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 Previous Article | Table of Contents
Blood, Vol. 95 No. 1 (January 1), 2000:
pp. 360-362
BRIEF REPORT
Single-tube multiplex-PCR screen for common
deletional determinants of  -thalassemia
Samuel S. Chong,
Corinne D. Boehm,
Douglas R. Higgs, and
Garry R. Cutting
From the McKusick-Nathans Institute of Genetic Medicine and
Department of Pediatrics, Johns Hopkins School of Medicine, Baltimore,
Maryland; the Departments of Pediatrics and Obstetrics & Gynecology,
National University of Singapore, Singapore; and the MRC Molecular
Hematology Unit, Institute of Molecular Medicine, Oxford, England.
 -Thalassemia is very common throughout all tropical and
subtropical regions of the world. In Southeast Asia and the
Mediterranean regions, compound heterozygotes and homozygotes may have
anemia that is mild to severe (hemoglobin [Hb] H disease) or lethal
(Hb Bart's hydrops fetalis). We have developed a reliable, single-tube multiplex-polymerase chain reaction (PCR) assay for the 6 most frequently observed determinants of -thalassemia. The
assay allows simple, high throughput genetic screening for these
common hematological disorders. (Blood. 2000;95:360-362)
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