|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 9 (November 1), 1999:
pp. 3055-3061
Seeding Efficiency of Primitive Human Hematopoietic Cells in Nonobese
Diabetic/Severe Combined Immune Deficiency Mice: Implications for Stem
Cell Frequency Assessment
Paula B. van Hennik,
Alexandra E. de Koning, and
Rob E. Ploemacher
From the Institute of Hematology, Erasmus University Rotterdam,
Rotterdam, The Netherlands.
Nonobese diabetic/severe combined immune deficiency (NOD/SCID) mouse
repopulating cells (SRC) have been proposed to represent a more
primitive human stem cell subset than the cobblestone area-forming cell
(CAFC) week (wk) 6 or the long-term culture-initiating cell (LTC-IC) wk
5 on the basis of their difference in frequency, phenotype, transfectibility, and multilineage outgrowth potential in
immunodeficient recipients. We have assessed the
percentage of various progenitor cell populations (colony-forming cell
[CFC] and CAFC subsets) contained in unsorted NOD/SCID BM nucleated
cells (nc), human umbilical cord blood (UCB) nc, bone marrow (BM) nc,
peripheral blood stem cells (PBSC), and CD34+ selected
UCB nc, seeding in the BM and spleen of NOD/SCID mice within 24 hours
after transplantation. The seeding efficiency of NOD/SCID BM CAFC wk 5 was median (range) in the spleen 2.9% (0.7% to 4.0%) and in the
total BM 8.7% (2.0% to 9.2%). For human unsorted UCB nc, BM nc,
PBSC, and CD34+ UCB cells, the seeding efficiency for
CAFC wk 6 in the BM of NOD/SCID mice was 4.4% (3.5% to 6.3%), 0.8%
(0.3% to 1.7%), 5.3% (1.4% to 13.6%), and 4.4% (3.5% to 6.3%),
respectively. Using flow cytometry, the percentage CD34+
UCB cells retrieved from the BM of sublethally or supralethally irradiated NOD/SCID mice was 2.3 (1.4 to 2.8) and 2.5 (1.6 to 2.7),
respectively. Because we did not observe any significant differences in
the seeding efficiencies of the various stem cell subsets, it may be
assumed that the SRC seeding efficiency in NOD/SCID mice is similarly
low. Our data indicate that the seeding efficiency of a graft can be of
great influence when assessing stem cell frequencies in in vivo
repopulation assays.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
H. Lin, E. De Stanchina, X. K. Zhou, Y. She, D. Hoang, S. W. Cheung, B. Cassileth, and S. Cunningham-Rundles
Maitake Beta-Glucan Enhances Umbilical Cord Blood Stem Cell Transplantation in the NOD/SCID Mouse
Experimental Biology and Medicine,
March 1, 2009;
234(3):
342 - 353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Horn and R. Blasczyk
Severe Combined Immunodeficiency-Repopulating Cell Assay May Overestimate Long-Term Repopulation Ability
Stem Cells,
December 1, 2007;
25(12):
3271 - 3272.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Inaba, Y. Adachi, H. Hisha, N. Hosaka, M. Maki, Y. Ueda, Y. Koike, T. Miyake, J. Fukui, Y. Cui, et al.
Extensive Studies on Perfusion Method Plus Intra-Bone Marrow-Bone Marrow Transplantation Using Cynomolgus Monkeys
Stem Cells,
August 1, 2007;
25(8):
2098 - 2103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Li, H. Hisha, R. Yasumizu, T.-X. Fan, G.-X. Yang, Q. Li, Y.-Z. Cui, X.-L. Wang, C.-Y. Song, S. Okazaki, et al.
Analyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes
Stem Cells,
May 1, 2007;
25(5):
1186 - 1194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Horn, H.-P. Kiem, K. Ando, and T. Yahata
Expansion of SCID repopulating cells does not prove expansion of hematopoietic stem cells.
Blood,
July 15, 2006;
108(2):
771 - 772.
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Lapidot, A. Dar, and O. Kollet
How do stem cells find their way home?
Blood,
September 15, 2005;
106(6):
1901 - 1910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. E. Daldrup-Link, M. Rudelius, G. Piontek, S. Metz, R. Brauer, G. Debus, C. Corot, J. Schlegel, T. M. Link, C. Peschel, et al.
