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Blood, Vol. 95 No. 7 (April 1), 2000:
pp. 2284-2288
Expansion of hematopoietic stem cells in the developing liver of a
mouse embryo
Hideo Ema and
Hiromitsu Nakauchi
From the Department of Immunology, Institute of Basic Medical
Sciences, University of Tsukuba and CREST (JST), Tsukuba, Japan.
The activity of hematopoietic stem cells in the developing liver of
a C57BL/6 mouse embryo was quantified by a competitive repopulation
assay. Different doses of fetal liver cells at days 11 to 18 of
gestation were transplanted into irradiated mice together with
2 × 105 adult bone marrow cells. A long-term
repopulation in myeloid-, B-cell, and T-cell lineage by fetal liver
cells was evaluated at 20 weeks after transplantation. At day 12 of
gestation multilineage repopulating activity was first detected in the
liver as 50 repopulating units (RU) per liver. The number of RU per
liver increased 10-fold and 33-fold by day 14 and day 16 of gestation,
and decreased thereafter, suggesting a single wave of stem cell
development in the fetal liver. A limiting dilution analysis revealed
that the frequency of competitive repopulating units (CRU) in fetal
liver cells at day 12 of gestation was similar to that at day 16 of
gestation. Because of an increase of total fetal liver cell number, the
absolute number of CRU per liver from days 12 to 16 of gestation
increased 38-fold. Hence, the mean activity of stem cells (MAS) that is given by RU per CRU remained constant from days 12 to 16 of gestation. From these data we conclude that hematopoietic stem cells expand in the
fetal liver maintaining their level of repopulating potential.
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