SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pierini, L. M. Articles by Maxfield, F. R. Search for Related Content PubMed PubMed Citation Articles by Pierini, L. M. Articles by Maxfield, F. R. Related Collections Focus on Hematology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 95 No. 8 (April 15), 2000: pp. 2471-2480 Oriented endocytic recycling of 51 in motile neutrophils Lynda M. Pierini, Moira A. Lawson, Robert J. Eddy, Bill Hendey, and Frederick R. Maxfield From the Department of Biochemistry, Weill Medical College of Cornell University, New York, NY; Department of Dairy and Food Science, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark; and Department of Pharmacology, Rush Medical College, Rush-Presbyterian-St. Luke's-Medical Center, Chicago, IL. During cell migration, integrin attachments to the substratum provide the means to generate the traction and force necessary to achieve locomotion. Once the cell has moved over these attachments, however, it is equally important that integrins detach from the substratum. The fate of integrins after detachment may include release from the cell, lateral diffusion across the cell surface, or endocytosis and redelivery to the cell surface. Polymorphonuclear neutrophils (PMNs) become stuck on the extracellular matrix proteins fibronectin and vitronectin when their intracellular free calcium concentration ([Ca++]i) is buffered. Taking advantage of this feature of PMN migration, we investigated the fate of integrins to differentiate among various models of migration. We demonstrate that 51, one of the fibronectin-binding integrins, is responsible for immobilization of [Ca++]i-buffered PMNs on fibronectin. We find that 5 and 1 are in endocytic vesicles in PMNs and that 5 colocalizes with a marker for an endocytic recycling compartment. When [Ca++]i is buffered, 5 and 1 become concentrated in clusters in the rear of the adherent cells, suggesting that [Ca++]i transients are required for 51 detachment from the substratum. Inhibition of 51 detachment by buffering [Ca++]i results in the depletion of 5 from both endocytic vesicles and the recycling compartment, providing compelling evidence that integrins are normally recycled by way of endocytosis and intracellular trafficking during cell migration. This model is further refined by our demonstration that the endocytic recycling compartment reorients to retain its localization just behind the leading lamella as PMNs migrate, indicating that membrane recycling during neutrophil migration has directionality. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Teckchandani, N. Toida, J. Goodchild, C. Henderson, J. Watts, B. Wollscheid, and J. A. Cooper Quantitative proteomics identifies a Dab2/integrin module regulating cell migration J. Cell Biol., July 13, 2009; 186(1): 99 - 111. [Abstract] [Full Text] [PDF] M. R. Cera, M. Fabbri, C. Molendini, M. Corada, F. Orsenigo, M. Rehberg, C. A. Reichel, F. Krombach, R. Pardi, and E. Dejana JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling J. Cell Sci., January 15, 2009; 122(2): 268 - 277. [Abstract] [Full Text] [PDF] M. S. Bretscher On the shape of migrating cells -- a `front-to-back' model J. Cell Sci., August 15, 2008; 121(16): 2625 - 2628. [Abstract] [Full Text] [PDF] K. M. Cooper, D. A. Bennin, and A. Huttenlocher The PCH Family Member Proline-Serine-Threonine Phosphatase-interacting Protein 1 Targets to the Leukocyte Uropod and Regulates Directed Cell Migration Mol. Biol. Cell, August 1, 2008; 19(8): 3180 - 3191. [Abstract] [Full Text] [PDF] F. Shi and J. Sottile Caveolin-1-dependent {beta}1 integrin endocytosis is a critical regulator of fibronectin turnover J. Cell Sci., July 15, 2008; 121(14): 2360 - 2371. [Abstract] [Full Text] [PDF] H. H. N. Yan, D. D. Mruk, W. M. Lee, and C. Y. Cheng Blood-testis barrier dynamics are regulated by testosterone and cytokines via their differential effects on the kinetics of protein endocytosis and recycling in Sertoli cells FASEB J, June 1, 2008; 22(6): 1945 - 1959. [Abstract] [Full Text] [PDF] V. Nechyporuk-Zloy, P. Dieterich, H. Oberleithner, C. Stock, and A. Schwab Dynamics of single potassium channel proteins in the plasma membrane of migrating