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Blood, Vol. 95 No. 8 (April 15), 2000:
pp. 2462-2470
Neutrophil polarity and locomotion are associated with surface
redistribution of leukosialin (CD43), an antiadhesive membrane
molecule
Stéphanie Seveau,
Hansuli Keller,
Frederick R. Maxfield,
Friedrich Piller, and
Lise Halbwachs-Mecarelli
From INSERM U507, Hôpital Necker, Paris, France; Institute of
Pathology, University of Bern, Switzerland; Department of Biochemistry,
Weill Medical College of Cornell University, New York, New York; Centre
de Biophysique Moléculaire, CNRS UPR 4301, Orléans, France.
This study analyzed the behavior of an antiadhesive membrane
molecule, CD43, in neutrophil polarization and locomotion. CD43 cross-linking by antibodies induced neutrophil locomotion, with CD43
molecules clustered at the uropod of polarized neutrophils. In
contrast, CD11b/CD18 cross-linking by antibodies did not affect either
cell polarization or locomotion. Stimulation of suspended or adherent
neutrophils with chemotactic peptide results in cell polarization and
locomotion and a concomitant redistribution of CD43 to the uropod. This
process is entirely reversible. The study also investigated which
actin-binding protein could be involved in CD43 lateral redistribution.
 -Actinin and moesin are preferentially adsorbed on Sepharose beads
bearing a recombinant CD43 intracellular domain. Analysis by
immunofluorescence confocal microscopy shows a codistribution of moesin
during CD43 lateral redistribution. By contrast, -actinin is located
at the leading edge, an area devoid of CD43. These results shed new
light on the role of CD43 membrane redistribution, which appears to be
directly related to neutrophil polarity and locomotion.
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