|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 7 (October 1), 1999:
pp. 2461-2468
Calreticulin and Calreticulin Fragments Are Endothelial Cell
Inhibitors That Suppress Tumor Growth
Sandra E. Pike,
Lei Yao,
Joyce Setsuda,
Karen D. Jones,
Barry Cherney,
Ettore Appella,
Kazuyasu Sakaguchi,
Hira Nakhasi,
Chintamani
D. Atreya,
Julie Teruya-Feldstein,
Peter Wirth,
Ghanshyam Gupta, and
Giovanna Tosato
From the Center for Biologics Evaluation and Research,
Rockville; the Laboratory of Cell Biology,
National Cancer Institute, DBS, Bethesda;
the Laboratory of Pathology, National Cancer
Institure, DCS, Bethesda; and the Laboratory of
Experimental Carcinogenesis, National Cancer Institute, DBS,
Bethesda, MD.
Several angiogenesis inhibitors are fragments of larger proteins
that are themselves not active as angiogenesis inhibitors. Vasostatin,
the N-terminal domain of calreticulin inclusive of amino acids 1-180, is an angiogenesis inhibitor that exerts antitumor effects in vivo. In
the present study, we examined whether the full-length calreticulin
molecule shares the antiangiogenic and antitumor activities of
vasostatin. Similar to vasostatin, calreticulin selectively inhibited
endothelial cell proliferation in vitro, but not cells of other
lineages, and suppressed angiogenesis in vivo. When inoculated into
athymic mice, calreticulin inhibited Burkitt tumor growth comparably
with vasostatin. Calreticulin lacking the N-terminal 1-120 amino acids
inhibited endothelial cell proliferation in vitro and Burkitt tumor
growth in vivo comparably with vasostatin. An internal calreticulin
fragment encompassing amino acids 120-180 also inhibited endothelial
cell proliferation in vitro and angiogenesis in vivo comparably with
calreticulin and vasostatin. These results suggest that the
antiangiogenic activities of vasostatin reside in a domain that is
accessible from the full-length calreticulin molecule and localize to
calreticulin N-terminal amino acids 120-180. Thus, calreticulin and
calreticulin fragments are inhibitors of angiogenesis that directly
target endothelial cells, inhibit angiogenesis, and suppress tumor
growth. This information may be critical in designing targeted
inhibitors of pathological angiogenesis that underlies cancer and other diseases.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Hassanein, A. S. Bojja, L. Glazewski, G. Lu, and R.W. Mason
Protein processing by the placental protease, cathepsin P
Mol. Hum. Reprod.,
July 1, 2009;
15(7):
433 - 442.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. D. Karagiannis and A. S. Popel
A systematic methodology for proteome-wide identification of peptides inhibiting the proliferation and migration of endothelial cells
PNAS,
September 16, 2008;
105(37):
13775 - 13780.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V.Y. Gu, M.H. Wong, J.L. Stevenson, K.E. Crawford, S.P. Brennecke, and N.M. Gude
Calreticulin in human pregnancy and pre-eclampsia
Mol. Hum. Reprod.,
May 1, 2008;
14(5):
309 - 315.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. X. Cai, L. Y. Tse, C. Leung, P. K.H. Tam, R. Xu, and M. H. Sham
Suppression of Lung Tumor Growth and Metastasis in Mice by Adeno-Associated Virus-Mediated Expression of Vasostatin
Clin. Cancer Res.,
February 1, 2008;
14(3):
939 - 949.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Osborne, S. Welle, S. L. Venance, C. A. Thornton, and R. Tawil
Expression profile of FSHD supports a link between retinal vasculopathy and muscular dystrophy
Neurology,
February 20, 2007;
68(8):
569 - 577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Pal, J. Wu, J. K. Murray, S. H. Gellman, M. A. Wozniak, P. J. Keely, M. E. Boyer, T. M. Gomez, S. M. Hasso, J. F. Fallon, et al.
An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis
J. Cell Biol.,
September 25, 2006;
174(7):
1047 - 1058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L Li, Y-Z Yuan, J Lu, L Xia, Y Zhu, Y-P Zhang, and M-M Qiao
Treatment of pancreatic carcinoma by adenoviral mediated gene transfer of vasostatin in mice
Gut,
February 1, 2006;
55(2):
259 - 265.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nakayama, N. Mutsuga, L. Yao, and G. Tosato
Prostaglandin E2 promotes degranulation-independent release of MCP-1 from mast cells
J. Leukoc. Biol.,
January 1, 2006;
79(1):
95 - 104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. K. Hong, P. Kumar, L. Wang, B. Damania, M. L. Gulley, H.-J. Delecluse, P. J. Polverini, and S. C. Kenney
Epstein-Barr Virus Lytic Infection Is Required for Efficient Production of the Angiogenesis Factor Vascular Endothelial Growth Factor in Lymphoblastoid Cell Lines
J. Virol.,
November 15, 2005;
79(22):
13984 - 13992.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Nyberg, L. Xie, and R. Kalluri
Endogenous Inhibitors of Angiogenesis
Cancer Res.,
May 15, 2005;
65(10):
3967 - 3979.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. M. Hsu, F. J. Hsieh, Y. M. Jeng, M. L. Kuo, C. N. Chen, D. M. Lai, L. J. Hsieh, B. T. Wang, P. N. Tsao, H. Lee, et al.
