|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 November 2000, Vol. 96, No. 10, pp. 3452-3458
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Binding of factor VIIa to tissue factor on human fibroblasts
leads to activation of phospholipase C and enhanced
PDGF-BB-stimulated chemotaxis
Agneta Siegbahn,
Matilda Johnell,
Charlotte Rorsman,
Mirella Ezban,
Carl-Henrik Heldin, and
Lars Rönnstrand
From the Department of Medical Sciences, Laboratory for
Coagulation Research, Clinical Chemistry, University Hospital, Uppsala,
Sweden; the Ludwig Institute for Cancer Research,
Biomedical Centre, Uppsala, Sweden; and Tissue
Factor/Factor VIIa Research, Health Care Discovery, Novo Nordisk A/S,
Maaloev, Denmark.
Tissue factor (TF) is the cellular receptor for factor FVIIa
(FVIIa), and the complex is the principal initiator of blood coagulation. The effects of FVIIa binding to TF on cell migration and
signal transduction of human fibroblasts, which express high amounts of
TF, were studied. Fibroblasts incubated with FVIIa migrated toward a
concentration gradient of PDGF-BB at approximately 100 times lower
concentration than do fibroblasts not ligated with FVIIa. Anti-TF
antibodies inhibited the increase in chemotaxis induced by FVIIa/TF.
Moreover, a pronounced suppression of chemotaxis induced by PDGF-BB was
observed with active site-inhibited FVIIa (FFR-FVIIa). The possibility
that hyperchemotaxis was induced by a putative generation of FXa and
thrombin activity was excluded. FVIIa/TF did not induce increased
levels of PDGF  -receptors on the cell surface. Thus, the
hyperchemotaxis was not a result of this mechanism. FVIIa induced the
production of inositol-1,4,5-trisphosphate to the same extent as
PDGF-BB; the effects of FVIIa and PDGF-BB were additive. FFR-FVIIa did
not induce any release of inositol-1,4,5,-trisphosphate. Thus, binding
of catalytically active FVIIa to TF can, independent of coagulation,
modulate cellular responses, such as chemotaxis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Siegbahn, M. Johnell, A. Nordin, M. Aberg, and T. Velling
TF/FVIIa Transactivate PDGFR to Regulate PDGF-BB Induced Chemotaxis in Different Cell Types: Involvement of Src And PLC
Arterioscler Thromb Vasc Biol,
January 1, 2008;
28(1):
135 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Nielsen-Preiss, M. P. Allen, M. Xu, D. A. Linseman, J. E. Pawlowski, R. J. Bouchard, B. C. Varnum, K. A. Heidenreich, and M. E. Wierman
Adhesion-Related Kinase Induction of Migration Requires Phosphatidylinositol-3-Kinase and Ras Stimulation of Rac Activity in Immortalized Gonadotropin-Releasing Hormone Neuronal Cells
Endocrinology,
June 1, 2007;
148(6):
2806 - 2814.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Malarstig, T. Tenno, N. Johnston, B. Lagerqvist, T. Axelsson, A.-C. Syvanen, L. Wallentin, and A. Siegbahn
Genetic Variations in the Tissue Factor Gene Are Associated With Clinical Outcome in Acute Coronary Syndrome and Expression Levels in Human Monocytes
Arterioscler Thromb Vasc Biol,
December 1, 2005;
25(12):
2667 - 2672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Pandolfi
C-reactive protein: A potential new molecular link between inflammation, thrombosis and vascular cell proliferation?
Cardiovasc Res,
October 1, 2005;
68(1):
3 - 4.
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Ott, C. Michaelis, M. Schuermann, B. Steppich, I. Seitz, M. Dewerchin, D. Zohlnhofer, R. Wessely, M. Rudelius, A. Schomig, et al.
Vascular Remodeling in Mice Lacking the Cytoplasmic Domain of Tissue Factor
Circ. Res.,
August 5, 2005;
97(3):
293 - 298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Belting, J. Ahamed, and W. Ruf
Signaling of the Tissue Factor Coagulation Pathway in Angiogenesis and Cancer
Arterioscler Thromb Vasc Biol,
August 1, 2005;
25(8):
1545 - 1550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. V. M. Rao and U. R. Pendurthi
Tissue Factor-Factor VIIa Signaling
Arterioscler Thromb Vasc Biol,
January 1, 2005;
25(1):
47 - 56.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Dorfleutner, E. Hintermann, T. Tarui, Y. Takada, and W. Ruf
Cross-talk of Integrin {alpha}3{beta}1 and Tissue Factor in Cell Migration
Mol. Biol. Cell,
October 1, 2004;
15(10):
4416 - 4425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Ahamed and W. Ruf
Protease-activated Receptor 2-dependent Phosphorylation of the Tissue Factor Cytoplasmic Domain
J. Biol. Chem.,
May 28, 2004;
279(22):
23038 - 23044.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. M. Hjortoe, L. C. Petersen, T. Albrektsen, B. B. Sorensen, P. L. Norby, S. K. Mandal, U. R. Pendurthi, and L. V. M. Rao
Tissue factor-factor VIIa-specific up-regulation of IL-8 expression in MDA-MB-231 cells is mediated by PAR-2 and results in increased cell migration
Blood,
April 15, 2004;
103(8):
3029 - 3037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W. Kopp, T. Holzenbein, S. Steiner, R. Marculescu, H. Bergmeister, D. Seidinger, I. Mosberger, C. Kaun, M. Cejna, R. Horvat, et al.
Inhibition of restenosis by tissue factor pathway inhibitor: in vivo and in vitro evidence for suppressed monocyte chemoattraction and reduced gelatinolytic activity
Blood,
March 1, 2004;
103(5):
1653 - 1661.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. B. Sorensen, L. V. M. Rao, D. Tornehave, S. Gammeltoft, and L. C. Petersen
Antiapoptotic effect of coagulation factor VIIa
Blood,
September 1, 2003;
102(5):
1708 - 1715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. H. Versteeg, M. P. Peppelenbosch, and C. A. Spek
Tissue factor signal transduction in angiogenesis
Carcinogenesis,
June 1, 2003;
24(6):
1009 - 1013.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Iakhiaev, U. R. Pendurthi, and L. V. M. Rao
Active Site Blockade of Factor VIIa Alters Its Intracellular Distribution
J. Biol. Chem.,
November 30, 2001;
276(49):
45895 - 45901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
