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Blood, Vol. 95 No. 7 (April 1), 2000:
pp. 2446-2448
BRIEF REPORT
Genetic control of hematopoietic stem cell frequency in mice
is mostly cell autonomous
Christa E. Müller-Sieburg,
Rebecca H. Cho,
Hans B. Sieburg,
Sergey Kupriyanov, and
Roy Riblet
From the Sidney Kimmel Cancer Center; Department of Mathematics,
University of California, San Diego; The Burnham Institute, La Jolla;
and Torrey Pines Institute for Molecular Studies, San Diego, CA.
Previously we reported that the size of the stem cell compartment
(measured as LTC-IC) is 11-fold greater in DBA/2 than in C57BL/6 mice,
and we identified genes that regulate the size of the stem cell pool.
To determine whether stem cell intrinsic or extrinsic events account
for these differences, we created chimeras by aggregating morulae from
the strains C57BL/6 and DBA/2. In these chimeras stem cells of both
genotypes are exposed to a common mixed environment. Thus, an
equalization of stem cell frequencies is expected if stem cell
extrinsic effects dominate. Conversely, the parental ratio of LTC-IC
should be preserved if the regulation is stem cell autonomous. For each
chimera, individual LTC-IC were genotyped on the clonal levels by
analyzing their progeny. We found that most of the difference that
regulates the size of the stem cell compartment was intrinsic.
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