cells Am J Physiol Cell Physiol, April 1, 2008; 294(4): C1096 - C1102. [Abstract] [Full Text] [PDF] L. Liu, B. He, W. M. Liu, D. Zhou, J. V. Cox, and X. A. Zhang Tetraspanin CD151 Promotes Cell Migration by Regulating Integrin Trafficking J. Biol. Chem., October 26, 2007; 282(43): 31631 - 31642. [Abstract] [Full Text] [PDF] A. L. Gonzalez, W. El-Bjeirami, J. L. West, L. V. McIntire, and C. W. Smith Transendothelial migration enhances integrin-dependent human neutrophil chemokinesis J. Leukoc. Biol., March 1, 2007; 81(3): 686 - 695. [Abstract] [Full Text] [PDF] T. Pellinen and J. Ivaska Integrin traffic. J. Cell Sci., September 15, 2006; 119(Pt 18): 3723 - 3731. [Abstract] [Full Text] [PDF] N. L. Prigozhina and C. M. Waterman-Storer Decreased polarity and increased random motility in PtK1 epithelial cells correlate with inhibition of endosomal recycling. J. Cell Sci., September 1, 2006; 119(Pt 17): 3571 - 3582. [Abstract] [Full Text] [PDF] N. E. Winterwood, A. Varzavand, M. N. Meland, L. K. Ashman, and C. S. Stipp A Critical Role for Tetraspanin CD151 in {alpha}3beta1 and {alpha}6beta4 Integrin-dependent Tumor Cell Functions on Laminin-5 Mol. Biol. Cell, June 1, 2006; 17(6): 2707 - 2721. [Abstract] [Full Text] [PDF] M. Fabbri, S. Di Meglio, M. C. Gagliani, E. Consonni, R. Molteni, J. R. Bender, C. Tacchetti, and R. Pardi Dynamic Partitioning into Lipid Rafts Controls the Endo-Exocytic Cycle of the {alpha}L/{beta}2 Integrin, LFA-1, during Leukocyte Chemotaxis Mol. Biol. Cell, December 1, 2005; 16(12): 5793 - 5803. [Abstract] [Full Text] [PDF] F. Balzac, M. Avolio, S. Degani, I. Kaverina, M. Torti, L. Silengo, J. V. Small, and S. F. Retta E-cadherin endocytosis regulates the activity of Rap1: a traffic light GTPase at the crossroads between cadherin and integrin function J. Cell Sci., October 15, 2005; 118(20): 4765 - 4783. [Abstract] [Full Text] [PDF] Q. Tong, E. V. Vassilieva, A. I. Ivanov, Z. Wang, G. T. Brown, C. A. Parkos, and A. Nusrat Interferon-{gamma} Inhibits T84 Epithelial Cell Migration by Redirecting Transcytosis of {beta}1 Integrin from the Migrating Leading Edge J. Immunol., September 15, 2005; 175(6): 4030 - 4038. [Abstract] [Full Text] [PDF] A. D. Doyle and J. Lee Cyclic changes in keratocyte speed and traction stress arise from Ca2+-dependent regulation of cell adhesiveness J. Cell Sci., January 15, 2005; 118(2): 369 - 379. [Abstract] [Full Text] [PDF] K.-P. Lim and W. Hong Human Nischarin/Imidazoline Receptor Antisera-selected Protein Is Targeted to the Endosomes by a Combined Action of a PX Domain and a Coiled-coil Region J. Biol. Chem., December 24, 2004; 279(52): 54770 - 54782. [Abstract] [Full Text] [PDF] A. NAVARRO, B. ANAND-APTE, and M.-O. PARAT A role for caveolae in cell migration FASEB J, December 1, 2004; 18(15): 1801 - 1811. [Abstract] [Full Text] [PDF] L. R. Strachan and M. L. Condic Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility J. Cell Biol., November 8, 2004; 167(3): 545 - 554. [Abstract] [Full Text] [PDF] S. C. Frasch, P. M. Henson, K. Nagaosa, M. B. Fessler, N. Borregaard, and D. L. Bratton Phospholipid Flip-Flop and Phospholipid Scramblase 1 (PLSCR1) Co-localize to Uropod Rafts in Formylated Met-Leu-Phe-stimulated Neutrophils J. Biol. Chem., April 23, 2004; 279(17): 17625 - 17633. [Abstract] [Full Text] [PDF] K. S. K. Uchida and S. Yumura Dynamics of novel feet of Dictyostelium cells during migration J. Cell Sci., March 15, 2004; 117(8): 1443 - 1455. [Abstract] [Full Text] [PDF] M. Kabir-Salmani, S. Shiokawa, Y. Akimoto, K. Sakai, and M. Iwashita The role of {alpha}5{beta}1-integrin in the IGF-I-induced migration of extravillous trophoblast cells during the process of implantation Mol. Hum. Reprod., February 1, 2004; 10(2): 91 - 97. [Abstract] [Full Text] [PDF] M. Prigent, T. Dubois, G. Raposo, V. Derrien, D. Tenza, C. Rosse, J. Camonis, and P. Chavrier ARF6 controls post-endocytic recycling through its downstream exocyst complex effector J. Cell Biol., December 8, 2003; 163(5): 1111 - 1121. [Abstract] [Full Text] [PDF] L. S. Price, M. Langeslag, J. P. t. Klooster, P. L. Hordijk, K. Jalink, and J. G. Collard Calcium Signaling Regulates Translocation and Activation of Rac J. Biol. Chem., October 10, 