SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sahni, A. Articles by Francis, C. W. Search for Related Content PubMed PubMed Citation Articles by Sahni, A. Articles by Francis, C. W. Related Collections Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 December 2000, Vol. 96, No. 12, pp. 3772-3778 HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Vascular endothelial growth factor binds to fibrinogen and fibrin and stimulates endothelial cell proliferation Abha Sahni and Charles W. Francis From the Vascular Medicine Unit, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY. Vascular development and response to injury are regulated by several cytokines and growth factors including the members of the fibroblast growth factor and vascular endothelial cell growth factor (VEGF) families. Fibrinogen and fibrin are also important in these processes and affect many endothelial cell properties. Possible specific interactions between VEGF and fibrinogen that could play a role in coordinating vascular responses to injury are investigated. Binding studies using the 165 amino acid form of VEGF immobilized on Sepharose beads and soluble iodine 125 (125I)-labeled fibrinogen demonstrated saturable and specific binding. Scatchard analysis indicated 2 classes of binding sites with dissociation constants (Kds) of 5.9 and 462 nmol/L. The maximum molar binding ratio of VEGF:fibrinogen was 3.8:1. Further studies characterized binding to fibrin using 125I-labeled VEGF- and Sepharose-immobilized fibrin monomer. These also demonstrated specific and saturable binding with 2 classes of sites having Kds of 0.13 and 97 nmol/L and a molar binding ratio of 3.6:1. Binding to polymerized fibrin demonstrated one binding site with a Kd of 9.3 nmol/L. Binding of VEGF to fibrin(ogen) was independent of FGF-2, indicating that there are distinct binding sites for each angiogenic peptide. VEGF bound to soluble fibrinogen in medium and to surface immobilized fibrinogen or fibrin retained its capacity to support endothelial cell proliferation. VEGF binds specifically and saturably to fibrinogen and fibrin with high affinity, and this may affect the localization and activity of VEGF at sites of tissue injury. © 2000 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. M. Ghajar, X. Chen, J. W. Harris, V. Suresh, C. C. W. Hughes, N. L. Jeon, A. J. Putnam, and S. C. George The Effect of Matrix Density on the Regulation of 3-D Capillary Morphogenesis Biophys. J., March 1, 2008; 94(5): 1930 - 1941. [Abstract] [Full Text] [PDF] T. Hou, S. Ray, and A. R. Brasier The Functional Role of an Interleukin 6-inducible CDK9{middle dot}STAT3 Complex in Human {gamma}-Fibrinogen Gene Expression J. Biol. Chem., December 21, 2007; 282(51): 37091 - 37102. [Abstract] [Full Text] [PDF] A. T. Nurden Platelets and Tissue Remodeling: Extending the Role of the Blood Clotting System Endocrinology, July 1, 2007; 148(7): 3053 - 3055. [Full Text] [PDF] A. Sahni, A. A. Khorana, R. B. Baggs, H. Peng, and C. W. Francis FGF-2 binding to fibrin(ogen) is required for augmented angiogenesis Blood, January 1, 2006; 107(1): 126 - 131. [Abstract] [Full Text] [PDF] A. Sahni, S. K. Sahni, and C. W. Francis Endothelial Cell Activation by IL-1{beta} in the Presence of Fibrinogen Requires {alpha}V{beta}3 Arterioscler. Thromb. Vasc. Biol., October 1, 2005; 25(10): 2222 - 2227. [Abstract] [Full Text] [PDF] Y.