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Blood, Vol. 95 No. 12 (June 15), 2000:
pp. 4004-4007
BRIEF REPORT
Megakaryocyte dense granule components are sorted in
multivesicular bodies
Tayebeh Youssefian and
Elisabeth M. Cramer
From the INSERM U 474, Hôpital Cochin-Port-Royal, Paris,
France.
Recent studies suggest that multivesicular bodies are an
intermediate stage in the formation of  -granules. In contrast, the kinetics and mode of appearance of dense granules during megakaryocytic maturation has remained poorly understood. Immunoelectron microscopy was used to monitor the appearance of dense granular markers
(granulophysin and serotonin) on cryosections of human megakaryocytes
(MKs) cultured from CD34+ precursors. The monitoring was
done on days 8 and 13 of culture. The data suggest that dense granules
appear in immature MKs early during their maturation, concomitantly
with -granule formation. In MKs of intermediary maturation stage,
granulophysin was mainly localized within dense granules and
multivesicular bodies (MVBs), which were also labeled for serotonin.
This study provides evidence that granulophysin is a dense granule
marker in human MKs and that MVBs are an intermediary stage of dense
granule maturation and probably constitute a sorting compartment
between -granules and dense granules.
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