|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 95 No. 5 (March 1), 2000:
pp. 1608-1615
Spatial associations of centromeres in the nuclei of hematopoietic
cells: evidence for cell-type-specific organizational
patterns
Isabel Alcobia,
Rui Dilão, and
Leonor Parreira
From the Institute of Histology and Embryology, Lisbon Medical
School, and the Nonlinear Dynamics Group, IST Department of Physics,
Lisbon, Portugal.
It is believed that the 3-dimensional organization of centromeric
heterochromatin in interphase may be of functional relevance as an
epigenetic mechanism for the regulation of gene expression. Accordingly, a likely possibility is that the centromeres that spatially associate into the heterochromatic structures (chromocenters) observed in the G1 phase of the cell cycle will differ in different cells. We sought to address this issue using, as a model, the chromocenters observed in quiescent normal human hematopoietic cells
and primary fibroblasts. To do this, we analyzed the spatial relationships among different human centromeres in 3-D preserved cells
using nonisotopic in situ hybridization and confocal microscopy. We
showed quantitatively that chromocenters in all cell types do indeed
represent nonrandom spatial associations of certain centromeres.
Furthermore, the observed patterns of centromere association indicate
that the chromocenters in these cell types are made of different
combinations of specific centromeres, that hematopoietic cells are
strikingly different from fibroblasts as to the composition of their
chromocenters and that centromeres in peripheral blood cells appear to
aggregate into distinct "myeloid" (present in monocytes and
granulocytes) and "lymphoid" (present in lymphocytes) spatial
patterns. These findings support the idea that the chromocenters formed
in the nucleus of quiescent hematopoietic cells might represent
heterochromatic nuclear compartments involved in the regulation of
cell-type-specific gene expression, further suggesting that the spatial
arrangement of centromeric heterochromatin in interphase is
ontogenically determined during hematopoietic differentiation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E J HALL and D J BRENNER
Cancer risks from diagnostic radiology
Br. J. Radiol.,
May 1, 2008;
81(965):
362 - 378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Finch, G. Fonseka, D. Ioannou, N. Hickson, Z. Barclay, K. Chatzimeletiou, A. Mantzouratou, A. Handyside, J. Delhanty, and D. K. Griffin
Nuclear organisation in totipotent human nuclei and its relationship to chromosomal abnormality
J. Cell Sci.,
March 1, 2008;
121(5):
655 - 663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Terranova, C. F. Pereira, C. Du Roure, M. Merkenschlager, and A. G. Fisher
Acquisition and extinction of gene expression programs are separable events in heterokaryon reprogramming
J. Cell Sci.,
May 15, 2006;
119(10):
2065 - 2072.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Vigodner, T. Ishikawa, P. N. Schlegel, and P. L. Morris
SUMO-1, human male germ cell development, and the androgen receptor in the testis of men with normal and abnormal spermatogenesis
Am J Physiol Endocrinol Metab,
May 1, 2006;
290(5):
E1022 - E1033.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wen, S. Huang, S. D. Pack, X. Yu, S. J. Brandt, and C. T. Noguchi
Tal1/SCL Binding to Pericentromeric DNA Represses Transcription
J. Biol. Chem.,
April 1, 2005;
280(13):
12956 - 12966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Arney and A. G. Fisher
Epigenetic aspects of differentiation
J. Cell Sci.,
September 1, 2004;
117(19):
4355 - 4363.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Guenatri, D. Bailly, C. Maison, and G. Almouzni
Mouse centric and pericentric satellite repeats form distinct functional heterochromatin
J. Cell Biol.,
August 16, 2004;
166(4):
493 - 505.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. N. Cornforth, K. M. Greulich-Bode, B. D. Loucas, J. Arsuaga, M. Vazquez, R. K. Sachs, M. Bruckner, M. Molls, P. Hahnfeldt, L. Hlatky, et al.
Chromosomes are predominantly located randomly with respect to each other in interphase human cells
J. Cell Biol.,
October 28, 2002;
159(2):
237 - 244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Carvalho, H. M. Pereira, J. Ferreira, C. Pina, D. Mendonca, A. C. Rosa, and M. Carmo-Fonseca
Chromosomal G-dark Bands Determine the Spatial Organization of Centromeric Heterochromatin in the Nucleus
Mol. Biol. Cell,
November 1, 2001;
12(11):
3563 - 3572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
