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Blood, Vol. 95 No. 10 (May 15), 2000:
pp. 3102-3105
 2 Microglobulin-deficient (B2mnull)
NOD/SCID mice are excellent recipients for studying human stem cell
function
Orit Kollet,
Amnon Peled,
Tamara Byk,
Herzl Ben-Hur,
Dale Greiner,
Leonard Shultz, and
Tsvee Lapidot
From the Department of Immunology, The Weizmann Institute and the
Department of Obstetrics and Gynecology, Kaplan Hospital, Rehovot,
Israel; the University of Massachusetts, Worcester MA; and The Jackson
Laboratory, Bar Harbor, ME.
Human SCID repopulating cells (SRC) are defined based on their
functional ability to repopulate the bone marrow of NOD/SCID mice with
both myeloid and lymphoid cell populations. The frequency of SRC in
umbilical cord blood cells is 1 in 9.3 × 105
mononuclear cells. We report that as few as
8 × 104 human cord blood mononuclear cells transplanted
into NOD/SCID/B2mnull mice resulted in mutlilineage
differentiation in the murine bone marrow, revealing a more than
11-fold higher SRC frequency than in NOD/SCID mice. Moreover, as few as
2 to 5 × 103 CD34+ cells recovered from
the bone marrow of primary transplanted NOD/SCID mice were sufficient
for engrafting secondary NOD/SCID/B2mnull mice with
SRC, suggesting SRC self-renewal. Thus, by using
NOD/SCID/B2mnull mice as recipients, we established
a functional assay for human stem cells capable of engrafting the bone
marrow of primary and secondary transplanted immune-deficient mice with
SRC, providing a model that better resembles autologous stem cell transplantation.
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