Calreticulin expression in neuroblastoma--a novel independent prognostic factor
Ann. Onc.,
February 1, 2005;
16(2):
314 - 321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Shen, M. D. Person, J. Zhu, J. L. Abbruzzese, and D. Li
Protein Expression Profiles in Pancreatic Adenocarcinoma Compared with Normal Pancreatic Tissue and Tissue Affected by Pancreatitis as Detected by Two-Dimensional Gel Electrophoresis and Mass Spectrometry
Cancer Res.,
December 15, 2004;
64(24):
9018 - 9026.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Berwin, Y. Delneste, R. V. Lovingood, S. R. Post, and S. V. Pizzo
SREC-I, a Type F Scavenger Receptor, Is an Endocytic Receptor for Calreticulin
J. Biol. Chem.,
December 3, 2004;
279(49):
51250 - 51257.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Vucenik, A. Passaniti, M. I. Vitolo, K. Tantivejkul, P. Eggleton, and A. M. Shamsuddin
Anti-angiogenic activity of inositol hexaphosphate (IP6)
Carcinogenesis,
November 1, 2004;
25(11):
2115 - 2123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. B. G. Gebbink, E. E. Voest, and A. Reijerkerk
Do antiangiogenic protein fragments have amyloid properties?
Blood,
September 15, 2004;
104(6):
1601 - 1605.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. H. Sage, M. Reed, S. E. Funk, T. Truong, M. Steadele, P. Puolakkainen, D. H. Maurice, and J. A. Bassuk
Cleavage of the Matricellular Protein SPARC by Matrix Metalloproteinase 3 Produces Polypeptides That Influence Angiogenesis
J. Biol. Chem.,
September 26, 2003;
278(39):
37849 - 37857.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Goicoechea, M. A. Pallero, P. Eggleton, M. Michalak, and J. E. Murphy-Ullrich
The Anti-adhesive Activity of Thrombospondin Is Mediated by the N-terminal Domain of Cell Surface Calreticulin
J. Biol. Chem.,
September 27, 2002;
277(40):
37219 - 37228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Albrecht and M. D. Lairmore
Critical Role of Human T-Lymphotropic Virus Type 1 Accessory Proteins in Viral Replication and Pathogenesis
Microbiol. Mol. Biol. Rev.,
September 1, 2002;
66(3):
396 - 406.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Bernardi, C. S. Johnson, R. A. Modzelewski, and D. L. Trump
Antiproliferative Effects of 1{alpha},25-Dihydroxyvitamin D3 and Vitamin D Analogs on Tumor-Derived Endothelial Cells
Endocrinology,
July 1, 2002;
143(7):
2508 - 2514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Yao, S. E. Pike, and G. Tosato
Laminin binding to the calreticulin fragment vasostatin regulates endothelial cell function
J. Leukoc. Biol.,
January 1, 2002;
71(1):
47 - 53.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. PONCE, M. NOMIZU, and H. K. KLEINMAN
An angiogenic laminin site and its antagonist bind through the {alpha}v{beta}3 and {alpha}5{beta}1 integrins
FASEB J,
June 1, 2001;
15(8):
1389 - 1397.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Amann and E. Ritz
Microvascular disease--the Cinderella of uraemic heart disease
Nephrol. Dial. Transplant.,
October 1, 2000;
15(10):
1493 - 1503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Yao, S. E. Pike, J. Setsuda, J. Parekh, G. Gupta, M. Raffeld, E. S. Jaffe, and G. Tosato
Effective targeting of tumor vasculature by the angiogenesis inhibitors vasostatin and interleukin-12
Blood,
September 1, 2000;
96(5):
1900 - 1905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Goicoechea, A. W. Orr, M. A. Pallero, P. Eggleton, and J. E. Murphy-Ullrich
Thrombospondin Mediates Focal Adhesion Disassembly through Interactions with Cell Surface Calreticulin
J. Biol. Chem.,
November 10, 2000;
275(46):
36358 - 36368.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Le Naour, L. Hohenkirk, A. Grolleau, D. E. Misek, P. Lescure, J. D. Geiger, S. Hanash, and L. Beretta
Profiling Changes in Gene Expression during Differentiation and Maturation of Monocyte-derived Dendritic Cells Using Both Oligonucleotide Microarrays and Proteomics
J. Biol. Chem.,
May 18, 2001;
276(21):
17920 - 17931.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
