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Blood, Vol. 95 No. 1 (January 1), 2000:
pp. 277-285
Differential expression of Rel/NF- B and octamer factors is a
hallmark of the generation and maturation of dendritic cells
M. Neumann,
H.-W. Fries,
C. Scheicher,
P. Keikavoussi,
A. Kolb-Mäurer,
E.-B. Bröcker,
E. Serfling, and
E. Kämpgen
From the Institute of Pathology, Department of Molecular Pathology,
and the University Hospital, Department of Dermatology, University of
Würzburg, 97080 Würzburg, Germany.
A key feature of maturation of dendritic cells is the
down-regulation of antigen-processing and up-regulation of
immunostimulatory capacities. To study the differential expression of
transcription factors in this process, we investigated the nuclear
translocation and DNA binding of Rel/NF- B and octamer factors
during in vitro generation and maturation of dendritic cells compared
with macrophage development. RelB was the only factor strongly
up-regulated during the generation of both immature dendritic cells and
macrophages. Cytokine-induced maturation of dendritic
cells resulted in an increase in nuclear RelB, p50, p52, and especially
c-Rel, whereas cytokine-treated macrophages responded poorly. This
up-regulation of NF-B factors did not correlate with lower
levels of cytosolic NF-B inhibitors, the IBs. One IB, Bcl-3,
was strongly expressed only in mature dendritic cells. Furthermore,
generation and maturation of dendritic cells led to a continuous
down-regulation of the octamer factor Oct-2, whereas monocytes and
macrophages displayed high Oct-2 levels. A similar pattern of
maturation-induced changes in transcription factor levels was found in
cultured murine epidermal Langerhans cells, suggesting a general
physiological significance of these findings. Finally, this pattern of
differential activation of Rel and octamer factors appears to be
suitable in determining the maturation stage of dendritic cells
generated by treatment with different cytokine combinations in vitro.
(Blood. 2000;95:277-285)
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