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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fouchet, C. Articles by Lopez, C. Search for Related Content PubMed PubMed Citation Articles by Fouchet, C. Articles by Lopez, C. Related Collections Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 95 No. 5 (March 1), 2000: pp. 1819-1826 A study of the coregulation and tissue specificity of XG and MIC2 gene expression in eukaryotic cells Claude Fouchet, Pierre Gane, Martine Huet, Marc Fellous, Philippe Rouger, George Banting, Jean-Pierre Cartron, and Claude Lopez From Inserm U76, Institut National de la Transfusion Sanguine, and Institut Pasteur, Paris, France, and the Department of Biochemistry, University of Bristol, Bristol, United Kingdom. CD99, the product of the MIC2 gene, exhibits an erythroid-specific quantitative polymorphism coregulated with the polymorphism of the XG blood group gene. As a preliminary study of this phenomenon, human XG and CD99 recombinant proteins were expressed in murine RAG cells and analyzed by flow cytometry. Both proteins were expressed independently and at a similar level in single and double transfectants. Immunoprecipitation and Western blot analysis, using the murine monoclonal antibodies NBL-1 and 12E7, revealed species of 26 kd (XG) and 32 kd (CD99), respectively. A putative 28-kd intracellular precursor of CD99 was also detected, as was a 26-kd species after neuraminidase treatment of CD99-expressing cells. No evidence of association or complex formation between XG and CD99 proteins could be proven, either on transfected RAG cells or on human erythrocytes. These results were confirmed using somatic hybrids between single transfectants. These findings suggest that the phenotypic relationship between XG and CD99 is mostly regulated at the transcriptional level, but they do not formally exclude some posttranscriptional effect. Studies on the tissue specificity of XG expression showed that surface expression of the XG protein could not be restored in somatic hybrids between B-lymphoblastoid cell lines from Xg(a+) persons and fibroblasts (RAG) or erythroid (MEL) cells. RT-PCR analysis of the transcripts revealed the existence of an XG mRNA in each cell line, suggesting that the tissue-specific regulation of cell surface XG expression occurs either at a quantitative transcriptional level or is a posttranscriptional event. By Northern blot analysis, XG transcripts were detected in erythroid tissues and several nonerythroid tissues. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I. Mouro-Chanteloup, A. M. D'Ambrosio, P. Gane, C. Le Van Kim, V. Raynal, D. Dhermy, J.-P. Cartron, and Y. Colin Cell-surface expression of RhD blood group polypeptide is posttranscriptionally regulated by the RhAG glycoprotein Blood, July 18, 2002; 100(3): 1038 - 1047. [Abstract] [Full Text] [PDF] K. Lee, P. Gane, F. Roudot-Thoraval, B. Godeau, D. Bachir, F. Bernaudin, J.-P. Cartron, F. Galacteros, and P. Bierling The nonexpression of CD36 on reticulocytes and mature red blood cells does not modify the clinical course of patients with sickle cell anemia Blood, August 15, 2001; 98(4): 966 - 971. [Abstract] [Full Text] [PDF]

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