|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 96 No. 1 (July 1), 2000:
pp. 145-148
The elusive factor Xa receptor: failure to detect transcripts that
correspond to the published sequence of EPR-1
Guido J. R. Zaman and
Edward M. Conway
From N.V. Organon, Research and Development, Oss, The Netherlands;
and Department of Medicine, Institute of Medical Science, University of
Toronto, Toronto, Canada.
The coagulation protease factor Xa induces cellular
responses implicated in cardiovascular and inflammatory disease.
Effector-cell protease receptor 1 (EPR-1) is a functionally
characterized receptor of factor Xa, and the EPR-1
complementary DNA (cDNA) was published. Remarkably, the cDNA encoding
an inhibitor of apoptosis, survivin, is reportedly identical to that of
EPR-1 except for a few nucleotide differences and its
orientation opposite to EPR-1. To isolate the EPR-1
cDNA and gene, we surveyed gene databases for expressed sequence tags
(ESTs) that could be derived from EPR-1. All ESTs with strong
homology to EPR-1/survivin were derived from survivin and could not encode EPR-1. By polymerase chain reaction and Southern blot hybridization, EPR-1 was not detectable in the human or
murine genome, but survivin was. Our data suggest that EPR-1 is
either highly cell-specific or the published EPR-1 cDNA
includes sequences from clones derived from survivin messenger
RNA. The means by which factor Xa mediates its cellular effects
requires further evaluation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K Borensztajn, M P Peppelenbosch, and C A Spek
Letter in response to 'Coagulation and fibrosis in chronic liver disease'
Gut,
November 1, 2009;
58(11):
1565 - 1566.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Monroe, M. Hoffman, and H. R. Roberts
Platelets and Thrombin Generation
Arterioscler Thromb Vasc Biol,
September 1, 2002;
22(9):
1381 - 1389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Camerer, H. Kataoka, M. Kahn, K. Lease, and S. R. Coughlin
Genetic Evidence That Protease-activated Receptors Mediate Factor Xa Signaling in Endothelial Cells
J. Biol. Chem.,
May 3, 2002;
277(18):
16081 - 16087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Riewald, V. V. Kravchenko, R. J. Petrovan, P. J. O'Brien, L. F. Brass, R. J. Ulevitch, and W. Ruf
Gene induction by coagulation factor Xa is mediated by activation of protease-activated receptor 1
Blood,
May 15, 2001;
97(10):
3109 - 3116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-L. Hung, E. S. Pollak, R. D. Kudaravalli, V. Arruda, K. Chu, and K. A. High
Regulation of human coagulation factor X gene expression by GATA-4 and the Sp family of transcription factors
Blood,
February 15, 2001;
97(4):
946 - 951.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J Silke and D. Vaux
Two kinds of BIR-containing protein - inhibitors of apoptosis, or required for mitosis
J. Cell Sci.,
January 5, 2001;
114(10):
1821 - 1827.
[Abstract]
[PDF]
|
|
|
|
|
