|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 95 No. 2 (January 15), 2000:
pp. 592-601
VCAM-1 is more effective than MAdCAM-1 in supporting eosinophil
rolling under conditions of shear flow
P. Sriramarao,
Richard G. DiScipio,
Ronald R. Cobb,
Myron Cybulsky,
Greg Stachnick,
Diego Castaneda,
Mariano Elices, and
David H. Broide
From the Laboratory of Immunology and Vascular Biology, La Jolla
Institute for Experimental Medicine La Jolla, CA; Cytel Corporation, La
Jolla, CA; Department of Biology, Tanabe Research Laboratories, San
Diego, CA; Department of Laboratory Medicine and Pathobiology,
University of Toronto, Toronto, Ontario; and Department of
Medicine, University of California San Diego, La Jolla, CA.
The ability of the  4 integrin counterligands vascular cell
adhesion molecule (VCAM)-1 or mucosal addressin (MAd)CAM-1 to support eosinophil rolling or firm adhesion under conditions of physiologic flow has not been delineated. Using a parallel plate flow
chamber in vitro and intravital microscopy in vivo, we demonstrate that
eosinophil rolling and adhesion on VCAM-1 is mediated by both 4 1
and 47 integrins. Eosinophils rolled equally efficiently on both
VCAM-1 2 domain and VCAM-1 7 domain, suggesting that the N-terminal 2 domains of VCAM-1 are sufficient to support eosinophil rolling under
conditions of flow. Furthermore, activation of the eosinophil 1
integrin with monoclonal antibody (mAb) 8A2 resulted in both resistance
to shear stress-induced detachment from VCAM-1 in vitro and in stable
arrest of rolling eosinophils on interleukin (IL)-1-stimulated
venules in vivo. Eosinophils rolled less efficiently on MAdCAM-1- than
on VCAM-1-coated coverslips under conditions of flow. However,
eosinophils firmly adhered as efficiently to MAdCAM-1 as to VCAM-1.
Overall, these results demonstrate that both VCAM-1 and MAdCAM-1 can
support eosinophil firm adhesion under conditions of flow. In contrast,
VCAM-1 is significantly more efficient than MAdCAM-1 in supporting
eosinophil rolling under conditions of flow.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. J. Tumes, A. Connolly, and L. A. Dent
Expression of survivin in lung eosinophils is associated with pathology in a mouse model of allergic asthma
Int. Immunol.,
June 1, 2009;
21(6):
633 - 644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. Barthel, M. W. Johansson, D. M. McNamee, and D. F. Mosher
Roles of integrin activation in eosinophil function and the eosinophilic inflammation of asthma
J. Leukoc. Biol.,
January 1, 2008;
83(1):
1 - 12.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Khaldoyanidi, V. V. Glinsky, L. Sikora, A. B. Glinskii, V. V. Mossine, T. P. Quinn, G. V. Glinsky, and P. Sriramarao
MDA-MB-435 Human Breast Carcinoma Cell Homo- and Heterotypic Adhesion under Flow Conditions Is Mediated in Part by Thomsen-Friedenreich Antigen-Galectin-3 Interactions
J. Biol. Chem.,
January 31, 2003;
278(6):
4127 - 4134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Y. Larbi, J. P. Dangerfield, F. J. Culley, D. Marshall, D. O. Haskard, P. J. Jose, T. J. Williams, and S. Nourshargh
P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses
J. Leukoc. Biol.,
January 1, 2003;
73(1):
65 - 73.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Broide
Fast Flowing Eosinophils . Signals for Stopping and Stepping Out of Blood Vessels
Am. J. Respir. Cell Mol. Biol.,
June 1, 2002;
26(6):
637 - 640.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Miller, K.-L. P. Sung, W. A. Muller, J. Y. Cho, M. Roman, D. Castaneda, J. Nayar, T. Condon, J. Kim, P. Sriramarao, et al.
Eosinophil Tissue Recruitment to Sites of Allergic Inflammation in the Lung Is Platelet Endothelial Cell Adhesion Molecule Independent
J. Immunol.,
August 15, 2001;
167(4):
2292 - 2297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Y. Larbi, A. R. Allen, F. W. K. Tam, D. O. Haskard, R. R. Lobb, P. M. R. Silva, and S. Nourshargh
VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration
Blood,
November 15, 2000;
96(10):
3601 - 3609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Tachimoto, M. M. Burdick, S. A. Hudson, M. Kikuchi, K. Konstantopoulos, and B. S. Bochner
CCR3-Active Chemokines Promote Rapid Detachment of Eosinophils from VCAM-1 In Vitro
J. Immunol.,
September 1, 2000;
165(5):
2748 - 2754.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
