|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 95 No. 9 (May 1), 2000:
pp. 2954-2959
Importance of L-selectin-dependent
leukocyte-leukocyte interactions in human whole blood
Debra J. Mitchell,
Pauline Li,
Paul H. Reinhardt, and
Paul Kubes
From the Immunology Research Group, University of Calgary,
Calgary, Alberta, Canada.
The objective of this study was to investigate whether leukocytes
could be recruited by rolling leukocytes in a human whole blood model
system. In all experiments, either neutrophils, whole blood, or diluted
blood was perfused over immobilized E-selectin. With isolated
neutrophils (2 × 105/mL), the free-flowing neutrophils
were captured by attached neutrophils to form secondary interactions
that resulted in lines of rolling leukocytes. These secondary tethers
accounted for 50% to 60% of all interactions and were eliminated by
an L-selectin antibody, which also eliminated the lines of rolling
leukocytes. Perfusion of whole blood or diluted blood revealed no lines
of rolling leukocytes. The addition of red blood cells to isolated
neutrophils either in a 1000:1 or a 10:1 ratio also inhibited lines of
rolling leukocytes. Leukocytes were fluorescently labeled with
rhodamine-6G so that leukocyte-leukocyte interactions could be studied
in whole blood. A small number of secondary tethers (less than 20%)
occurred and could be reduced by more than 80% with an L-selectin
antibody. However, the overall impact on leukocyte recruitment was
negligible. Similar experiments were performed using murine whole blood
or isolated murine leukocytes. In the absence of red blood cells, murine leukocytes also formed lines of rolling leukocytes on
E-selectin, and secondary tethers accounted for 50% of total
interactions. However, when murine blood (diluted 1:5 with buffer) was
perfused over E-selectin, secondary tethers accounted for only 13% of
total interactions. These interactions were completely absent when
blood was used from L-selectin-deficient mice. These data demonstrate for the first time that the importance of L-selectin-dependent leukocyte-leukocyte interactions is greatly reduced in whole blood and
does not enhance overall recruitment of leukocytes in this physiologic milieu.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. Petri, M. Phillipson, and P. Kubes
The Physiology of Leukocyte Recruitment: An In Vivo Perspective
J. Immunol.,
May 15, 2008;
180(10):
6439 - 6446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Burdick and K. Konstantopoulos
Platelet-induced enhancement of LS174T colon carcinoma and THP-1 monocytoid cell adhesion to vascular endothelium under flow
Am J Physiol Cell Physiol,
August 1, 2004;
287(2):
C539 - C547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. B. Abbitt and G. B. Nash
Rheological properties of the blood influencing selectin-mediated adhesion of flowing leukocytes
Am J Physiol Heart Circ Physiol,
June 5, 2003;
285(1):
H229 - H240.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. St. Hill, S. R. Alexander, and B. Walcheck
Indirect capture augments leukocyte accumulation on P-selectin in flowing whole blood
J. Leukoc. Biol.,
April 1, 2003;
73(4):
464 - 471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Bowden, Z.-M. Ding, E. M. Donnachie, T. K. Petersen, L. H. Michael, C. M. Ballantyne, and A. R. Burns
Role of {alpha}4 Integrin and VCAM-1 in CD18-Independent Neutrophil Migration Across Mouse Cardiac Endothelium
Circ. Res.,
March 22, 2002;
90(5):
562 - 569.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. King and D. A. Hammer
Multiparticle adhesive dynamics: Hydrodynamic recruitment of rolling leukocytes
PNAS,
December 18, 2001;
98(26):
14919 - 14924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Jadhav, B. S. Bochner, and K. Konstantopoulos
Hydrodynamic Shear Regulates the Kinetics and Receptor Specificity of Polymorphonuclear Leukocyte-Colon Carcinoma Cell Adhesive Interactions
J. Immunol.,
November 15, 2001;
167(10):
5986 - 5993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. E. Eriksson, X. Xie, J. Werr, P. Thoren, and L. Lindbom
Importance of Primary Capture and L-Selectin-Dependent Secondary Capture in Leukocyte Accumulation in Inflammation and Atherosclerosis in Vivo
J. Exp. Med.,
July 16, 2001;
194(2):
205 - 218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Bowden, Z.-M. Ding, E. M. Donnachie, T. K. Petersen, L. H. Michael, C. M. Ballantyne, and A. R. Burns
Role of {alpha}4 Integrin and VCAM-1 in CD18-Independent Neutrophil Migration Across Mouse Cardiac Endothelium
Circ. Res.,
March 22, 2002;
90(5):
562 - 569.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