2003; 278(41): 39413 - 39421. [Abstract] [Full Text] [PDF] M. A. Lokuta, P. A. Nuzzi, and A. Huttenlocher Calpain regulates neutrophil chemotaxis PNAS, April 1, 2003; 100(7): 4006 - 4011. [Abstract] [Full Text] [PDF] L. M. Pierini, R. J. Eddy, M. Fuortes, S. Seveau, C. Casulo, and F. R. Maxfield Membrane Lipid Organization Is Critical for Human Neutrophil Polarization J. Biol. Chem., March 14, 2003; 278(12): 10831 - 10841. [Abstract] [Full Text] [PDF] H. Shinji, K. Seki, A. Tajima, A. Uchida, and S. Masuda Fibronectin Bound to the Surface of Staphylococcus aureus Induces Association of Very Late Antigen 5 and Intracellular Signaling Factors with Macrophage Cytoskeleton Infect. Immun., January 1, 2003; 71(1): 140 - 146. [Abstract] [Full Text] [PDF] A. L. Kindzelskii and H. R. Petty Intracellular Calcium Waves Accompany Neutrophil Polarization, Formylmethionylleucylphenylalanine Stimulation, and Phagocytosis: A High Speed Microscopy Study J. Immunol., January 1, 2003; 170(1): 64 - 72. [Abstract] [Full Text] [PDF] R. J. Eddy, L. M. Pierini, and F. R. Maxfield Microtubule Asymmetry during Neutrophil Polarization and Migration Mol. Biol. Cell, December 1, 2002; 13(12): 4470 - 4483. [Abstract] [Full Text] [PDF] J. E. Casanova Epithelial Cell Cytoskeleton and Intracellular Trafficking: V. Confluence of membrane trafficking and motility in epithelial cell models Am J Physiol Gastrointest Liver Physiol, November 1, 2002; 283(5): G1015 - G1019. [Abstract] [Full Text] [PDF] K. Thelen, V. Kedar, A. K. Panicker, R.-S. Schmid, B. R. Midkiff, and P. F. Maness The Neural Cell Adhesion Molecule L1 Potentiates Integrin-Dependent Cell Migration to Extracellular Matrix Proteins J. Neurosci., June 15, 2002; 22(12): 4918 - 4931. [Abstract] [Full Text] [PDF] D. Sheff, L. Pelletier, C. B. O'Connell, G. Warren, and I. Mellman Transferrin receptor recycling in the absence of perinuclear recycling endosomes J. Cell Biol., March 4, 2002; 156(5): 797 - 804. [Abstract] [Full Text] [PDF] L. Kolesnikova, H. Bugany, H.-D. Klenk, and S. Becker VP40, the Matrix Protein of Marburg Virus, Is Associated with Membranes of the Late Endosomal Compartment J. Virol., February 15, 2002; 76(4): 1825 - 1838. [Abstract] [Full Text] [PDF] R. A. Worthylake, S. Lemoine, J. M. Watson, and K. Burridge RhoA is required for monocyte tail retraction during transendothelial migration J. Cell Biol., July 9, 2001; 154(1): 147 - 160. [Abstract] [Full Text] [PDF] C. M. Laukaitis, D. J. Webb, K. Donais, and A. F. Horwitz Differential Dynamics of {alpha}5 Integrin, Paxillin, and {alpha}-Actinin during Formation and Disassembly of Adhesions in Migrating Cells J. Cell Biol., June 25, 2001; 153(7): 1427 - 1440. [Abstract] [Full Text] [PDF] A. Schwab Function and spatial distribution of ion channels and transporters in cell migration Am J Physiol Renal Physiol, May 1, 2001; 280(5): F739 - F747. [Abstract] [Full Text] [PDF] F. Berditchevski Complexes of tetraspanins with integrins: more than meets the eye J. Cell Sci., January 12, 2001; 114(23): 4143 - 4151. [Abstract] [Full Text] [PDF] E. M. Neuhaus and T. Soldati A Myosin I Is Involved in Membrane Recycling from Early Endosomes J. Cell Biol., September 4, 2000; 150(5): 1013 - 1026. [Abstract] [Full Text] [PDF] I. Mellman Quo Vadis: Polarized Membrane Recycling in Motility and Phagocytosis J. Cell Biol., May 1, 2000; 149(3): 529 - 530. [Full Text] [PDF] S. O'Neill, A. Robinson, A. Deering, M. Ryan, D. J. Fitzgerald, and N. Moran The Platelet Integrin alpha IIbbeta 3 Has an Endogenous Thiol Isomerase Activity J. Biol. Chem., November 17, 2000; 275(47): 36984 - 36990. [Abstract] [Full Text] [PDF] D. Sheff, L. Pelletier, C. B. O'Connell, G. Warren, and I. Mellman Transferrin receptor recycling in the absence of perinuclear recycling endosomes J. Cell Biol., March 4, 2002; 156(5): 797 - 804. [Abstract] [Full Text] [PDF] C. Ballestrem, B. Hinz, B. A. Imhof, and B. Wehrle-Haller Marching at the front and dragging behind: differential {alpha}V{beta}3-integrin turnover regulates focal adhesion behavior J. Cell Biol., December 24, 2001; 155(7): 1319 - 1332. [Abstract] [Full Text] [PDF]

	Copyright © 2000 by American Society of Hematology         Online ISSN: 1528-0020