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Blood, Vol. 95 No. 2 (January 15), 2000:
pp. 592-601
VCAM-1 is more effective than MAdCAM-1 in supporting eosinophil
rolling under conditions of shear flow
P. Sriramarao,
Richard G. DiScipio,
Ronald R. Cobb,
Myron Cybulsky,
Greg Stachnick,
Diego Castaneda,
Mariano Elices, and
David H. Broide
From the Laboratory of Immunology and Vascular Biology, La Jolla
Institute for Experimental Medicine La Jolla, CA; Cytel Corporation, La
Jolla, CA; Department of Biology, Tanabe Research Laboratories, San
Diego, CA; Department of Laboratory Medicine and Pathobiology,
University of Toronto, Toronto, Ontario; and Department of
Medicine, University of California San Diego, La Jolla, CA.
The ability of the  4 integrin counterligands vascular cell
adhesion molecule (VCAM)-1 or mucosal addressin (MAd)CAM-1 to support eosinophil rolling or firm adhesion under conditions of physiologic flow has not been delineated. Using a parallel plate flow
chamber in vitro and intravital microscopy in vivo, we demonstrate that
eosinophil rolling and adhesion on VCAM-1 is mediated by both 4 1
and 47 integrins. Eosinophils rolled equally efficiently on both
VCAM-1 2 domain and VCAM-1 7 domain, suggesting that the N-terminal 2 domains of VCAM-1 are sufficient to support eosinophil rolling under
conditions of flow. Furthermore, activation of the eosinophil 1
integrin with monoclonal antibody (mAb) 8A2 resulted in both resistance
to shear stress-induced detachment from VCAM-1 in vitro and in stable
arrest of rolling eosinophils on interleukin (IL)-1-stimulated
venules in vivo. Eosinophils rolled less efficiently on MAdCAM-1- than
on VCAM-1-coated coverslips under conditions of flow. However,
eosinophils firmly adhered as efficiently to MAdCAM-1 as to VCAM-1.
Overall, these results demonstrate that both VCAM-1 and MAdCAM-1 can
support eosinophil firm adhesion under conditions of flow. In contrast,
VCAM-1 is significantly more efficient than MAdCAM-1 in supporting
eosinophil rolling under conditions of flow.
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