|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 December 2000, Vol. 96, No. 12, pp. 3857-3865
IMMUNOBIOLOGY
5-lipoxygenase expression in dendritic cells generated from
CD34+ hematopoietic progenitors and in lymphoid
organs
Rainer Spanbroek,
Markus Hildner,
Dieter Steinhilber,
Norbert Fusenig,
Kozo Yoneda,
Olof Rådmark, Bengt Samuelsson, and
Andreas J.R. Habenicht
From the Center for Vascular Medicine,
Friedrich Schiller University of Jena, Erfurt, Germany; the Department
of Pharmaceutical Chemistry, University of Frankfurt, Frankfurt,
Germany; the Division of Carcinogenesis and Differentiation, German
Cancer Research Center, Heidelberg, Germany; the Department of
Dermatology, Faculty of Medicine, Kyoto University, Kyoto, Japan; and
the Department of Medical Biochemistry and Biophysics, Division of
Chemistry II, Karolinska Institutet, Stockholm, Sweden.
The 5-lipoxygenase (5-LO) pathway in human
CD34+ hematopoietic progenitor cells, which were induced to
differentiate into dendritic cells (DCs) by cytokines in vitro and in
DCs of lymphoid tissues in situ, was examined. Extracts prepared from
HPCs contained low levels of 5-LO or 5-LO-activating protein.
Granulocyte-macrophage colony-stimulating factor (GM-CSF) plus tumor
necrosis factor- (TNF-) promoted DC differentiation and induced
a strong rise in 5-LO and FLAP expression. Fluorescence-activated cell
sorter (FACS) analyses identified a major DC population coexpressing human leukocyte antigen (HLA)-DR/CD80 and monocytic or Langerhans cell
markers. Transforming growth factor- 1 (TGF--1), added to support DC maturation, strongly promoted the appearance of
CD1a+/Lag+ Langerhans-type cells as well as
mature CD83+ DCs. TGF--1 further increased 5-LO and
FLAP expression, recruited additional cells into the 5-LO+
DC population, and promoted production of
5-hydroxyeicosatetraenoic acid and leukotriene B4 in
response to calcium (Ca++) ionophore A23187. These
in vitro findings were corroborated by 5-LO expression in distinct DC
phenotypes in vivo. Scattered 5-LO and FLAP in situ hybridization
signals were recorded in cells of paracortical T-lymphocyte-rich areas
and germinal centers (GCs) of lymph nodes (LNs) and tonsil and in cells
of mucosae overlying the Waldeyer tonsillar ring. 5-LO protein
localized to both CD1a+ immature DCs and to
CD83+ mature interdigitating DCs of T-lymphocyte-rich
areas of LNs and tonsil. As DCs have the unique ability to initiate
naive lymphocyte activation, our data support the hypothesis that
leukotrienes act at proximal steps of adaptive immune responses.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Liu, H.-E. Claesson, Y. Mahshid, G. Klein, and E. Klein
Leukotriene B4 activates T cells that inhibit B-cell proliferation in EBV-infected cord blood-derived mononuclear cell cultures
Blood,
March 1, 2008;
111(5):
2693 - 2703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. van de Ven, M. C. de Jong, A. W. Reurs, A. J. N. Schoonderwoerd, G. Jansen, J. H. Hooijberg, G. L. Scheffer, T. D. de Gruijl, and R. J. Scheper
Dendritic Cells Require Multidrug Resistance Protein 1 (ABCC1) Transporter Activity for Differentiation
J. Immunol.,
May 1, 2006;
176(9):
5191 - 5198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Christmas, K. Tolentino, V. Primo, K. Z. Berry, R. C. Murphy, M. Chen, D. M. Lee, and R. J. Soberman
Cytochrome P-450 4F18 Is the Leukotriene B4 {omega}-1/{omega}-2 Hydroxylase in Mouse Polymorphonuclear Leukocytes: IDENTIFICATION AS THE FUNCTIONAL ORTHOLOGUE OF HUMAN POLYMORPHONUCLEAR LEUKOCYTE CYP4F3A IN THE DOWN-REGULATION OF RESPONSES TO LTB4
J. Biol. Chem.,
March 17, 2006;
281(11):
7189 - 7196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Islam, S. Y. Thomas, C. Hess, B. D. Medoff, T. K. Means, C. Brander, C. M. Lilly, A. M. Tager, and A. D. Luster
The leukotriene B4 lipid chemoattractant receptor BLT1 defines antigen-primed T cells in humans
Blood,
January 15, 2006;
107(2):
444 - 453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. R. Schmelzer, L. Kubala, J. W. Newman, I.-H. Kim, J. P. Eiserich, and B. D. Hammock
Soluble epoxide hydrolase is a therapeutic target for acute inflammation
PNAS,
July 12, 2005;
102(28):
9772 - 9777.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Okunishi, M. Dohi, K. Nakagome, R. Tanaka, and K. Yamamoto
A Novel Role of Cysteinyl Leukotrienes to Promote Dendritic Cell Activation in the Antigen-Induced Immune Responses in the Lung
J. Immunol.,
November 15, 2004;
173(10):
6393 - 6402.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Machida, H. Matsuse, Y. Kondo, T. Kawano, S. Saeki, S. Tomari, Y. Obase, C. Fukushima, and S. Kohno
Cysteinyl Leukotrienes Regulate Dendritic Cell Functions in a Murine Model of Asthma
J. Immunol.,
February 1, 2004;
172(3):
1833 - 1838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Spanbroek, R. Grabner, K. Lotzer, M. Hildner, A. Urbach, K. Ruhling, M. P. W. Moos, B. Kaiser, T. U. Cohnert, T. Wahlers, et al.
Expanding expression of the 5-lipoxygenase pathway within the arterial wall during human atherogenesis
PNAS,
February 4, 2003;
100(3):
1238 - 1243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Harizi, M. Juzan, J.-F. Moreau, and N. Gualde
Prostaglandins Inhibit 5-Lipoxygenase-Activating Protein Expression and Leukotriene B4 Production from Dendritic Cells Via an IL-10-Dependent Mechanism
J. Immunol.,
January 1, 2003;
170(1):
139 - 146.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Werz, D. Szellas, D. Steinhilber, and O. Radmark
Arachidonic Acid Promotes Phosphorylation of 5-Lipoxygenase at Ser-271 by MAPK-activated Protein Kinase 2 (MK2)
J. Biol. Chem.,
April 19, 2002;
277(17):
14793 - 14800.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Werz, J. Klemm, O. Radmark, and B. Samuelsson
p38 MAP kinase mediates stress-induced leukotriene synthesis in a human B-lymphocyte cell line
J. Leukoc. Biol.,
November 1, 2001;
70(5):
830 - 838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Dubois, C. Massacrier, and C. Caux
Selective attraction of naive and memory B cells by dendritic cells
J. Leukoc. Biol.,
October 1, 2001;
70(4):
633 - 641.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Spanbroek, M. Hildner, A. Kohler, A. Muller, F. Zintl, H. Kuhn, O. Radmark, B. Samuelsson, and A. J. R. Habenicht
IL-4 determines eicosanoid formation in dendritic cells by down-regulation of 5-lipoxygenase and up-regulation of 15-lipoxygenase 1 expression
PNAS,
April 24, 2001;
98(9):
5152 - 5157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
