|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 8 (October 15), 1999:
pp. 2595-2604
Symmetry of Initial Cell Divisions Among Primitive Hematopoietic
Progenitors Is Independent of Ontogenic Age and Regulatory
Molecules
Shiang Huang,
Ping Law,
Karl Francis,
Bernhard O. Palsson, and
Anthony D. Ho
From the Departments of Medicine and Bioengineering, University of
California, San Diego, CA; and the Department of Medicine V, University
of Heidelberg, Heidelberg, Germany.
We have developed a time-lapse camera system to follow the
replication history and the fate of hematopoietic stem cells (HSC) at a
single-cell level. Combined with single-cell culture, we correlated the
early replication behavior with colony development after 14 days. The
membrane dye PKH26 was used to monitor cell division. In addition to
multiple, synchronous, and symmetric divisions, single-sorted
CD34+/CD38 cells derived from fetal liver
(FLV) also gave rise to a daughter cell that remained quiescent for up
to 8 days, whereas the other daughter cell proliferated exponentially.
Upon separation and replating as single cells onto medium containing a
cytokine cocktail, 60.6% ± 9.8% of the initially quiescent cells
(PKH26 bright) gave rise again to colonies and 15.8% ± 7.8% to
blast colonies that could be replated. We have then determined the
effects of various regulatory molecules on symmetry of initial cell
divisions. After single-cell sorting, the
CD34+/CD38 cells derived from FLV were
exposed to flt3-ligand, thrombopoietin, stem cell factor (SCF), or
medium containing a cytokine cocktail (with SCF, interleukin-3,
interleukin-6, granulocyte-macrophage colony-stimulating factor, and
erythropoietin). Whereas mitotic rate, colony efficiency, and
asymmetric divisions could be altered using various regulatory
molecules, the asymmetric division index, defined as the number of
asymmetric divisions versus the number of dividing cells, was not
altered significantly. This observation suggests that, although lineage
commitment and cell proliferation can be skewed by extrinsic signaling,
symmetry of early divisions is probably under the control of intrinsic factors.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Fukushima, I. Matsumura, S. Ezoe, M. Tokunaga, M. Yasumi, Y. Satoh, H. Shibayama, H. Tanaka, A. Iwama, and Y. Kanakura
FIP1L1-PDGFR{alpha} Imposes Eosinophil Lineage Commitment on Hematopoietic Stem/Progenitor Cells
J. Biol. Chem.,
March 20, 2009;
284(12):
7719 - 7732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Beckmann, S. Scheitza, P. Wernet, J. C. Fischer, and B. Giebel
Asymmetric cell division within the human hematopoietic stem and progenitor cell compartment: identification of asymmetrically segregating proteins
Blood,
June 15, 2007;
109(12):
5494 - 5501.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gottschling, R. Saffrich, A. Seckinger, U. Krause, K. Horsch, K. Miesala, and A. D. Ho
Human Mesenchymal Stromal Cells Regulate Initial Self-Renewing Divisions of Hematopoietic Progenitor Cells by a {beta}1-Integrin-Dependent Mechanism
Stem Cells,
March 1, 2007;
25(3):
798 - 806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Giebel, T. Zhang, J. Beckmann, J. Spanholtz, P. Wernet, A. D. Ho, and M. Punzel
Primitive human hematopoietic cells give rise to differentially specified daughter cells upon their initial cell division
Blood,
March 1, 2006;
107(5):
2146 - 2152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wagner, S. Laufs, J. Blake, C. Schwager, X. Wu, J. W. Zeller, A. D. Ho, and S. Fruehauf
Retroviral Integration Sites Correlate with Expressed Genes in Hematopoietic Stem Cells
Stem Cells,
September 1, 2005;
23(8):
1050 - 1058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wagner, R. Saffrich, U. Wirkner, V. Eckstein, J. Blake, A. Ansorge, C. Schwager, F. Wein, K. Miesala, W. Ansorge, et al.
Hematopoietic Progenitor Cells and Cellular Microenvironment: Behavioral and Molecular Changes upon Interaction
Stem Cells,
September 1, 2005;
23(8):
1180 - 1191.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Rambhatla, S. Ram-Mohan, J. J. Cheng, and J. L. Sherley
Immortal DNA Strand Cosegregation Requires p53/IMPDH-Dependent Asymmetric Self-renewal Associated with Adult Stem Cells
Cancer Res.,
April 15, 2005;
65(8):
3155 - 3161.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. F. Srour, X. Tong, K. W. Sung, P. A. Plett, S. Rice, J. Daggy, C. T. Yiannoutsos, R. Abonour, and C. M. Orschell
Modulation of in vitro proliferation kinetics and primitive hematopoietic potential of individual human CD34+CD38-/lo cells in G0
Blood,
April 15, 2005;
105(8):
3109 - 3116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-M. Paulus, N. Debili, F. Larbret, J. Levin, and W. Vainchenker
Thrombopoietin responsiveness reflects the number of doublings undergone by megakaryocyte progenitors
Blood,
October 15, 2004;
104(8):
2291 - 2298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wagner, A. Ansorge, U. Wirkner, V. Eckstein, C. Schwager, J. Blake, K. Miesala, J. Selig, R. Saffrich, W. Ansorge, et al.
Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis
Blood,
August 1, 2004;
104(3):
675 - 686.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Ho and M. Punzel
Hematopoietic stem cells: can old cells learn new tricks?
J. Leukoc. Biol.,
May 1, 2003;
73(5):
547 - 555.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Merok, J. A. Lansita, J. R. Tunstead, and J. L. Sherley
Cosegregation of Chromosomes Containing Immortal DNA Strands in Cells That Cycle with Asymmetric Stem Cell Kinetics
Cancer Res.,
December 1, 2002;
62(23):
6791 - 6795.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Sherley
Asymmetric Cell Kinetics Genes: The Key to Expansion of Adult Stem Cells in Culture
Stem Cells,
November 1, 2002;
20(6):
561 - 572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Mantel, P. Hendrie, and H. E. Broxmeyer
Steel Factor Regulates Cell Cycle Asymmetry
Stem Cells,
November 1, 2001;
19(6):
483 - 491.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Tremain, J. Korkko, D. Ibberson, G. C. Kopen, C. DiGirolamo, and D. G. Phinney
MicroSAGE Analysis of 2,353 Expressed Genes in a Single Cell-Derived Colony of Undifferentiated Human Mesenchymal Stem Cells Reveals mRNAs of Multiple Cell Lineages
Stem Cells,
September 1, 2001;
19(5):
408 - 418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Sharma, M. C. Nelson, J. E. Brandt, M. Wessman, N. Mahmud, K. P. Weller, and R. Hoffman
Human CD34+ stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi
Blood,
January 15, 2001;
97(2):
426 - 434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm, I.-H. Oh, and C. J. Eaves
Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G2/M transit and do not reenter G0
Blood,
December 15, 2000;
96(13):
4185 - 4193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Domen and I. L. Weissman
Hematopoietic Stem Cells Need Two Signals to Prevent Apoptosis; BCL-2 Can Provide One of These, Kitl/c-Kit Signaling the Other
J. Exp. Med.,
December 11, 2000;
192(12):
1707 - 1718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. S. Kabarowski and O. N. Witte
Consequences of BCR-ABL Expression within the Hematopoietic Stem Cell in Chronic Myeloid Leukemia
Stem Cells,
November 1, 2000;
18(6):
399 - 408.
[Abstract]
[Full Text]
|
|
|
|
|
