|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 96 No. 1 (July 1), 2000:
pp. 41-49
Expression and coreceptor activity of STRL33/Bonzo on primary
peripheral blood lymphocytes
Matthew Sharron,
Stefan Pöhlmann,
Ken Price,
Elias Lolis,
Monica Tsang,
Frank Kirchhoff,
Robert W. Doms, and
Benhur Lee
From the University of Pennsylvania, Department of Pathology and
Laboratory Medicine, Philadelphia, PA; the Institute for Clinical and
Molecular Virology, University of Erlangen-Nürnberg,
Schlo garten, Erlangen, Germany; R&D Systems,
Minneapolis, MN; and the Department of Pharmacology, Yale University,
New Haven, CT.
CCR5 and CXCR4 are the major coreceptors that mediate human
immunodeficiency virus 1 (HIV-1) infection, while most simian immunodeficiency virus (SIV) isolates use CCR5. A number of alternative coreceptors can also mediate infection of some virus strains in vitro,
although little is known about their in vivo relevance. Therefore, we characterized the expression pattern and coreceptor activity of one of these alternative coreceptors, STRL33/Bonzo, using a
newly developed monoclonal antibody. In addition to being highly
expressed (approximately 1000-7000 STRL33 ABS [antibody binding
sites]) on specific subsets of natural killer cells
(CD3 /CD16/low/CD56+ and
CD3/CD16low/CD56) and
CD19+ B lymphocytes (approximately 300-5000 STRL33 ABS),
STRL33 was expressed at levels sufficient to support virus infection on
freshly isolated, truly naive
CD4+/CD45RA+/CD62L+
cells (6000-11 000 ABS). STRL33 expression on peripheral blood mononuclear cells (PBMCs) was increased by mitogenic stimulation (OKT3/IL-2 [interleukin-2] had a greater effect than
phytohemaglutinin (PHA)/IL-2), but it was dramatically decreased
upon Ficoll purification. Infection of CCR5 human
peripheral blood lymphocytes (PBLs) showed that 2 different SIV
envelope (Env) proteins mediated entry into STRL33+
cells. More importantly, the preferential infection of
STRL33+ cells in CCR5 PBLs by an
R5/X4/STRL33 HIV-1 maternal isolate in the presence of a
potent CXCR4 antagonist (AMD3100) suggests that STRL33 can be used as a
coreceptor by HIV-1 on primary cells. Rhesus macaque (rh) STRL33 was
used less efficiently than human STRL33 by the majority of SIV Env
proteins tested despite similar levels of expression, thereby making it
less likely that STRL33 is a relevant coreceptor in the rhesus macaque
system. In summary, the expression pattern and coreceptor activity of
STRL33 suggest its involvement in trafficking of tumor-infiltrating
lymphocytes and indicate that STRL33 may be a relevant coreceptor in vivo.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. W.-P. Hong, S. Nguyen, S. Young, S. V. Su, and B. Lee
Identification of the Optimal DC-SIGN Binding Site on Human Immunodeficiency Virus Type 1 gp120
J. Virol.,
August 1, 2007;
81(15):
8325 - 8336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. E. Garcia, L. D. Truong, P. Li, P. Zhang, R. J. Johnson, C. B. Wilson, and L. Feng
Inhibition of CXCL16 Attenuates Inflammatory and Progressive Phases of Anti-Glomerular Basement Membrane Antibody-Associated Glomerulonephritis
Am. J. Pathol.,
May 1, 2007;
170(5):
1485 - 1496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Flint, T. von Hahn, J. Zhang, M. Farquhar, C. T. Jones, P. Balfe, C. M. Rice, and J. A. McKeating
Diverse CD81 Proteins Support Hepatitis C Virus Infection
J. Virol.,
November 15, 2006;
80(22):
11331 - 11342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Monceaux, L. Viollet, F. Petit, R. H. T. Fang, M.-C. Cumont, J. Zaunders, B. Hurtrel, and J. Estaquier
CD8+ T Cell Dynamics during Primary Simian Immunodeficiency Virus Infection in Macaques: Relationship of Effector Cell Differentiation with the Extent of Viral Replication
J. Immunol.,
June 1, 2005;
174(11):
6898 - 6908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Heydtmann, P. F. Lalor, J. A. Eksteen, S. G. Hubscher, M. Briskin, and D. H. Adams
CXC Chemokine Ligand 16 Promotes Integrin-Mediated Adhesion of Liver-Infiltrating Lymphocytes to Cholangiocytes and Hepatocytes within the Inflamed Human Liver
J. Immunol.,
January 15, 2005;
174(2):
1055 - 1062.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Kolokoltsov and R. A. Davey
Rapid and Sensitive Detection of Retrovirus Entry by Using a Novel Luciferase-Based Content-Mixing Assay
J. Virol.,
May 15, 2004;
78(10):
5124 - 5132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J.-Y. Ploquin, O. M. Diop, N. Sol-Foulon, L. Mortara, A. Faye, M. A. Soares, E. Nerrienet, R. Le Grand, Y. Van Kooyk, A. Amara, et al.
DC-SIGN from African Green Monkeys Is Expressed in Lymph Nodes and Mediates Infection in trans of Simian Immunodeficiency Virus SIVagm
J. Virol.,
January 15, 2004;
78(2):
798 - 810.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Veazey, P. J. Klasse, T. J. Ketas, J. D. Reeves, M. Piatak Jr., K. Kunstman, S. E. Kuhmann, P. A. Marx, J. D. Lifson, J. Dufour, et al.
