. Takakura
Neuropilin-1 on hematopoietic cells as a source of vascular development
Blood,
March 1, 2003;
101(5):
1801 - 1809.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Matthies, Q. E. H. Low, M. W. Lingen, and L. A. DiPietro
Neuropilin-1 Participates in Wound Angiogenesis
Am. J. Pathol.,
January 1, 2002;
160(1):
289 - 296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Li and T. G. Rossman
Genes Upregulated in Lead-Resistant Glioma Cells Reveal Possible Targets for Lead-Induced Developmental Neurotoxicity
Toxicol. Sci.,
November 1, 2001;
64(1):
90 - 99.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. S. Steeg
Collapsin Response Mediator Protein-1: a Lung Cancer Invasion Suppressor Gene With Nerve
J Natl Cancer Inst,
September 19, 2001;
93(18):
1364 - 1365.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Delaire, C. Billard, R. Tordjman, A. Chedotal, A. Elhabazi, A. Bensussan, and L. Boumsell
Biological Activity of Soluble CD100. II. Soluble CD100, Similarly to H-SemaIII, Inhibits Immune Cell Migration
J. Immunol.,
April 1, 2001;
166(7):
4348 - 4354.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tordjman, S. Delaire, J. Plouet, S. Ting, P. Gaulard, S. Fichelson, P.-H. Romeo, and V. Lemarchandel
Erythroblasts are a source of angiogenic factors
Blood,
April 1, 2001;
97(7):
1968 - 1974.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yamada, N. Takakura, H. Yasue, H. Ogawa, H. Fujisawa, and T. Suda
Exogenous clustered neuropilin 1 enhances vasculogenesis and angiogenesis
Blood,
March 15, 2001;
97(6):
1671 - 1678.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. T. Bellamy, L. Richter, D. Sirjani, C. Roxas, B. Glinsmann-Gibson, Y. Frutiger, T. M. Grogan, and A. F. List
Vascular endothelial cell growth factor is an autocrine promoter of abnormal localized immature myeloid precursors and leukemia progenitor formation in myelodysplastic syndromes
Blood,
March 1, 2001;
97(5):
1427 - 1434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Pavelock, K. M. Braas, L'H. Ouafik, G. Osol, and V. May
Differential Expression and Regulation of the Vascular Endothelial Growth Factor Receptors Neuropilin-1 and Neuropilin-2 in Rat Uterus
Endocrinology,
February 1, 2001;
142(2):
613 - 622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Robinson and S. Stringer
The splice variants of vascular endothelial growth factor (VEGF) and their receptors
J. Cell Sci.,
January 3, 2001;
114(5):
853 - 865.
[Abstract]
[PDF]
|
|
|
|
|
|
|
H.-Q. MIAO, P. LEE, H. LIN, S. SOKER, and M. KLAGSBRUN
Neuropilin-1 expression by tumor cells promotes tumor angiogenesis and progression
FASEB J,
December 1, 2000;
14(15):
2532 - 2539.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. B. Whitaker, B. J. Limberg, and J. S. Rosenbaum
Vascular Endothelial Growth Factor Receptor-2 and Neuropilin-1 Form a Receptor Complex That Is Responsible for the Differential Signaling Potency of VEGF165 and VEGF121
J. Biol. Chem.,
June 29, 2001;
276(27):
25520 - 25531.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
