|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 95 No. 12 (June 15), 2000:
pp. 3990-3995
Longitudinal monitoring of immune reconstitution by CDR3 size
spectratyping after T-cell-depleted allogeneic bone marrow transplant
and the effect of donor lymphocyte infusions on T-cell repertoire
Stephanie Verfuerth,
Karl Peggs,
Paulomi Vyas,
Lorna Barnett,
Richard J. O'Reilly, and
Stephen Mackinnon
From the Department of Haematology, University College London,
London, United Kingdom; and Bone Marrow Transplant Service, MSKCC,
New York, NY.
Delayed immune reconstitution after allogeneic bone marrow
transplantation (BMT) with associated infection is a major cause of
morbidity and mortality. We used third complementarity region (CDR3)
size spectratyping as a tool for monitoring T-cell repertoire reconstitution in 19 patients over a median time of 40 months after
T-cell-depleted allogeneic BMT for chronic myeloid leukemia (CML).
Furthermore, the effect of donor lymphocyte infusions (DLI) for the
treatment of relapse in 18 of the 19 patients was analyzed. All BMT
recipients had irregular spectratypes in the first 3- to -6 months
after transplant. These evolved to more normal patterns by 12 months
after transplant and continued to improve thereafter. In approximately
a third of the patients, it took 2 to 3 years for all spectratypes to
normalize, whereas in the other two thirds, some abnormal spectratypes
persisted even after several years. In 9 patients, there was no
immediate change in the CDR3 size profiles after DLI. In 3 patients,
spectratypes improved slightly after DLI, whereas in 6 patients,
spectratypes became more restricted and irregular. Overall, T-cell
spectratypes in BMT patients were characterized by instability over
time and in patients with graft-versus-host disease (GVHD), this was
even more exaggerated. Several factors, such as pre-BMT conditioning,
T-cell depletion of the donor marrow, loss of thymic function in
adults, exposure to infectious agents, GVHD, and immunosuppressive
treatment, are likely contributors to the delay in T-cell-repertoire reconstitution.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
W. J. Murphy
Raising the spectra of T-cell profiling
Blood,
October 15, 2008;
112(8):
3008 - 3009.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Truong and D. B. McGavern
A Novel Virus Carrier State to Evaluate Immunotherapeutic Regimens: Regulatory T Cells Modulate the Pathogenicity of Antiviral Memory Cells
J. Immunol.,
July 15, 2008;
181(2):
1161 - 1169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Michalek, I. Kocak, V. Fait, J. Zaloudik, and R. Hajek
Detection and Long-Term In Vivo Monitoring of Individual Tumor-Specific T Cell Clones in Patients with Metastatic Melanoma
J. Immunol.,
June 1, 2007;
178(11):
6789 - 6795.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Meyer, C. M. Britten, D. Wehler, K. Bender, G. Hess, A. Konur, U. F. Hartwig, T. C. Wehler, A. J. Ullmann, C. Gentilini, et al.
Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell-depleted reduced-intensity transplantation
Blood,
January 1, 2007;
109(1):
374 - 382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Recchia, C. Bonini, Z. Magnani, F. Urbinati, D. Sartori, S. Muraro, E. Tagliafico, A. Bondanza, M. T. L. Stanghellini, M. Bernardi, et al.
