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Blood, Vol. 95 No. 8 (April 15), 2000:
pp. 2543-2551
GATA-1 interacts with the myeloid PU.1 transcription factor and
represses PU.1-dependent transcription
Claus Nerlov,
Erich Querfurth,
Holger Kulessa, and
Thomas Graf
From the Laboratory of Gene Therapy Research, Copenhagen University
Hospital Copenhagen, Denmark; the European Molecular Biology
Laboratory, Heidelberg, Germany; and the Albert Einstein College of
Medicine, Bronx, NY.
The GATA-1 transcription factor is capable of suppressing the
myeloid gene expression program when ectopically expressed in myeloid
cells. We examined the ability of GATA-1 to repress the expression and
function of the PU.1 transcription factor, a central regulator of
myeloid differentiation. We found that GATA-1 is capable of suppressing
the myeloid phenotype without interfering with PU.1 gene expression,
but instead was capable of inhibiting the activity of the PU.1 protein
in a dose-dependent manner. This inhibition was independent of the
ability of GATA-1 to bind DNA, suggesting that it is mediated by
protein-protein interaction. We examined the ability of PU.1 to
interact with GATA-1 and found a direct interaction between the PU.1
ETS domain and the C-terminal finger region of GATA-1. Replacing the
PU.1 ETS domain with the GAL4 DNA-binding domain removed the ability of
GATA-1 to inhibit PU.1 activity, indicating that the PU.1 DNA-binding
domain, rather than the transactivation domain, is the target for
GATA-1-mediated repression. We therefore propose that GATA-1 represses
myeloid gene expression, at least in part, through its ability to
directly interact with the PU.1 ETS domain and thereby interfere with
PU.1 function.
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|
|
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|
|
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|
|
|
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|
|
|
|
|
|
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106(5):
1519 - 1524.
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|
|
|
|
|
|
|
H. Iwasaki, C. Somoza, H. Shigematsu, E. A. Duprez, J. Iwasaki-Arai, S.-i. Mizuno, Y. Arinobu, K. Geary, P. Zhang, T. Dayaram, et al.
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106(5):
1590 - 1600.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Geng, P. Laslo, K. Barton, and C.-R. Wang
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175(2):
1022 - 1029.
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[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Okuno, G. Huang, F. Rosenbauer, E. K. Evans, H. S. Radomska, H. Iwasaki, K. Akashi, F. Moreau-Gachelin, Y. Li, P. Zhang, et al.
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25(7):
2832 - 2845.
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[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ezoe, I. Matsumura, K. Gale, Y. Satoh, J. Ishikawa, M. Mizuki, S. Takahashi, N. Minegishi, K. Nakajima, M. Yamamoto, et al.
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280(13):
13163 - 13170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Bakri, S. Sarrazin, U. P. Mayer, S. Tillmanns, C. Nerlov, A. Boned, and M. H. Sieweke
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105(7):
2707 - 2716.
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|
|
|
|
|
|
|
R. Ferreira, K. Ohneda, M. Yamamoto, and S. Philipsen
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25(4):
1215 - 1227.
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[PDF]
|
|
|
|
|
|
|
R. Shimizu, T. Kuroha, O. Ohneda, X. Pan, K. Ohneda, S. Takahashi, S. Philipsen, and M. Yamamoto
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24(24):
10814 - 10825.
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[Full Text]
[PDF]
|
|
|
|
|
|
|
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104(10):
3136 - 3147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-N. Bastie, N. Balitrand, F. Guidez, I. Guillemot, J. Larghero, C. Calabresse, C. Chomienne, and L. Delva
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18(11):
2685 - 2699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Viger, H. Taniguchi, N. M. Robert, and J. J. Tremblay
The 25th Volume: Role of the GATA Family of Transcription Factors in Andrology
J Androl,
July 1, 2004;
25(4):
441 - 452.
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Rosenbauer, K. Wagner, P. Zhang, K.-P. Knobeloch, A. Iwama, and D. G. Tenen
pDP4, a novel glycoprotein secreted by mature granulocytes, is regulated by transcription factor PU.1
Blood,
June 1, 2004;
103(11):
4294 - 4301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rehli, H.-H. Niller, C. Ammon, S. Langmann, L. Schwarzfischer, R. Andreesen, and S. W. Krause
Transcriptional Regulation of CHI3L1, a Marker Gene for Late Stages of Macrophage Differentiation
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November 7, 2003;
278(45):
44058 - 44067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. S. Choe, F. Radparvar, I. Matushansky, N. Rekhtman, X. Han, and A. I. Skoultchi
Reversal of Tumorigenicity and the Block to Differentiation in Erythroleukemia Cells by GATA-1
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October 1, 2003;
63(19):
6363 - 6369.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Gombart, S. H. Kwok, K. L. Anderson, Y. Yamaguchi, B. E. Torbett, and H. P. Koeffler
Regulation of neutrophil and eosinophil secondary granule gene expression by transcription factors C/EBPepsilon and PU.1
Blood,
April 15, 2003;
101(8):
3265 - 3273.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cammenga, J. C. Mulloy, F. J. Berguido, D. MacGrogan, A. Viale, and S. D. Nimer
Induction of C/EBPalpha activity alters gene expression and differentiation of human CD34+ cells
Blood,
March 15, 2003;
101(6):
2206 - 2214.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Du, M. J. Stankiewicz, Y. Liu, Q. Xi, J. E. Schmitz, J. A. Lekstrom-Himes, and S. J. Ackerman
Novel Combinatorial Interactions of GATA-1, PU.1, and C/EBPepsilon Isoforms Regulate Transcription of the Gene Encoding Eosinophil Granule Major Basic Protein
J. Biol. Chem.,
November 1, 2002;
277(45):
43481 - 43494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. U. Mueller, T. Pabst, M. Osato, N. Asou, L. M. Johansen, M. D. Minden, G. Behre, W. Hiddemann, Y. Ito, and D. G. Tenen
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Blood,
July 18, 2002;
100(3):
998 - 1007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. A. Reddy, A. Iwama, G. Iotzova, M. Schulz, A. Elsasser, R. K. Vangala, D. G. Tenen, W. Hiddemann, and G. Behre
Granulocyte inducer C/EBPalpha inactivates the myeloid master regulator PU.1: possible role in lineage commitment decisions
Blood,
June 28, 2002;
100(2):
483 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Nishiyama, M. Hasegawa, M. Nishiyama, K. Takahashi, Y. Akizawa, T. Yokota, K. Okumura, H. Ogawa, and C. Ra
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J. Immunol.,
May 1, 2002;
168(9):
4546 - 4552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Graf
Differentiation plasticity of hematopoietic cells
Blood,
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99(9):
3089 - 3101.
[Full Text]
[PDF]
|
|
|
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