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Blood, Vol. 94 No. 9 (November 1), 1999:
pp. 3151-3160
Overexpression of CCAAT Displacement Protein Represses the
Promiscuously Active Proximal gp91phox Promoter
Diana Catt,
Shannon Hawkins,
Ann Roman,
Wen Luo, and
David G. Skalnik
From the Departments of Pediatrics, Biochemistry & Molecular Biology,
and Microbiology & Immunology, Section of Pediatric
Hematology/Oncology, Herman B Wells Center for Pediatric Research,
Indiana University School of Medicine and Walther Cancer Institute,
Indianapolis, IN.
CCAAT displacement protein (CDP) is a transcriptional repressor that
restricts expression of the gp91phox gene to mature
myeloid cells. CDP interacts with multiple sites within the  450 to
+12 bp human gp91phox promoter, and
down-regulation of CDP DNA-binding activity is required for induction
of gp91phox transcription in mature phagocytes.
Truncation of the gp91phox promoter to 102 to
+12 bp removes 4 CDP-binding sites and reveals a promiscuous promoter
activity that is active in some nonphagocytic cells. A cis-element at
90 bp is required for derepressed transcription and serves as a
binding site for multiple transcriptional activators. We now report
that this element also serves as a binding site for CDP. The affinity
of CDP for this element is relatively weak compared with upstream
CDP-binding sites within the promoter, consistent with the promiscuous
transcriptional activity exhibited by the 102 to +12 bp
gp91phox promoter fragment. Further analysis of the
proximal promoter reveals an additional weak-affinity CDP-binding site
centered at approximately 20 bp. Overexpression of cloned CDP
represses the 102 to +12 bp gp91phox promoter,
indicating that these proximal CDP-binding sites are functionally
significant. The constellation of transcriptional activators and a
repressor that interacts with the 90 bp cis-element is identical to
that observed for a promoter element at 220 bp, reflecting the
highly modular organization of the gp91phox
promoter. These studies illustrate the complex interplay between transcriptional activators and a repressor that contribute to the
myeloid-restricted expression of the gp91phox gene.
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