Use of a Small Molecule CCR5 Inhibitor in Macaques to Treat Simian Immunodeficiency Virus Infection or Prevent Simian-Human Immunodeficiency Virus Infection
J. Exp. Med.,
November 17, 2003;
198(10):
1551 - 1562.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Forte, M.-E. Harmon, M. J. Pineda, and J. Overbaugh
Early- and Intermediate-Stage Variants of Simian Immunodeficiency Virus Replicate Efficiently in Cells Lacking CCR5
J. Virol.,
September 1, 2003;
77(17):
9723 - 9727.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Willey, J. D. Reeves, R. Hudson, K. Miyake, N. Dejucq, D. Schols, E. D. Clercq, J. Bell, A. McKnight, and P. R. Clapham
Identification of a Subset of Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus Strains Able To Exploit an Alternative Coreceptor on Untransformed Human Brain and Lymphoid Cells
J. Virol.,
June 1, 2003;
77(11):
6138 - 6152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Cilliers, J. Nhlapo, M. Coetzer, D. Orlovic, T. Ketas, W. C. Olson, J. P. Moore, A. Trkola, and L. Morris
The CCR5 and CXCR4 Coreceptors Are Both Used by Human Immunodeficiency Virus Type 1 Primary Isolates from Subtype C
J. Virol.,
April 1, 2003;
77(7):
4449 - 4456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. W.-P. Hong, K. B. Flummerfelt, A. de Parseval, K. Gurney, J. H. Elder, and B. Lee
Human Immunodeficiency Virus Envelope (gp120) Binding to DC-SIGN and Primary Dendritic Cells Is Carbohydrate Dependent but Does Not Involve 2G12 or Cyanovirin Binding Sites: Implications for Structural Analyses of gp120-DC-SIGN Binding
J. Virol.,
November 13, 2002;
76(24):
12855 - 12865.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. R. Clapham and A. McKnight
Cell surface receptors, virus entry and tropism of primate lentiviruses
J. Gen. Virol.,
August 1, 2002;
83(8):
1809 - 1829.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Reeves and R. W. Doms
Human immunodeficiency virus type 2
J. Gen. Virol.,
June 1, 2002;
83(6):
1253 - 1265.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. Soilleux, L. S. Morris, G. Leslie, J. Chehimi, Q. Luo, E. Levroney, J. Trowsdale, L. J. Montaner, R. W. Doms, D. Weissman, et al.
Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitro
J. Leukoc. Biol.,
March 1, 2002;
71(3):
445 - 457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Puffer, S. Pohlmann, A. L. Edinger, D. Carlin, M. D. Sanchez, J. Reitter, D. D. Watry, H. S. Fox, R. C. Desrosiers, and R. W. Doms
CD4 Independence of Simian Immunodeficiency Virus Envs Is Associated with Macrophage Tropism, Neutralization Sensitivity, and Attenuated Pathogenicity
J. Virol.,
February 22, 2002;
76(6):
2595 - 2605.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Lee, G. Leslie, E. Soilleux, U. O'Doherty, S. Baik, E. Levroney, K. Flummerfelt, W. Swiggard, N. Coleman, M. Malim, et al.
cis Expression of DC-SIGN Allows for More Efficient Entry of Human and Simian Immunodeficiency Viruses via CD4 and a Coreceptor
J. Virol.,
December 15, 2001;
75(24):
12028 - 12038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Baribaud, T. G. Edwards, M. Sharron, A. Brelot, N. Heveker, K. Price, F. Mortari, M. Alizon, M. Tsang, and R. W. Doms
Antigenically Distinct Conformations of CXCR4
J. Virol.,
October 1, 2001;
75(19):
8957 - 8967.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. R Clapham and A. McKnight
HIV-1 receptors and cell tropism
Br. Med. Bull.,
September 1, 2001;
58(1):
43 - 59.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Pöhlmann, F. Baribaud, B. Lee, G. J. Leslie, M. D. Sanchez, K. Hiebenthal-Millow, J. Münch, F. Kirchhoff, and R. W. Doms
DC-SIGN Interactions with Human Immunodeficiency Virus Type 1 and 2 and Simian Immunodeficiency Virus
J. Virol.,
May 15, 2001;
75(10):
4664 - 4672.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Wilbanks, S. C. Zondlo, K. Murphy, S. Mak, D. Soler, P. Langdon, D. P. Andrew, L. Wu, and M. Briskin
Expression Cloning of the STRL33/BONZO/TYMSTR Ligand Reveals Elements of CC, CXC, and CX3C Chemokines
J. Immunol.,
April 15, 2001;
166(8):
5145 - 5154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Pohlmann, E. J. Soilleux, F. Baribaud, G. J. Leslie, L. S. Morris, J. Trowsdale, B. Lee, N. Coleman, and R. W. Doms
DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans
PNAS,
February 27, 2001;
98(5):
2670 - 2675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Edinger, M. Ahuja, T. Sung, K. C. Baxter, B. Haggarty, R. W. Doms, and J. A. Hoxie
Characterization and Epitope Mapping of Neutralizing Monoclonal Antibodies Produced by Immunization with Oligomeric Simian Immunodeficiency Virus Envelope Protein
J. Virol.,
September 1, 2000;
74(17):
7922 - 7935.
[Abstract]
[Full Text]
|
|
|
| |