Retroviral vector integration deregulates gene expression but has no consequence on the biology and function of transplanted T cells
PNAS,
January 31, 2006;
103(5):
1457 - 1462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Bozza, K. Perruccio, C. Montagnoli, R. Gaziano, S. Bellocchio, E. Burchielli, G. Nkwanyuo, L. Pitzurra, A. Velardi, and L. Romani
A dendritic cell vaccine against invasive aspergillosis in allogeneic hematopoietic transplantation
Blood,
November 15, 2003;
102(10):
3807 - 3814.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Michalek, R. H. Collins, H. P. Durrani, P. Vaclavkova, L. E. Ruff, D. C. Douek, and E. S. Vitetta
Definitive separation of graft-versus-leukemia- and graft-versus-host-specific CD4+ T cells by virtue of their receptor beta loci sequences
PNAS,
February 4, 2003;
100(3):
1180 - 1184.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Mufti, A. F. List, S. D. Gore, and A. Y.L. Ho
Myelodysplastic Syndrome
Hematology,
January 1, 2003;
2003(1):
176 - 199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Barrett, K. Rezvani, S. Solomon, A. M. Dickinson, X. N. Wang, G. Stark, H. Cullup, M. Jarvis, P. G. Middleton, and N. Chao
New Developments in Allotransplant Immunology
Hematology,
January 1, 2003;
2003(1):
350 - 371.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. N. Kochenderfer, S. Kobayashi, E. D. Wieder, C. Su, and J. J. Molldrem
Loss of T-lymphocyte clonal dominance in patients with myelodysplastic syndrome responsive to immunosuppression
Blood,
November 15, 2002;
100(10):
3639 - 3645.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hummel, D. Wilms, M. Vitacolonna, and M. Zoller
Donor T cell and host NK depletion improve the therapeutic efficacy of allogeneic bone marrow cell reconstitution in the nonmyeloablatively conditioned tumor-bearing host
J. Leukoc. Biol.,
November 1, 2002;
72(5):
898 - 912.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Risitano, H. Kook, W. Zeng, G. Chen, N. S. Young, and J. P. Maciejewski
Oligoclonal and polyclonal CD4 and CD8 lymphocytes in aplastic anemia and paroxysmal nocturnal hemoglobinuria measured by Vbeta CDR3 spectratyping and flow cytometry
Blood,
June 17, 2002;
100(1):
178 - 183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bellucci, E. P. Alyea, E. Weller, A. Chillemi, E. Hochberg, C. J. Wu, C. Canning, R. Schlossman, R. J. Soiffer, K. C. Anderson, et al.
Immunologic effects of prophylactic donor lymphocyte infusion after allogeneic marrow transplantation for multiple myeloma
Blood,
May 29, 2002;
99(12):
4610 - 4617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kook, A. M. Risitano, W. Zeng, M. Wlodarski, C. Lottemann, R. Nakamura, J. Barrett, N. S. Young, and J. P. Maciejewski
Changes in T-cell receptor VB repertoire in aplastic anemia: effects of different immunosuppressive regimens
Blood,
May 15, 2002;
99(10):
3668 - 3675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Hazenberg, S. A. Otto, E. S. de Pauw, H. Roelofs, W. E. Fibbe, D. Hamann, and F. Miedema
T-cell receptor excision circle and T-cell dynamics after allogeneic stem cell transplantation are related to clinical events
Blood,
May 1, 2002;
99(9):
3449 - 3453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bacci, C. Montagnoli, K. Perruccio, S. Bozza, R. Gaziano, L. Pitzurra, A. Velardi, C. F. d'Ostiani, J. E. Cutler, and L. Romani
Dendritic Cells Pulsed with Fungal RNA Induce Protective Immunity to Candida albicans in Hematopoietic Transplantation
J. Immunol.,
March 15, 2002;
168(6):
2904 - 2913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Talvensaari, E. Clave, C. Douay, C. Rabian, L. Garderet, M. Busson, F. Garnier, D. Douek, E. Gluckman, D. Charron, et al.
A broad T-cell repertoire diversity and an efficient thymic function indicate a favorable long-term immune reconstitution after cord blood stem cell transplantation
Blood,
February 15, 2002;
99(4):
1458 - 1464.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. P. Hochberg, A. C. Chillemi, C. J. Wu, D. Neuberg, C. Canning, K. Hartman, E. P. Alyea, R. J. Soiffer, S. A. Kalams, and J. Ritz
Quantitation of T-cell neogenesis in vivo after allogeneic bone marrow transplantation in adults
Blood,
August 15, 2001;
98(4):
1116 - 1121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Volpi, K. Perruccio, A. Tosti, M. Capanni, L. Ruggeri, S. Posati, F. Aversa, A. Tabilio, L. Romani, M. F. Martelli, et al.
Postgrafting administration of granulocyte colony-stimulating factor impairs functional immune recovery in recipients of human leukocyte antigen haplotype-mismatched hematopoietic transplants
Blood,
April 15, 2001;
97(8):
2514 - 2521.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mencacci, K. Perruccio, A. Bacci, E. Cenci, R. Benedetti, M. F. Martelli, F. Bistoni, R. Coffman, A. Velardi, and L. Romani
Defective antifungal T-helper 1 (TH1) immunity in a murine model of allogeneic T-cell-depleted bone marrow transplantation and its restoration by treatment with TH2 cytokine antagonists
Blood,
March 1, 2001;
97(5):
1483 - 1490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