Migration of Iron Oxide-labeled Human Hematopoietic Progenitor Cells in a Mouse Model: In Vivo Monitoring with 1.5-T MR Imaging Equipment
Radiology,
January 1, 2005;
234(1):
197 - 205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Ahmed, S. J. Ings, A. R. Pizzey, M. P. Blundell, A. J. Thrasher, H. T. Ye, A. Fahey, D. C. Linch, and K. L. Yong
Impaired bone marrow homing of cytokine-activated CD34+ cells in the NOD/SCID model
Blood,
March 15, 2004;
103(6):
2079 - 2087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, G. Muramoto, J. Fung, and C. Oxford
Quantitative Analysis Demonstrates Expansion of SCID-Repopulating Cells and Increased Engraftment Capacity in Human Cord Blood Following Ex Vivo Culture with Human Brain Endothelial Cells
Stem Cells,
March 1, 2004;
22(2):
202 - 215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-C. Lee, T. Cheng, G. B. Adams, E. C. Attar, N. Miura, S. B. Lee, Y. Saito, I. Olszak, D. Dombkowski, D. P. Olson, et al.
P2Y-like receptor, GPR105 (P2Y14), identifies and mediates chemotaxis of bone-marrowhematopoietic stem cells
Genes & Dev.,
July 1, 2003;
17(13):
1592 - 1604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yahata, K. Ando, T. Sato, H. Miyatake, Y. Nakamura, Y. Muguruma, S. Kato, and T. Hotta
A highly sensitive strategy for SCID-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow
Blood,
April 15, 2003;
101(8):
2905 - 2913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, A. A. Saini, D. J. Chute, M. R. Wells, W. B. Clark, D. M. Harlan, J. Park, M. K. Stull, C. Civin, and T. A. Davis
Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow
Blood,
December 15, 2002;
100(13):
4433 - 4439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, M. Bhatia, H. H. H. Kanhai, R. Breese, D. K. Heilman, C. S. Johnson, J. H. F. Falkenburg, and E. F. Srour
Engraftment potential of human fetal hematopoietic cells in NOD/SCID mice is not restricted to mitotically quiescent cells
Blood,
June 17, 2002;
100(1):
120 - 127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Jetmore, P. A. Plett, X. Tong, F. M. Wolber, R. Breese, R. Abonour, C. M. Orschell-Traycoff, and E. F. Srour
Homing efficiency, cell cycle kinetics, and survival of quiescent and cycling human CD34+ cells transplanted into conditioned NOD/SCID recipients
Blood,
March 1, 2002;
99(5):
1585 - 1593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Papayannopoulou, G. V. Priestley, B. Nakamoto, V. Zafiropoulos, and L. M. Scott
Molecular pathways in bone marrow homing: dominant role of {alpha}4{beta}1 over {beta}2-integrins and selectins
Blood,
October 15, 2001;
98(8):
2403 - 2411.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. C. C. Kerre, G. De Smet, M. De Smedt, F. Offner, J. De Bosscher, J. Plum, and B. Vandekerckhove
Both CD34+38+ and CD34+38- Cells Home Specifically to the Bone Marrow of NOD/LtSZ scid/scid Mice but Show Different Kinetics in Expansion
J. Immunol.,
October 1, 2001;
167(7):
3692 - 3698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Prosper and C. M. Verfaillie
Regulation of hematopoiesis through adhesion receptors
J. Leukoc. Biol.,
March 1, 2001;
69(3):
307 - 316.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Rosu-Myles, L. Gallacher, B. Murdoch, D. A. Hess, M. Keeney, D. Kelvin, L. Dale, S. S. G. Ferguson, D. Wu, F. Fellows, et al.
The human hematopoietic stem cell compartment is heterogeneous for CXCR4 expression
PNAS,
December 19, 2000;
97(26):
14626 - 14631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Cashman and C. J. Eaves
High marrow seeding efficiency of human lymphomyeloid repopulating cells in irradiated NOD/SCID mice
Blood,
December 1, 2000;
96(12):
3979 - 3981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, J. H. F. Falkenburg, X. Tong, W. A. Noort, R. Breese, D. Heilman, H. Kanhai, C. M. Orschell-Traycoff, and E. F. Srour
Similar repopulating capacity of mitotically active and resting umbilical cord blood CD34+ cells in NOD/SCID mice
Blood,
September 15, 2000;
96(6):
2100 - 2107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
