|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 95 No. 10 (May 15), 2000:
pp. 3146-3152
Interleukin-13 induces PSGL-1/P-selectin-dependent adhesion of
eosinophils, but not neutrophils, to human umbilical vein
endothelial cells under flow
Gerrit Woltmann,
Claire A. McNulty,
Grant Dewson,
Fiona A. Symon, and
Andrew J. Wardlaw
From the Division of Respiratory Medicine, University of Leicester
School of Medicine, Glenfield Hospital, Leicester, UK.
Selective eosinophil accumulation is a hallmark of diseases such as
asthma. In a model of chronic eosinophilic inflammation, we have
previously shown that the tethering step in eosinophil adhesion is
mediated by PSGL-1 binding to P-selectin. The Th2-associated cytokine
IL-13 is of potential importance in allergic disease. We have therefore
investigated whether IL-13 can mediate eosinophil binding to human
umbilical vein endothelial cells (HUVEC) through P-selectin. IL-13
caused dose- and time-dependent increases of P-selectin expression, as
assessed by flow and laser scanning cytometry. A similar degree of
expression was observed with IL-4. There was no effect on E-selectin or
ICAM-1 expression. Tumor necrosis factor- induced the
expression of VCAM-1, E-selectin, and ICAM-1 but had no effect on
P-selectin expression. IL-13 increased the production of mRNA for
surface and soluble variants of P-selectin. Under flow conditions,
eosinophils, but not neutrophils, showed enhanced binding to
IL-13 and to IL-4-stimulated HUVEC compared to
medium-cultured cells. Eosinophil adhesion was completely inhibited by
a blocking monoclonal antibody against PSGL-1 and P-selectin. Anti-VLA-4 and anti-VCAM-1 antibodies inhibited binding to a lesser extent. Thus, at physiologic levels of expression induced by Th2 cytokines, P-selectin/PSGL-1 supported eosinophil but not neutrophil adhesion. This mechanism is likely to be a key event leading to the
selective accumulation of eosinophils in allergic inflammation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. Petri, M. Phillipson, and P. Kubes
The Physiology of Leukocyte Recruitment: An In Vivo Perspective
J. Immunol.,
May 15, 2008;
180(10):
6439 - 6446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Siddiqui, F. Hollins, S. Saha, and C. E. Brightling
Inflammatory cell microlocalisation and airway dysfunction: cause and effect?
Eur. Respir. J.,
December 1, 2007;
30(6):
1043 - 1056.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Mateo, Y. Naim Abu Nabah, M. Losada, R. Estelles, C. Company, B. Bedrina, J. M. Cerda-Nicolas, S. Poole, P. J. Jose, J. Cortijo, et al.
A critical role for TNF{alpha} in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles
Blood,
September 15, 2007;
110(6):
1895 - 1902.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Miyazaki, T. Satoh, K. Nishioka, and H. Yokozeki
STAT-6-Mediated Control of P-Selectin by Substance P and Interleukin-4 in Human Dermal Endothelial Cells
Am. J. Pathol.,
August 1, 2006;
169(2):
697 - 707.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Sayasith, N. Bouchard, D. Boerboom, K. A. Brown, M. Dore, and J. Sirois
Molecular Characterization of Equine P-Selectin (CD62P) and Its Regulation in Ovarian Follicles During the Ovulatory Process
Biol Reprod,
March 1, 2005;
72(3):
736 - 744.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Alvarez, M. Cerda-Nicolas, Y. Naim Abu Nabah, M. Mata, A. C. Issekutz, J. Panes, R. R. Lobb, and M.-J. Sanz
Direct evidence of leukocyte adhesion in arterioles by angiotensin II
Blood,
July 15, 2004;
104(2):
402 - 408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. H. Ulfman, D. P. H. Joosten, C. W. van Aalst, J.-W. J. Lammers, E. A. van de Graaf, L. Koenderman, and J. J. Zwaginga
Platelets Promote Eosinophil Adhesion of Patients with Asthma to Endothelium under Flow Conditions
Am. J. Respir. Cell Mol. Biol.,
April 1, 2003;
28(4):
512 - 519.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Y. Larbi, J. P. Dangerfield, F. J. Culley, D. Marshall, D. O. Haskard, P. J. Jose, T. J. Williams, and S. Nourshargh
P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses
J. Leukoc. Biol.,
January 1, 2003;
73(1):
65 - 73.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M P Ainslie, C A McNulty, T Huynh, F A Symon, and A J Wardlaw
Characterisation of adhesion receptors mediating lymphocyte adhesion to bronchial endothelium provides evidence for a distinct lung homing pathway
Thorax,
December 1, 2002;
57(12):
1054 - 1059.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Woerly, P. Lacy, A. B. Younes, N. Roger, S. Loiseau, R. Moqbel, and M. Capron
Human eosinophils express and release IL-13 following CD28-dependent activation
J. Leukoc. Biol.,
October 1, 2002;
72(4):
769 - 779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. H. Broide, M. Miller, D. Castaneda, J. Nayar, J. Y. Cho, M. Roman, L. G. Ellies, and P. Sriramarao
Core 2 oligosaccharides mediate eosinophil and neutrophil peritoneal but not lung recruitment
Am J Physiol Lung Cell Mol Physiol,
February 1, 2002;
282(2):
L259 - L266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Cuvelier and K. D. Patel
Shear-dependent Eosinophil Transmigration on Interleukin 4-stimulated Endothelial Cells: A Role for Endothelium-associated Eotaxin-3
J. Exp. Med.,
December 10, 2001;
194(12):
1699 - 1709.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