-C. Huang, R. G. Dennis, L. Larkin, and K. Baar Rapid formation of functional muscle in vitro using fibrin gels J Appl Physiol, February 1, 2005; 98(2): 706 - 713. [Abstract] [Full Text] [PDF] A. Sahni and C. W. Francis Stimulation of endothelial cell proliferation by FGF-2 in the presence of fibrinogen requires {alpha}v{beta}3 Blood, December 1, 2004; 104(12): 3635 - 3641. [Abstract] [Full Text] [PDF] G. Bocci, S. Man, S. K. Green, G. Francia, J. M. L. Ebos, J. M. du Manoir, A. Weinerman, U. Emmenegger, L. Ma, P. Thorpe, et al. Increased Plasma Vascular Endothelial Growth Factor (VEGF) as a Surrogate Marker for Optimal Therapeutic Dosing of VEGF Receptor-2 Monoclonal Antibodies Cancer Res., September 15, 2004; 64(18): 6616 - 6625. [Abstract] [Full Text] [PDF] A. Sahni, M. Guo, S. K. Sahni, and C. W. Francis Interleukin-1{beta} but not IL-1{alpha} binds to fibrinogen and fibrin and has enhanced activity in the bound form Blood, July 15, 2004; 104(2): 409 - 414. [Abstract] [Full Text] [PDF] R. A. Adams, M. Passino, B. D. Sachs, T. Nuriel, and K. Akassoglou Fibrin Mechanisms and Functions in Nervous System Pathology Mol. Interv., June 1, 2004; 4(3): 163 - 176. [Abstract] [Full Text] [PDF] H. Peng, A. Sahni, P. Fay, S. Bellum, I. Prudovsky, T. Maciag, and C. W. Francis Identification of a binding site on human FGF-2 for fibrinogen Blood, March 15, 2004; 103(6): 2114 - 2120. [Abstract] [Full Text] [PDF] B. J. Rybarczyk, S. O. Lawrence, and P. J. Simpson-Haidaris Matrix-fibrinogen enhances wound closure by increasing both cell proliferation and migration Blood, December 1, 2003; 102(12): 4035 - 4043. [Abstract] [Full Text] [PDF] W.-K. You, H.-J. Seo, K.-H. Chung, and D.-S. Kim A Novel Metalloprotease from Gloydius halys Venom Induces Endothelial Cell Apoptosis through Its Protease and Disintegrin-Like Domains J. Biochem., November 1, 2003; 134(5): 739 - 749. [Abstract] [Full Text] [PDF] P. B. Armstrong and M. T. Armstrong The Decorated Clot: Binding of Agents of the Innate Immune System to the Fibrils of the Limulus Blood Clot Biol. Bull., October 1, 2003; 205(2): 201 - 203. [Full Text] [PDF] A. Luque, D. R. Carpizo, and M. L. Iruela-Arispe ADAMTS1/METH1 Inhibits Endothelial Cell Proliferation by Direct Binding and Sequestration of VEGF165 J. Biol. Chem., June 20, 2003; 278(26): 23656 - 23665. [Abstract] [Full Text] [PDF] E. S. Wijelath, J. Murray, S. Rahman, Y. Patel, A. Ishida, K. Strand, S. Aziz, C. Cardona, W. P. Hammond, G. F. Savidge, et al. Novel Vascular Endothelial Growth Factor Binding Domains of Fibronectin Enhance Vascular Endothelial Growth Factor Biological Activity Circ. Res., July 12, 2002; 91(1): 25 - 31. [Abstract] [Full Text] [PDF] A. F. Drew, H. Liu, J. M. Davidson, C. C. Daugherty, and J. L. Degen Wound-healing defects in mice lacking fibrinogen Blood, June 15, 2001; 97(12): 3691 - 3698. [Abstract] [Full Text] [PDF] A. Gorlach, I. Diebold, V. B. Schini-Kerth, U. Berchner-Pfannschmidt, U. Roth, R. P. Brandes, T. Kietzmann, and R. Busse Thrombin Activates the Hypoxia-Inducible Factor-1 Signaling Pathway in Vascular Smooth Muscle Cells : Role of the p22phox-Containing NADPH Oxidase Circ. Res., July 6, 2001; 89(1): 47 - 54. [Abstract] [Full Text] [PDF]

	Copyright © 2000 by American Society of Hematology         Online ISSN: 1528-0020