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Blood, 1 December 2000, Vol. 96, No. 12, pp. 3953-3957
PHAGOCYTES
Representational difference analysis using myeloid cells from
C/EBP deletional mice
Tetsuya Kubota,
Seiji Kawano,
Doris Y. Chih,
Yasuko Hisatake,
Alexey M. Chumakov,
Hirokuni Taguchi, and
H. Phillip Koeffler
From the Division of Hematology/Oncology, Cedars-Sinai
Research Institute, UCLA School of Medicine, Los Angeles, CA; and
Department of Medicine, Kochi Medical School, Kochi,
Japan.
C/EBP is a recently cloned member of the C/EBP family of
transcriptional factors. Previous studies demonstrated that the expression of this gene is tightly regulated in a tissue specific manner; it is expressed exclusively in myeloid cells.
C/EBP-deficient mice developed normally but failed to generate
functional neutrophils and eosinophils, and these mice died of
opportunistic infections suggesting that C/EBP may play a central
role in myeloid differentiation. To identify myelomonocytic genes
regulated by the C/EBP gene, we performed representational
difference analysis (RDA), a polymerase chain reaction (PCR)-based
subtractive hybridization using neutrophils and macrophages from
wild-type and C/EBP knockout mice. We identified a set of
differentially expressed genes, including chemokines specific to
myelomonocytic cells. Several novel genes were identified that were
differentially expressed in normal myelomonocytic cells. Taken
together, we have found several genes whose expression might be
enhanced by C/EBP.
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